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Rapid method for accurate analysis of melatonin, serotonin and auxin in plant samples using liquid chromatography-tandem mass spectrometry.
J Chromatogr A. 2006 Nov 17; 1134(1-2):333-7.JC

Abstract

Currently, the information available on the physiological functions of melatonin in higher plants is rather limited and the role of plant melatonin in human health remains undetermined. Research in this area has been slow due to lack of efficient analytical methods for rapid identification and quantification of the melatonin and related compounds in complex plant matrices. In this communication, we report the development of a rapid, accurate method for extraction, detection and quantification of plant melatonin, serotonin and indole-3-acetic acid (IAA) by Liquid chromatography-tandem mass spectrometry (LC-MS/MS) with electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI), and atmospheric pressure photoionization (APPI), respectively. The limit of detection (LOD) of melatonin in the plant extraction was 5 pg/ml and the limit of quantification (LOQ) was 0.02 ng/ml, as well as LOD for serotonin was 100 pg/ml and the LOQ was 5 ng/ml, LOD for IAA was 50 pg/ml and the LOQ was 0.7 ng/ml. There was a linear relationship between melatonin, serotonin, and IAA concentration and peak area over a quantifiable range of 0.02 ng/ml to 0.1 mg/ml, 5 ng/ml to 0.1 mg/ml, and 0.7 ng/ml to 0.1 mg/ml, respectively, in the plant extract.

Authors+Show Affiliations

Chemistry, I.K. Barber School of Arts and Sciences, University of British Columbia Okanagan, 3333 University Way, Kelowna V1V 1V7, Canada.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17046004

Citation

Cao, Jin, et al. "Rapid Method for Accurate Analysis of Melatonin, Serotonin and Auxin in Plant Samples Using Liquid Chromatography-tandem Mass Spectrometry." Journal of Chromatography. A, vol. 1134, no. 1-2, 2006, pp. 333-7.
Cao J, Murch SJ, O'Brien R, et al. Rapid method for accurate analysis of melatonin, serotonin and auxin in plant samples using liquid chromatography-tandem mass spectrometry. J Chromatogr A. 2006;1134(1-2):333-7.
Cao, J., Murch, S. J., O'Brien, R., & Saxena, P. K. (2006). Rapid method for accurate analysis of melatonin, serotonin and auxin in plant samples using liquid chromatography-tandem mass spectrometry. Journal of Chromatography. A, 1134(1-2), 333-7.
Cao J, et al. Rapid Method for Accurate Analysis of Melatonin, Serotonin and Auxin in Plant Samples Using Liquid Chromatography-tandem Mass Spectrometry. J Chromatogr A. 2006 Nov 17;1134(1-2):333-7. PubMed PMID: 17046004.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid method for accurate analysis of melatonin, serotonin and auxin in plant samples using liquid chromatography-tandem mass spectrometry. AU - Cao,Jin, AU - Murch,Susan J, AU - O'Brien,Robert, AU - Saxena,Praveen K, PY - 2006/3/29/received PY - 2006/9/10/revised PY - 2006/9/26/accepted PY - 2006/10/19/pubmed PY - 2007/1/5/medline PY - 2006/10/19/entrez SP - 333 EP - 7 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1134 IS - 1-2 N2 - Currently, the information available on the physiological functions of melatonin in higher plants is rather limited and the role of plant melatonin in human health remains undetermined. Research in this area has been slow due to lack of efficient analytical methods for rapid identification and quantification of the melatonin and related compounds in complex plant matrices. In this communication, we report the development of a rapid, accurate method for extraction, detection and quantification of plant melatonin, serotonin and indole-3-acetic acid (IAA) by Liquid chromatography-tandem mass spectrometry (LC-MS/MS) with electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI), and atmospheric pressure photoionization (APPI), respectively. The limit of detection (LOD) of melatonin in the plant extraction was 5 pg/ml and the limit of quantification (LOQ) was 0.02 ng/ml, as well as LOD for serotonin was 100 pg/ml and the LOQ was 5 ng/ml, LOD for IAA was 50 pg/ml and the LOQ was 0.7 ng/ml. There was a linear relationship between melatonin, serotonin, and IAA concentration and peak area over a quantifiable range of 0.02 ng/ml to 0.1 mg/ml, 5 ng/ml to 0.1 mg/ml, and 0.7 ng/ml to 0.1 mg/ml, respectively, in the plant extract. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/17046004/Rapid_method_for_accurate_analysis_of_melatonin_serotonin_and_auxin_in_plant_samples_using_liquid_chromatography_tandem_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -