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Effects of trolox on nerve dysfunction, thermal hyperalgesia and oxidative stress in experimental diabetic neuropathy.

Abstract

1. Diabetic neuropathy is one of the most common complications of diabetes and oxidative stress has been implicated to play a major role in its pathophysiology. 2. In the present study, we targeted oxidative stress using trolox, an anti-oxidant, in streptozotocin-induced diabetic neuropathy in rats. 3. Compared with control rats, diabetic rats showed significant deficits in motor nerve conduction velocity (MNCV; 49.91 +/- 1.94 vs 42.77 +/- 1.39 m/s, respectively) and nerve blood flow (NBF; 107.98 +/- 8.22 vs 38.9 +/- 2.7 arbitarary perfusion units, respectively) after 8 weeks of diabetes. Tail flick latencies for cold and hot immersion tests were also significantly reduced in diabetic rats, indicating thermal hyperalgesia. These observations indicate development of diabetic neuropathy. 4. A significant decrease in the activity of anti-oxidant enzymes (superoxide dismutase and catalase) and an increase in lipid peroxidation were observed in sciatic nerves from diabetic rats compared with age-matched control rats. Alterations in the activity of anti-oxidant enzymes and lipid peroxidation in diabetic rats indicate oxidative stress in diabetic neuropathy. 5. Two weeks treatment with trolox (10 and 30 mg/kg, i.p.) started on completion of the 6th week of diabetes significantly improved MNCV, NBF and inhibited thermal hyperalgesia. Trolox treatment also improved the activity of anti-oxidant enzymes and inhibited lipid peroxidation in sciatic nerves of diabetic rats. 6. The results of the present study suggest the beneficial effects of trolox in experimental diabetic neuropathy.

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  • Authors+Show Affiliations

    ,

    Molecular Neuropharmacology Laboratory, Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), Punjab, India. ssharma@niper.ac.in

    Source

    MeSH

    Animals
    Blood Glucose
    Catalase
    Chromans
    Diabetes Mellitus, Experimental
    Diabetic Neuropathies
    Dose-Response Relationship, Drug
    Hyperalgesia
    Lipid Peroxidation
    Male
    Neural Conduction
    Oxidative Stress
    Rats
    Rats, Sprague-Dawley
    Sciatic Nerve
    Superoxide Dismutase

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    17042909

    Citation

    Sharma, Shyam S., and Sufyan G. Sayyed. "Effects of Trolox On Nerve Dysfunction, Thermal Hyperalgesia and Oxidative Stress in Experimental Diabetic Neuropathy." Clinical and Experimental Pharmacology & Physiology, vol. 33, no. 11, 2006, pp. 1022-8.
    Sharma SS, Sayyed SG. Effects of trolox on nerve dysfunction, thermal hyperalgesia and oxidative stress in experimental diabetic neuropathy. Clin Exp Pharmacol Physiol. 2006;33(11):1022-8.
    Sharma, S. S., & Sayyed, S. G. (2006). Effects of trolox on nerve dysfunction, thermal hyperalgesia and oxidative stress in experimental diabetic neuropathy. Clinical and Experimental Pharmacology & Physiology, 33(11), pp. 1022-8.
    Sharma SS, Sayyed SG. Effects of Trolox On Nerve Dysfunction, Thermal Hyperalgesia and Oxidative Stress in Experimental Diabetic Neuropathy. Clin Exp Pharmacol Physiol. 2006;33(11):1022-8. PubMed PMID: 17042909.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Effects of trolox on nerve dysfunction, thermal hyperalgesia and oxidative stress in experimental diabetic neuropathy. AU - Sharma,Shyam S, AU - Sayyed,Sufyan G, PY - 2006/10/18/pubmed PY - 2007/2/3/medline PY - 2006/10/18/entrez SP - 1022 EP - 8 JF - Clinical and experimental pharmacology & physiology JO - Clin. Exp. Pharmacol. Physiol. VL - 33 IS - 11 N2 - 1. Diabetic neuropathy is one of the most common complications of diabetes and oxidative stress has been implicated to play a major role in its pathophysiology. 2. In the present study, we targeted oxidative stress using trolox, an anti-oxidant, in streptozotocin-induced diabetic neuropathy in rats. 3. Compared with control rats, diabetic rats showed significant deficits in motor nerve conduction velocity (MNCV; 49.91 +/- 1.94 vs 42.77 +/- 1.39 m/s, respectively) and nerve blood flow (NBF; 107.98 +/- 8.22 vs 38.9 +/- 2.7 arbitarary perfusion units, respectively) after 8 weeks of diabetes. Tail flick latencies for cold and hot immersion tests were also significantly reduced in diabetic rats, indicating thermal hyperalgesia. These observations indicate development of diabetic neuropathy. 4. A significant decrease in the activity of anti-oxidant enzymes (superoxide dismutase and catalase) and an increase in lipid peroxidation were observed in sciatic nerves from diabetic rats compared with age-matched control rats. Alterations in the activity of anti-oxidant enzymes and lipid peroxidation in diabetic rats indicate oxidative stress in diabetic neuropathy. 5. Two weeks treatment with trolox (10 and 30 mg/kg, i.p.) started on completion of the 6th week of diabetes significantly improved MNCV, NBF and inhibited thermal hyperalgesia. Trolox treatment also improved the activity of anti-oxidant enzymes and inhibited lipid peroxidation in sciatic nerves of diabetic rats. 6. The results of the present study suggest the beneficial effects of trolox in experimental diabetic neuropathy. SN - 0305-1870 UR - https://www.unboundmedicine.com/medline/citation/17042909/Effects_of_trolox_on_nerve_dysfunction_thermal_hyperalgesia_and_oxidative_stress_in_experimental_diabetic_neuropathy_ L2 - https://doi.org/10.1111/j.1440-1681.2006.04481.x DB - PRIME DP - Unbound Medicine ER -