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Gill Na+-K+-ATPase activity correlates with basolateral membrane lipid composition in seawater- but not freshwater-acclimated Arctic char (Salvelinus alpinus).
Am J Physiol Regul Integr Comp Physiol. 2007 Feb; 292(2):R1043-51.AJ

Abstract

The successful migration of euryhaline teleost fish from freshwater to seawater requires the upregulation of gill Na+-K+-ATPase, an ion transport enzyme located in the basolateral membrane (BLM) of gill chloride cells. Following 39 days of seawater exposure, Arctic char had similar plasma sodium and chloride levels as individuals maintained in freshwater, indicating they had successfully acclimated to seawater. This acclimation was associated with an eightfold increase in gill Na+-K+-ATPase activity but only a threefold increase in gill Na+-K+-ATPase protein number, suggesting that other mechanisms may also modulate gill Na+-K+-ATPase activity. We therefore investigated the influence of membrane composition on Na+-K+-ATPase activity by examining the phospholipid, fatty acid, and cholesterol composition of the gill BLM from freshwater- and seawater-acclimated Arctic char. Mean gill BLM cholesterol content was significantly lower (approximately 22%) in seawater-acclimated char. Gill Na+-K+-ATPase activity in individual seawater Arctic char was negatively correlated with BLM cholesterol content and positively correlated with %phosphatidylethanolamine and overall %18:2n6 (linoleic acid) content of the BLM, suggesting gill Na+-K+-ATPase activity of seawater-acclimated char may be modulated by the lipid composition of the BLM and may be especially sensitive to those parameters known to influence membrane fluidity. Na+-K+-ATPase activity of individual freshwater Arctic char was not correlated to any membrane lipid parameter measured, suggesting that different lipid-protein interactions may exist for char living in each environment.

Authors+Show Affiliations

Department Integrative Biology, University of Guelph, Guelph, Ontario, Canada, N1G 2W1.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17038447

Citation

Bystriansky, J S., and J S. Ballantyne. "Gill Na+-K+-ATPase Activity Correlates With Basolateral Membrane Lipid Composition in Seawater- but Not Freshwater-acclimated Arctic Char (Salvelinus Alpinus)." American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, vol. 292, no. 2, 2007, pp. R1043-51.
Bystriansky JS, Ballantyne JS. Gill Na+-K+-ATPase activity correlates with basolateral membrane lipid composition in seawater- but not freshwater-acclimated Arctic char (Salvelinus alpinus). Am J Physiol Regul Integr Comp Physiol. 2007;292(2):R1043-51.
Bystriansky, J. S., & Ballantyne, J. S. (2007). Gill Na+-K+-ATPase activity correlates with basolateral membrane lipid composition in seawater- but not freshwater-acclimated Arctic char (Salvelinus alpinus). American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 292(2), R1043-51.
Bystriansky JS, Ballantyne JS. Gill Na+-K+-ATPase Activity Correlates With Basolateral Membrane Lipid Composition in Seawater- but Not Freshwater-acclimated Arctic Char (Salvelinus Alpinus). Am J Physiol Regul Integr Comp Physiol. 2007;292(2):R1043-51. PubMed PMID: 17038447.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gill Na+-K+-ATPase activity correlates with basolateral membrane lipid composition in seawater- but not freshwater-acclimated Arctic char (Salvelinus alpinus). AU - Bystriansky,J S, AU - Ballantyne,J S, Y1 - 2006/10/12/ PY - 2006/10/14/pubmed PY - 2007/3/9/medline PY - 2006/10/14/entrez SP - R1043 EP - 51 JF - American journal of physiology. Regulatory, integrative and comparative physiology JO - Am. J. Physiol. Regul. Integr. Comp. Physiol. VL - 292 IS - 2 N2 - The successful migration of euryhaline teleost fish from freshwater to seawater requires the upregulation of gill Na+-K+-ATPase, an ion transport enzyme located in the basolateral membrane (BLM) of gill chloride cells. Following 39 days of seawater exposure, Arctic char had similar plasma sodium and chloride levels as individuals maintained in freshwater, indicating they had successfully acclimated to seawater. This acclimation was associated with an eightfold increase in gill Na+-K+-ATPase activity but only a threefold increase in gill Na+-K+-ATPase protein number, suggesting that other mechanisms may also modulate gill Na+-K+-ATPase activity. We therefore investigated the influence of membrane composition on Na+-K+-ATPase activity by examining the phospholipid, fatty acid, and cholesterol composition of the gill BLM from freshwater- and seawater-acclimated Arctic char. Mean gill BLM cholesterol content was significantly lower (approximately 22%) in seawater-acclimated char. Gill Na+-K+-ATPase activity in individual seawater Arctic char was negatively correlated with BLM cholesterol content and positively correlated with %phosphatidylethanolamine and overall %18:2n6 (linoleic acid) content of the BLM, suggesting gill Na+-K+-ATPase activity of seawater-acclimated char may be modulated by the lipid composition of the BLM and may be especially sensitive to those parameters known to influence membrane fluidity. Na+-K+-ATPase activity of individual freshwater Arctic char was not correlated to any membrane lipid parameter measured, suggesting that different lipid-protein interactions may exist for char living in each environment. SN - 0363-6119 UR - https://www.unboundmedicine.com/medline/citation/17038447/Gill_Na+_K+_ATPase_activity_correlates_with_basolateral_membrane_lipid_composition_in_seawater__but_not_freshwater_acclimated_Arctic_char__Salvelinus_alpinus__ L2 - http://www.physiology.org/doi/full/10.1152/ajpregu.00189.2005?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -