[Determination of akebia saponin D in root of Dipacus asperoides by HPLC].Zhongguo Zhong Yao Za Zhi. 2006 May; 31(9):726-7, 739.ZZ
To develop a quantitative method for the determination of akebia saponin D in root of Dipacus asperoides and provide scientific basis for quality control of D. asperoides.
An IPLC analytical method was established using Kromasil ODS column with acetonitrile-water (30:70) as mobile phase and the detection wavelength was 212 nm.
The akebia saponin D in root extract were well separated by this method. The linear range is between 0.582 5-9.32 microg, r = 0.9999. The average recovery and RSD of repeatability are 100.3%, 2.3%, respectively.
This method is simple, accurate and reliable by evaluating the method validation data and can be used for the quality control of D. asperoides and its preparations.