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Molecular characterization of a HMW glutenin subunit allele providing evidence for silencing of x-type gene on Glu-B1.
Yi Chuan Xue Bao. 2006 Oct; 33(10):929-36.YC

Abstract

Understanding the molecular structure of high-molecular-weight glutenin subunit (HMW-GS) may provide useful evidence for the study on the improvement of quality of cultivated wheat and the evolution of Glu-1 alleles. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) shows that the subunits encoded by Glu-B1 were null, named 1Bxm, in a Triticum turgidum var. dicoccoides line PI94640. Primers based on the conserved regions in wheat HMW-GS gene promoter and coding sequences were used to amplify the genomic DNA of line PI94640. The PCR products were sequenced, and the total nucleotide sequence of 3,442 bp including upstream sequence of 1,070 bp was obtained. Compared with the reported gene sequences of Glu-1Bx alleles, the promoter region of the Glu-1Bxm showed close resemblance to 1Bx7. The Glu-1Bxm coding region differs from the other Glu-1Bx alleles for a deduced mature protein with only 212 residues, and a stop codon (TAA) at 637 bp downstream from the start codon was present, which was probably responsible for the silencing of x-type subunit genes at the Glu-B1 locus. Phylogenetic tree based on the nucleotide sequence alignment of HMW glutenin subunit genes showed that 1Bxm was the most ancient type of Glu-B1 alleles, suggesting that the evolution rates are different among Glu-1Bx genes. Further study on the contribution of the unique silenced Glu-B1 alleles to quality improvement was also discussed.

Authors+Show Affiliations

Yang ZJ
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China. yangzujun@uestc.edu.cn
Li GR
No affiliation info available
Liu C
No affiliation info available
Feng J
No affiliation info available
Zhou JP
No affiliation info available
Ren ZL
No affiliation info available

MeSH

AllelesAmino Acid SequenceBase SequenceCloning, MolecularDatabases, GeneticElectrophoresis, Polyacrylamide GelGene SilencingGliadinGlutensMolecular WeightPhylogenyPolymerase Chain ReactionProtein SubunitsSequence AlignmentSequence Analysis, DNATriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17046593

Citation

Yang, Zu-Jun, et al. "Molecular Characterization of a HMW Glutenin Subunit Allele Providing Evidence for Silencing of X-type Gene On Glu-B1." Yi Chuan Xue Bao = Acta Genetica Sinica, vol. 33, no. 10, 2006, pp. 929-36.
Yang ZJ, Li GR, Liu C, et al. Molecular characterization of a HMW glutenin subunit allele providing evidence for silencing of x-type gene on Glu-B1. Yi Chuan Xue Bao. 2006;33(10):929-36.
Yang, Z. J., Li, G. R., Liu, C., Feng, J., Zhou, J. P., & Ren, Z. L. (2006). Molecular characterization of a HMW glutenin subunit allele providing evidence for silencing of x-type gene on Glu-B1. Yi Chuan Xue Bao = Acta Genetica Sinica, 33(10), 929-36.
Yang ZJ, et al. Molecular Characterization of a HMW Glutenin Subunit Allele Providing Evidence for Silencing of X-type Gene On Glu-B1. Yi Chuan Xue Bao. 2006;33(10):929-36. PubMed PMID: 17046593.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of a HMW glutenin subunit allele providing evidence for silencing of x-type gene on Glu-B1. AU - Yang,Zu-Jun, AU - Li,Guang-Rong, AU - Liu,Chang, AU - Feng,Juan, AU - Zhou,Jian-Ping, AU - Ren,Zheng-Long, PY - 2006/06/29/received PY - 2006/09/02/accepted PY - 2006/10/19/pubmed PY - 2009/5/30/medline PY - 2006/10/19/entrez SP - 929 EP - 36 JF - Yi chuan xue bao = Acta genetica Sinica JO - Yi Chuan Xue Bao VL - 33 IS - 10 N2 - Understanding the molecular structure of high-molecular-weight glutenin subunit (HMW-GS) may provide useful evidence for the study on the improvement of quality of cultivated wheat and the evolution of Glu-1 alleles. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) shows that the subunits encoded by Glu-B1 were null, named 1Bxm, in a Triticum turgidum var. dicoccoides line PI94640. Primers based on the conserved regions in wheat HMW-GS gene promoter and coding sequences were used to amplify the genomic DNA of line PI94640. The PCR products were sequenced, and the total nucleotide sequence of 3,442 bp including upstream sequence of 1,070 bp was obtained. Compared with the reported gene sequences of Glu-1Bx alleles, the promoter region of the Glu-1Bxm showed close resemblance to 1Bx7. The Glu-1Bxm coding region differs from the other Glu-1Bx alleles for a deduced mature protein with only 212 residues, and a stop codon (TAA) at 637 bp downstream from the start codon was present, which was probably responsible for the silencing of x-type subunit genes at the Glu-B1 locus. Phylogenetic tree based on the nucleotide sequence alignment of HMW glutenin subunit genes showed that 1Bxm was the most ancient type of Glu-B1 alleles, suggesting that the evolution rates are different among Glu-1Bx genes. Further study on the contribution of the unique silenced Glu-B1 alleles to quality improvement was also discussed. SN - 0379-4172 UR - https://www.unboundmedicine.com/medline/citation/17046593/Molecular_characterization_of_a_HMW_glutenin_subunit_allele_providing_evidence_for_silencing_of_x_type_gene_on_Glu_B1_ DB - PRIME DP - Unbound Medicine ER -