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Design and development of a highly stable hydrogen peroxide biosensor on screen printed carbon electrode based on horseradish peroxidase bound with gold nanoparticles in the matrix of chitosan.
Biosens Bioelectron. 2007 Apr 15; 22(9-10):2071-8.BB

Abstract

The design and development of a screen printed carbon electrode (SPCE) on a polyvinyl chloride substrate as a disposable sensor is described. Six configurations were designed on silk screen frames. The SPCEs were printed with four inks: silver ink as the conducting track, carbon ink as the working and counter electrodes, silver/silver chloride ink as the reference electrode and insulating ink as the insulator layer. Selection of the best configuration was done by comparing slopes from the calibration plots generated by the cyclic voltammograms at 10, 20 and 30 mM K(3)Fe(CN)(6) for each configuration. The electrodes with similar configurations gave similar slopes. The 5th configuration was the best electrode that gave the highest slope. Modifying the best SPCE configuration for use as a biosensor, horseradish peroxidase (HRP) was selected as a biomaterial bound with gold nanoparticles (AuNP) in the matrix of chitosan (HRP/AuNP/CHIT). Biosensors of HRP/SPCE, HRP/CHIT/SPCE and HRP/AuNP/CHIT/SPCE were used in the amperometric detection of H(2)O(2) in a solution of 0.1M citrate buffer, pH 6.5, by applying a potential of -0.4V at the working electrode. All the biosensors showed an immediate response to H(2)O(2). The effect of HRP/AuNP incorporated with CHIT (HRP/AuNP/CHIT/SPCE) yielded the highest performance. The amperometric response of HRP/AuNP/CHIT/SPCE retained over 95% of the initial current of the 1st day up to 30 days of storage at 4 degrees C. The biosensor showed a linear range of 0.01-11.3mM H(2)O(2), with a detection limit of 0.65 microM H(2)O(2) (S/N=3). The low detection limit, long storage life and wide linear range of this biosensor make it advantageous in many applications, including bioreactors and biosensors.

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Publisher Full Text

Authors+Show Affiliations

Tangkuaram T
Department of Chemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand. pengtangkua@yahoo.com
Ponchio C
No affiliation info available
Kangkasomboon T
No affiliation info available
Katikawong P
No affiliation info available
Veerasai W
No affiliation info available

MeSH

Biosensing TechniquesCarbonChitosanElectrodesElectroplatingGoldHorseradish PeroxidaseHydrogen PeroxideMetal Nanoparticles

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17046233

Citation

Tangkuaram, Tanin, et al. "Design and Development of a Highly Stable Hydrogen Peroxide Biosensor On Screen Printed Carbon Electrode Based On Horseradish Peroxidase Bound With Gold Nanoparticles in the Matrix of Chitosan." Biosensors & Bioelectronics, vol. 22, no. 9-10, 2007, pp. 2071-8.
Tangkuaram T, Ponchio C, Kangkasomboon T, et al. Design and development of a highly stable hydrogen peroxide biosensor on screen printed carbon electrode based on horseradish peroxidase bound with gold nanoparticles in the matrix of chitosan. Biosens Bioelectron. 2007;22(9-10):2071-8.
Tangkuaram, T., Ponchio, C., Kangkasomboon, T., Katikawong, P., & Veerasai, W. (2007). Design and development of a highly stable hydrogen peroxide biosensor on screen printed carbon electrode based on horseradish peroxidase bound with gold nanoparticles in the matrix of chitosan. Biosensors & Bioelectronics, 22(9-10), 2071-8.
Tangkuaram T, et al. Design and Development of a Highly Stable Hydrogen Peroxide Biosensor On Screen Printed Carbon Electrode Based On Horseradish Peroxidase Bound With Gold Nanoparticles in the Matrix of Chitosan. Biosens Bioelectron. 2007 Apr 15;22(9-10):2071-8. PubMed PMID: 17046233.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Design and development of a highly stable hydrogen peroxide biosensor on screen printed carbon electrode based on horseradish peroxidase bound with gold nanoparticles in the matrix of chitosan. AU - Tangkuaram,Tanin, AU - Ponchio,Chatchai, AU - Kangkasomboon,Thippayawadee, AU - Katikawong,Panadda, AU - Veerasai,Waret, Y1 - 2006/10/13/ PY - 2006/05/02/received PY - 2006/08/17/revised PY - 2006/09/06/accepted PY - 2006/10/19/pubmed PY - 2007/5/26/medline PY - 2006/10/19/entrez SP - 2071 EP - 8 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 22 IS - 9-10 N2 - The design and development of a screen printed carbon electrode (SPCE) on a polyvinyl chloride substrate as a disposable sensor is described. Six configurations were designed on silk screen frames. The SPCEs were printed with four inks: silver ink as the conducting track, carbon ink as the working and counter electrodes, silver/silver chloride ink as the reference electrode and insulating ink as the insulator layer. Selection of the best configuration was done by comparing slopes from the calibration plots generated by the cyclic voltammograms at 10, 20 and 30 mM K(3)Fe(CN)(6) for each configuration. The electrodes with similar configurations gave similar slopes. The 5th configuration was the best electrode that gave the highest slope. Modifying the best SPCE configuration for use as a biosensor, horseradish peroxidase (HRP) was selected as a biomaterial bound with gold nanoparticles (AuNP) in the matrix of chitosan (HRP/AuNP/CHIT). Biosensors of HRP/SPCE, HRP/CHIT/SPCE and HRP/AuNP/CHIT/SPCE were used in the amperometric detection of H(2)O(2) in a solution of 0.1M citrate buffer, pH 6.5, by applying a potential of -0.4V at the working electrode. All the biosensors showed an immediate response to H(2)O(2). The effect of HRP/AuNP incorporated with CHIT (HRP/AuNP/CHIT/SPCE) yielded the highest performance. The amperometric response of HRP/AuNP/CHIT/SPCE retained over 95% of the initial current of the 1st day up to 30 days of storage at 4 degrees C. The biosensor showed a linear range of 0.01-11.3mM H(2)O(2), with a detection limit of 0.65 microM H(2)O(2) (S/N=3). The low detection limit, long storage life and wide linear range of this biosensor make it advantageous in many applications, including bioreactors and biosensors. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/17046233/Design_and_development_of_a_highly_stable_hydrogen_peroxide_biosensor_on_screen_printed_carbon_electrode_based_on_horseradish_peroxidase_bound_with_gold_nanoparticles_in_the_matrix_of_chitosan_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(06)00441-6 DB - PRIME DP - Unbound Medicine ER -