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Effect of all-trans retinoic acid on sodium/iodide symporter expression, radioiodine uptake and gene expression profiles in a human anaplastic thyroid carcinoma cell line.

Abstract

The plasma membrane glycoprotein sodium/iodide symporter (NIS) is crucial for thyroid hormone biosynthesis and mediates the iodide uptake of thyrocytes. It has been shown that retinoic acid (RA) alters NIS gene expression in thyroid carcinoma lines and stimulates their iodide uptake. Here, we generated an ARO human thyroidal cancer cell line that expresses the NIS gene (ARO-NIS) and found that its baseline 125I uptake was threefold higher than that of its parental ARO cells. However, a 1-microM all-trans retinoic acid (tRA) treatment significantly increased this 125I uptake up to approximately approximately 6.5-fold on Day 3. tRA also elevated NIS mRNA expression in ARO-NIS cells, with peaks of expression being observed on Day 3. To investigate the underlying genomic mechanisms involved in these tRA-induced phenotypic changes, we subjected tRA-treated and untreated ARO-NIS cells to cDNA microarray analysis. Of 1152, genes spotted onto the microarray membrane, 18 were up-regulated (z ratio>2.0) and 33 were down-regulated (z ratio<-2.0) in ARO-NIS cells after 3 days of tRA treatment. More specifically, tRA increased the expression of BCL3, CSRP3, v-fos, and CDK5 genes and decreased the expression of the FGF12 and IGFBP6 genes. Thus, tRA treatment of human anaplastic thyroid carcinoma cells stably expressing the NIS gene significantly elevates their NIS-mediated radioiodine uptake and alters the expression of many genes involved in cell growth and cellular differentiation. Therefore, tRA treatment and NIS gene transfection are potential tools for the diagnosis and treatment of thyroid cancer.

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  • Authors+Show Affiliations

    ,

    Department of Nuclear Medicine, College of Medicine, Wonkwang University, Iksan, Jellabuk-do 570-711, South Korea.

    , , , ,

    Source

    Nuclear medicine and biology 33:7 2006 Oct pg 875-82

    MeSH

    Carcinoma
    Cell Line, Tumor
    Dose-Response Relationship, Drug
    Gene Expression
    Gene Expression Profiling
    Gene Expression Regulation, Neoplastic
    Humans
    Iodine Radioisotopes
    Metabolic Clearance Rate
    Neoplasm Proteins
    Radiopharmaceuticals
    Symporters
    Thyroid Neoplasms
    Tretinoin

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    17045167

    Citation

    Jeong, Hwanjeong, et al. "Effect of All-trans Retinoic Acid On Sodium/iodide Symporter Expression, Radioiodine Uptake and Gene Expression Profiles in a Human Anaplastic Thyroid Carcinoma Cell Line." Nuclear Medicine and Biology, vol. 33, no. 7, 2006, pp. 875-82.
    Jeong H, Kim YR, Kim KN, et al. Effect of all-trans retinoic acid on sodium/iodide symporter expression, radioiodine uptake and gene expression profiles in a human anaplastic thyroid carcinoma cell line. Nucl Med Biol. 2006;33(7):875-82.
    Jeong, H., Kim, Y. R., Kim, K. N., Choe, J. G., Chung, J. K., & Kim, M. K. (2006). Effect of all-trans retinoic acid on sodium/iodide symporter expression, radioiodine uptake and gene expression profiles in a human anaplastic thyroid carcinoma cell line. Nuclear Medicine and Biology, 33(7), pp. 875-82.
    Jeong H, et al. Effect of All-trans Retinoic Acid On Sodium/iodide Symporter Expression, Radioiodine Uptake and Gene Expression Profiles in a Human Anaplastic Thyroid Carcinoma Cell Line. Nucl Med Biol. 2006;33(7):875-82. PubMed PMID: 17045167.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Effect of all-trans retinoic acid on sodium/iodide symporter expression, radioiodine uptake and gene expression profiles in a human anaplastic thyroid carcinoma cell line. AU - Jeong,Hwanjeong, AU - Kim,Yu-Ri, AU - Kim,Ki-Nam, AU - Choe,Jae-Gol, AU - Chung,June-Key, AU - Kim,Meyoung-Kon, PY - 2006/06/07/received PY - 2006/07/06/accepted PY - 2006/10/19/pubmed PY - 2007/1/11/medline PY - 2006/10/19/entrez SP - 875 EP - 82 JF - Nuclear medicine and biology JO - Nucl. Med. Biol. VL - 33 IS - 7 N2 - The plasma membrane glycoprotein sodium/iodide symporter (NIS) is crucial for thyroid hormone biosynthesis and mediates the iodide uptake of thyrocytes. It has been shown that retinoic acid (RA) alters NIS gene expression in thyroid carcinoma lines and stimulates their iodide uptake. Here, we generated an ARO human thyroidal cancer cell line that expresses the NIS gene (ARO-NIS) and found that its baseline 125I uptake was threefold higher than that of its parental ARO cells. However, a 1-microM all-trans retinoic acid (tRA) treatment significantly increased this 125I uptake up to approximately approximately 6.5-fold on Day 3. tRA also elevated NIS mRNA expression in ARO-NIS cells, with peaks of expression being observed on Day 3. To investigate the underlying genomic mechanisms involved in these tRA-induced phenotypic changes, we subjected tRA-treated and untreated ARO-NIS cells to cDNA microarray analysis. Of 1152, genes spotted onto the microarray membrane, 18 were up-regulated (z ratio>2.0) and 33 were down-regulated (z ratio<-2.0) in ARO-NIS cells after 3 days of tRA treatment. More specifically, tRA increased the expression of BCL3, CSRP3, v-fos, and CDK5 genes and decreased the expression of the FGF12 and IGFBP6 genes. Thus, tRA treatment of human anaplastic thyroid carcinoma cells stably expressing the NIS gene significantly elevates their NIS-mediated radioiodine uptake and alters the expression of many genes involved in cell growth and cellular differentiation. Therefore, tRA treatment and NIS gene transfection are potential tools for the diagnosis and treatment of thyroid cancer. SN - 0969-8051 UR - https://www.unboundmedicine.com/medline/citation/17045167/Effect_of_all_trans_retinoic_acid_on_sodium/iodide_symporter_expression_radioiodine_uptake_and_gene_expression_profiles_in_a_human_anaplastic_thyroid_carcinoma_cell_line_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0969-8051(06)00131-4 DB - PRIME DP - Unbound Medicine ER -