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Quantification of Delta9-tetrahydrocannabinol and its major metabolites in meconium by gas chromatographic-mass spectrometric assay: assay validation and preliminary results of the "meconium project".
Ther Drug Monit. 2006 Oct; 28(5):700-6.TD

Abstract

A rapid and simple procedure based on gas chromatography-mass spectrometry (GC-MS) is described for determination of Delta-tetrahydrocannabinol (THC), 11-hydroxy-Delta-tetrahydrocannabinol (THC-OH) and 11-nor-Delta-tetrahydrocannabinol-9-carboxylic acid (THC-COOH) in meconium using Delta-tetrahydrocannabinol (Delta-THC) and deuterated THC-COOH as internal standards. The biological matrix was subjected to liquid-liquid extraction after enzyme hydrolysis for conjugated analytes.Chromatography was performed on a fused silica capillary column and analytes were determined in the selected-ion-monitoring (SIM) mode. The method was validated in the range 20 to 500 microg/g using 1g of meconium per assay. The method was applied to the analysis of meconium in a cohort of newborns to assess eventual fetal exposure to cannabis. Within positive samples, THC-COOH and THC-OH (range: 33.7 to 182.1 and 20.7 to 493.3 microg/g, respectively) were both present in the majority of cases with only 1 specimen with THC-OH as the most abundant metabolite and 2 with THC only.

Authors+Show Affiliations

Drug Research and Evaluation Department, Istituto Superiore di Sanità, V.le Regina Elena 299, 00161 Rome, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17038890

Citation

Marchei, Emilia, et al. "Quantification of Delta9-tetrahydrocannabinol and Its Major Metabolites in Meconium By Gas Chromatographic-mass Spectrometric Assay: Assay Validation and Preliminary Results of the "meconium Project"." Therapeutic Drug Monitoring, vol. 28, no. 5, 2006, pp. 700-6.
Marchei E, Pellegrini M, Pacifici R, et al. Quantification of Delta9-tetrahydrocannabinol and its major metabolites in meconium by gas chromatographic-mass spectrometric assay: assay validation and preliminary results of the "meconium project". Ther Drug Monit. 2006;28(5):700-6.
Marchei, E., Pellegrini, M., Pacifici, R., Palmi, I., Lozano, J., García-Algar, O., & Pichini, S. (2006). Quantification of Delta9-tetrahydrocannabinol and its major metabolites in meconium by gas chromatographic-mass spectrometric assay: assay validation and preliminary results of the "meconium project". Therapeutic Drug Monitoring, 28(5), 700-6.
Marchei E, et al. Quantification of Delta9-tetrahydrocannabinol and Its Major Metabolites in Meconium By Gas Chromatographic-mass Spectrometric Assay: Assay Validation and Preliminary Results of the "meconium Project". Ther Drug Monit. 2006;28(5):700-6. PubMed PMID: 17038890.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantification of Delta9-tetrahydrocannabinol and its major metabolites in meconium by gas chromatographic-mass spectrometric assay: assay validation and preliminary results of the "meconium project". AU - Marchei,Emilia, AU - Pellegrini,Manuela, AU - Pacifici,Roberta, AU - Palmi,Ilaria, AU - Lozano,Jaime, AU - García-Algar,Oscar, AU - Pichini,Simona, PY - 2006/10/14/pubmed PY - 2007/1/11/medline PY - 2006/10/14/entrez SP - 700 EP - 6 JF - Therapeutic drug monitoring JO - Ther Drug Monit VL - 28 IS - 5 N2 - A rapid and simple procedure based on gas chromatography-mass spectrometry (GC-MS) is described for determination of Delta-tetrahydrocannabinol (THC), 11-hydroxy-Delta-tetrahydrocannabinol (THC-OH) and 11-nor-Delta-tetrahydrocannabinol-9-carboxylic acid (THC-COOH) in meconium using Delta-tetrahydrocannabinol (Delta-THC) and deuterated THC-COOH as internal standards. The biological matrix was subjected to liquid-liquid extraction after enzyme hydrolysis for conjugated analytes.Chromatography was performed on a fused silica capillary column and analytes were determined in the selected-ion-monitoring (SIM) mode. The method was validated in the range 20 to 500 microg/g using 1g of meconium per assay. The method was applied to the analysis of meconium in a cohort of newborns to assess eventual fetal exposure to cannabis. Within positive samples, THC-COOH and THC-OH (range: 33.7 to 182.1 and 20.7 to 493.3 microg/g, respectively) were both present in the majority of cases with only 1 specimen with THC-OH as the most abundant metabolite and 2 with THC only. SN - 0163-4356 UR - https://www.unboundmedicine.com/medline/citation/17038890/Quantification_of_Delta9_tetrahydrocannabinol_and_its_major_metabolites_in_meconium_by_gas_chromatographic_mass_spectrometric_assay:_assay_validation_and_preliminary_results_of_the_"meconium_project"_ L2 - https://doi.org/10.1097/01.ftd.0000245380.95186.13 DB - PRIME DP - Unbound Medicine ER -