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[Determination of akebia saponin D in root of Dipacus asperoides by HPLC].
Zhongguo Zhong Yao Za Zhi. 2006 May; 31(9):726-7, 739.ZZ

Abstract

OBJECTIVE

To develop a quantitative method for the determination of akebia saponin D in root of Dipacus asperoides and provide scientific basis for quality control of D. asperoides.

METHOD

An IPLC analytical method was established using Kromasil ODS column with acetonitrile-water (30:70) as mobile phase and the detection wavelength was 212 nm.

RESULT

The akebia saponin D in root extract were well separated by this method. The linear range is between 0.582 5-9.32 microg, r = 0.9999. The average recovery and RSD of repeatability are 100.3%, 2.3%, respectively.

CONCLUSION

This method is simple, accurate and reliable by evaluating the method validation data and can be used for the quality control of D. asperoides and its preparations.

Authors+Show Affiliations

Tan HG
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing.
Lin S
No affiliation info available
Zhang QW
No affiliation info available
Ji L
No affiliation info available

MeSH

ChinaChromatography, High Pressure LiquidDipsacaceaeEcosystemPlant RootsPlants, MedicinalQuality ControlSaponins

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

17048676

Citation

Tan, Hong-gen, et al. "[Determination of Akebia Saponin D in Root of Dipacus Asperoides By HPLC]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 31, no. 9, 2006, pp. 726-7, 739.
Tan HG, Lin S, Zhang QW, et al. [Determination of akebia saponin D in root of Dipacus asperoides by HPLC]. Zhongguo Zhong Yao Za Zhi. 2006;31(9):726-7, 739.
Tan, H. G., Lin, S., Zhang, Q. W., & Ji, L. (2006). [Determination of akebia saponin D in root of Dipacus asperoides by HPLC]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 31(9), 726-7, 739.
Tan HG, et al. [Determination of Akebia Saponin D in Root of Dipacus Asperoides By HPLC]. Zhongguo Zhong Yao Za Zhi. 2006;31(9):726-7, 739. PubMed PMID: 17048676.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Determination of akebia saponin D in root of Dipacus asperoides by HPLC]. AU - Tan,Hong-gen, AU - Lin,Sheng, AU - Zhang,Qi-wei, AU - Ji,Li, PY - 2006/10/20/pubmed PY - 2007/8/4/medline PY - 2006/10/20/entrez SP - 726-7, 739 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 31 IS - 9 N2 - OBJECTIVE: To develop a quantitative method for the determination of akebia saponin D in root of Dipacus asperoides and provide scientific basis for quality control of D. asperoides. METHOD: An IPLC analytical method was established using Kromasil ODS column with acetonitrile-water (30:70) as mobile phase and the detection wavelength was 212 nm. RESULT: The akebia saponin D in root extract were well separated by this method. The linear range is between 0.582 5-9.32 microg, r = 0.9999. The average recovery and RSD of repeatability are 100.3%, 2.3%, respectively. CONCLUSION: This method is simple, accurate and reliable by evaluating the method validation data and can be used for the quality control of D. asperoides and its preparations. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/17048676/[Determination_of_akebia_saponin_D_in_root_of_Dipacus_asperoides_by_HPLC]_ DB - PRIME DP - Unbound Medicine ER -