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Genotyping by RAPD-PCR analyses of Malassezia furfur strains from pityriasis versicolor and seborrhoeic dermatitis patients.
Mycopathologia. 2006 Oct; 162(4):273-80.M

Abstract

Malassezia furfur is lypophilic yeast commonly associate with dermatological disorders. In the present work, we described the isolation of 47 M. furfur strains from three groups of patients: pityriasis versicolor (21 isolates), seborrhoeic dermatitis (15 isolates) and seborrhoeic dermatitis of the HIV positive patients (11 isolates). To investigate the identity of the strains at molecular level, DNA genomic of M. furfur strains were prepared and used to RAPD-PCR analyses. RAPD assay were carried out using two decamer primers and bands pattern generated were analyzed by an Unweighted Pair-Group Method (UPGMA). Dendrogram established a distinct differentiation between M. furfur isolates from pityriasis versicolor and seborrhoeic dermatitis patients with or without AIDS. We concluded that RAPD typing presented a high discriminatory power between strains studied in this work and can be applied in epidemiological investigation of skin disease causing by M. furfur.

Authors+Show Affiliations

Gandra RF
Centro de Ciências Médicas e Farmacêuticas, Universidade Estadual do Oeste do Paraná, 2069, Cascavel, 85814-110, PR, Brazil. rfgandra@unioeste.br
Simão RC
No affiliation info available
Matsumoto FE
No affiliation info available
da Silva BC
No affiliation info available
Ruiz LS
No affiliation info available
da Silva EG
No affiliation info available
Gambale W
No affiliation info available
Paula CR
No affiliation info available

MeSH

Acquired Immunodeficiency SyndromeDNA, FungalDermatitis, SeborrheicGenotypeHIVHumansMalasseziaRandom Amplified Polymorphic DNA TechniqueTinea Versicolor

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17039273

Citation

Gandra, Rinaldo F., et al. "Genotyping By RAPD-PCR Analyses of Malassezia Furfur Strains From Pityriasis Versicolor and Seborrhoeic Dermatitis Patients." Mycopathologia, vol. 162, no. 4, 2006, pp. 273-80.
Gandra RF, Simão RC, Matsumoto FE, et al. Genotyping by RAPD-PCR analyses of Malassezia furfur strains from pityriasis versicolor and seborrhoeic dermatitis patients. Mycopathologia. 2006;162(4):273-80.
Gandra, R. F., Simão, R. C., Matsumoto, F. E., da Silva, B. C., Ruiz, L. S., da Silva, E. G., Gambale, W., & Paula, C. R. (2006). Genotyping by RAPD-PCR analyses of Malassezia furfur strains from pityriasis versicolor and seborrhoeic dermatitis patients. Mycopathologia, 162(4), 273-80.
Gandra RF, et al. Genotyping By RAPD-PCR Analyses of Malassezia Furfur Strains From Pityriasis Versicolor and Seborrhoeic Dermatitis Patients. Mycopathologia. 2006;162(4):273-80. PubMed PMID: 17039273.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotyping by RAPD-PCR analyses of Malassezia furfur strains from pityriasis versicolor and seborrhoeic dermatitis patients. AU - Gandra,Rinaldo F, AU - Simão,Rita C G, AU - Matsumoto,Flávia E, AU - da Silva,Bosco C M, AU - Ruiz,Luciana S, AU - da Silva,Eriques G, AU - Gambale,Walderez, AU - Paula,Claudete R, PY - 2006/03/28/received PY - 2006/08/17/accepted PY - 2006/10/14/pubmed PY - 2007/1/12/medline PY - 2006/10/14/entrez SP - 273 EP - 80 JF - Mycopathologia JO - Mycopathologia VL - 162 IS - 4 N2 - Malassezia furfur is lypophilic yeast commonly associate with dermatological disorders. In the present work, we described the isolation of 47 M. furfur strains from three groups of patients: pityriasis versicolor (21 isolates), seborrhoeic dermatitis (15 isolates) and seborrhoeic dermatitis of the HIV positive patients (11 isolates). To investigate the identity of the strains at molecular level, DNA genomic of M. furfur strains were prepared and used to RAPD-PCR analyses. RAPD assay were carried out using two decamer primers and bands pattern generated were analyzed by an Unweighted Pair-Group Method (UPGMA). Dendrogram established a distinct differentiation between M. furfur isolates from pityriasis versicolor and seborrhoeic dermatitis patients with or without AIDS. We concluded that RAPD typing presented a high discriminatory power between strains studied in this work and can be applied in epidemiological investigation of skin disease causing by M. furfur. SN - 0301-486X UR - https://www.unboundmedicine.com/medline/citation/17039273/Genotyping_by_RAPD_PCR_analyses_of_Malassezia_furfur_strains_from_pityriasis_versicolor_and_seborrhoeic_dermatitis_patients_ DB - PRIME DP - Unbound Medicine ER -