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[Rapid and sensitive determination of the adenylate energy charge in stored erythrocytes using high pressure liquid chromatography].
Beitr Infusionsther. 1990; 26:86-8.BI

Abstract

Using reversed-phase high-performance liquid chromatography (HPLC), we developed a fast method for the determination of the adenine-nucleotides ATP, ADP, and AMP in human red blood cells (RBC). The method is sensitive (10(-9)-10(-10) M), specific (greater than 95% peak-homogeneity), and the results are well reproducible. The three substances are assayed in a single step, which is essential for the exact determination of the adenylate-energy-charge (AEC). We applied the technique to fresh and stored RBC's. After six weeks' storage in PAGGS-sorbitol we found only a slight decrease in ATP content, but marked increases in ADP and AMP contents. Accordingly, there was a decrease in the AEC, whereas the total nucleotide pool (TNP) did not change significantly. As AEC and TNP reflect the state of the cellular energy metabolism, they are related to the viability of stored RBC. Thus, we consider the described method to be helpful for the quality control of RBC stored in new or modified media.

Authors+Show Affiliations

Sibrowski W
Abt. f. Transfusionsmedizin u. Transplantationsimmunologie, Universitäts-Krankenhaus Eppendorf, Hamburg.
Lüdemann K
No affiliation info available
Dominka T
No affiliation info available
von Eisenhart-Rothe B
No affiliation info available
Kühnl P
No affiliation info available

MeSH

AdenineAdenosine DiphosphateAdenosine MonophosphateAdenosine TriphosphateBlood PreservationBlood TransfusionChromatography, High Pressure LiquidErythrocyte TransfusionErythrocytesGlucoseGuanosineHumansPhosphatesSodium ChlorideSorbitol

Pub Type(s)

English Abstract
Journal Article

Language

ger

PubMed ID

1703908

Citation

Sibrowski, W, et al. "[Rapid and Sensitive Determination of the Adenylate Energy Charge in Stored Erythrocytes Using High Pressure Liquid Chromatography]." Beitrage Zur Infusionstherapie = Contributions to Infusion Therapy, vol. 26, 1990, pp. 86-8.
Sibrowski W, Lüdemann K, Dominka T, et al. [Rapid and sensitive determination of the adenylate energy charge in stored erythrocytes using high pressure liquid chromatography]. Beitr Infusionsther. 1990;26:86-8.
Sibrowski, W., Lüdemann, K., Dominka, T., von Eisenhart-Rothe, B., & Kühnl, P. (1990). [Rapid and sensitive determination of the adenylate energy charge in stored erythrocytes using high pressure liquid chromatography]. Beitrage Zur Infusionstherapie = Contributions to Infusion Therapy, 26, 86-8.
Sibrowski W, et al. [Rapid and Sensitive Determination of the Adenylate Energy Charge in Stored Erythrocytes Using High Pressure Liquid Chromatography]. Beitr Infusionsther. 1990;26:86-8. PubMed PMID: 1703908.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Rapid and sensitive determination of the adenylate energy charge in stored erythrocytes using high pressure liquid chromatography]. AU - Sibrowski,W, AU - Lüdemann,K, AU - Dominka,T, AU - von Eisenhart-Rothe,B, AU - Kühnl,P, PY - 1990/1/1/pubmed PY - 1990/1/1/medline PY - 1990/1/1/entrez SP - 86 EP - 8 JF - Beitrage zur Infusionstherapie = Contributions to infusion therapy JO - Beitr Infusionsther VL - 26 N2 - Using reversed-phase high-performance liquid chromatography (HPLC), we developed a fast method for the determination of the adenine-nucleotides ATP, ADP, and AMP in human red blood cells (RBC). The method is sensitive (10(-9)-10(-10) M), specific (greater than 95% peak-homogeneity), and the results are well reproducible. The three substances are assayed in a single step, which is essential for the exact determination of the adenylate-energy-charge (AEC). We applied the technique to fresh and stored RBC's. After six weeks' storage in PAGGS-sorbitol we found only a slight decrease in ATP content, but marked increases in ADP and AMP contents. Accordingly, there was a decrease in the AEC, whereas the total nucleotide pool (TNP) did not change significantly. As AEC and TNP reflect the state of the cellular energy metabolism, they are related to the viability of stored RBC. Thus, we consider the described method to be helpful for the quality control of RBC stored in new or modified media. SN - 1011-6974 UR - https://www.unboundmedicine.com/medline/citation/1703908/[Rapid_and_sensitive_determination_of_the_adenylate_energy_charge_in_stored_erythrocytes_using_high_pressure_liquid_chromatography]_ DB - PRIME DP - Unbound Medicine ER -