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An impedimetric immunosensor for the label-free detection of bisphenol A.
Biosens Bioelectron. 2007 May 15; 22(11):2464-70.BB

Abstract

Label-free detection of bisphenol A based on the impedance measurement was achieved with an impedimetric immunosensor. The immunosensor was fabricated by the covalent bond formation between a polyclonal antibody and a carboxylic acid group functionalized onto a nano-particle comprised conducting polymer. By using a commercial reagent 4,4-bis(4-hydroxyphenyl) valeric acid (BHPVA), which has an analogous structure of BPA, we have prepared the antigen through the conjugation of BHPVA with bovine serum albumin (BSA) and then produced a specific polyclonal antibody. The immobilization of antibody and the interaction between antibody and antigen were studied using quartz crystal microbalance (QCM) and electrochemical impedance spectroscopic (EIS) techniques. The impedance and mass changes due to the specific immuno-interaction at the sensor surface were utilized to detect antigen and bisphenol A (BPA). The immunosensor showed specific recognition of BPA with less interference than 4.5% from other common phenolic compounds. Under an optimized condition, the linear dynamic range of BPA detection was between 1 and 100 ng/ml. The detection limit of bisphenol A was determined to be 0.3+/-0.07 ng/ml. The proposed immunosensor was applied to a human serum sample and the BPA concentration was determined by the standard addition method.

Authors+Show Affiliations

Rahman MA
Department of Chemistry and Center for Innovative BioPhysio Sensor Technology, Pusan National University, Keumjeong-ku, Busan 609-735, South Korea.
Shiddiky MJ
No affiliation info available
Park JS
No affiliation info available
Shim YB
No affiliation info available

MeSH

Antibodies, MonoclonalBenzhydryl CompoundsBiosensing TechniquesElectric ImpedanceEquipment DesignEquipment Failure AnalysisImmunoassayPhenolsReproducibility of ResultsSensitivity and SpecificityStaining and Labeling

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17045793

Citation

Rahman, Md Aminur, et al. "An Impedimetric Immunosensor for the Label-free Detection of Bisphenol A." Biosensors & Bioelectronics, vol. 22, no. 11, 2007, pp. 2464-70.
Rahman MA, Shiddiky MJ, Park JS, et al. An impedimetric immunosensor for the label-free detection of bisphenol A. Biosens Bioelectron. 2007;22(11):2464-70.
Rahman, M. A., Shiddiky, M. J., Park, J. S., & Shim, Y. B. (2007). An impedimetric immunosensor for the label-free detection of bisphenol A. Biosensors & Bioelectronics, 22(11), 2464-70.
Rahman MA, et al. An Impedimetric Immunosensor for the Label-free Detection of Bisphenol A. Biosens Bioelectron. 2007 May 15;22(11):2464-70. PubMed PMID: 17045793.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An impedimetric immunosensor for the label-free detection of bisphenol A. AU - Rahman,Md Aminur, AU - Shiddiky,Muhammad J A, AU - Park,Jang-Su, AU - Shim,Yoon-Bo, Y1 - 2006/10/12/ PY - 2006/06/09/received PY - 2006/09/04/revised PY - 2006/09/07/accepted PY - 2006/10/19/pubmed PY - 2007/6/28/medline PY - 2006/10/19/entrez SP - 2464 EP - 70 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 22 IS - 11 N2 - Label-free detection of bisphenol A based on the impedance measurement was achieved with an impedimetric immunosensor. The immunosensor was fabricated by the covalent bond formation between a polyclonal antibody and a carboxylic acid group functionalized onto a nano-particle comprised conducting polymer. By using a commercial reagent 4,4-bis(4-hydroxyphenyl) valeric acid (BHPVA), which has an analogous structure of BPA, we have prepared the antigen through the conjugation of BHPVA with bovine serum albumin (BSA) and then produced a specific polyclonal antibody. The immobilization of antibody and the interaction between antibody and antigen were studied using quartz crystal microbalance (QCM) and electrochemical impedance spectroscopic (EIS) techniques. The impedance and mass changes due to the specific immuno-interaction at the sensor surface were utilized to detect antigen and bisphenol A (BPA). The immunosensor showed specific recognition of BPA with less interference than 4.5% from other common phenolic compounds. Under an optimized condition, the linear dynamic range of BPA detection was between 1 and 100 ng/ml. The detection limit of bisphenol A was determined to be 0.3+/-0.07 ng/ml. The proposed immunosensor was applied to a human serum sample and the BPA concentration was determined by the standard addition method. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/17045793/An_impedimetric_immunosensor_for_the_label_free_detection_of_bisphenol_A_ DB - PRIME DP - Unbound Medicine ER -