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Diallyl disulfide suppresses growth of HL-60 cell through increasing histone acetylation and p21WAF1 expression in vivo and in vitro.
Acta Pharmacol Sin 2006; 27(11):1459-66AP

Abstract

AIM

To examine the differentiation induction and growth inhibition of HL-60 cells by diallyl disulfide (DADS), and its relationship with the alterations of histone acetylation and p21(WAF1) expression in vitro and in vivo.

METHODS

Differentiation was studied by nitroblue tetrazolium (NBT) reduction of HL-60 cell in vitro. HL-60 cells 5x10(6) were injected into the right side of the peritoneal cavity of severe combined immunodeficiency (SCID) mice. When the peritoneal neoplasms were detected, the SCID mice were randomly divided into 3 groups and received an ip injection of vehicle alone (NS), DADS or sodium butyrate (SB). The growth inhibition of peritoneal neoplasms induced by DADS was observed by a growth curve. The cycle distribution of HL-60 cells in SCID mice was monitored by flow cytometry. The expression of acetylated histone H3, H4 and p21(WAF1) were measured by Western blot.

RESULTS

After treatment with DADS for 0-72 h, the NBT reduction ability of HL-60 cells increased in a time-dependent manner, compared with no treatment of HL-60 cells. In the HL-60 cells treated with DADS for 24 h, the expression of acetylated histone H3, H4, and p21(WAF1) increased obviously. After treatment with DADS, tumor growth was markedly suppressed. HL-60 cells from mice treated with DADS were blocked in the G1 phase, from 25.4% to 63.4%. The tumors from the mice treated with DADS showed an increase of acetylated histone H3, H4, and p21(WAF1).

CONCLUSION

DADS could induce differentiation and inhibit the growth of HL-60 cells through increasing the expression of acetylated histone H3, H4, and p21(WAF1) in vitro and in vivo.

Authors+Show Affiliations

Cancer Research Institute, Nanhua University, Hengyang 421001, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17049122

Citation

Zhao, Jie, et al. "Diallyl Disulfide Suppresses Growth of HL-60 Cell Through Increasing Histone Acetylation and p21WAF1 Expression in Vivo and in Vitro." Acta Pharmacologica Sinica, vol. 27, no. 11, 2006, pp. 1459-66.
Zhao J, Huang WG, He J, et al. Diallyl disulfide suppresses growth of HL-60 cell through increasing histone acetylation and p21WAF1 expression in vivo and in vitro. Acta Pharmacol Sin. 2006;27(11):1459-66.
Zhao, J., Huang, W. G., He, J., Tan, H., Liao, Q. J., & Su, Q. (2006). Diallyl disulfide suppresses growth of HL-60 cell through increasing histone acetylation and p21WAF1 expression in vivo and in vitro. Acta Pharmacologica Sinica, 27(11), pp. 1459-66.
Zhao J, et al. Diallyl Disulfide Suppresses Growth of HL-60 Cell Through Increasing Histone Acetylation and p21WAF1 Expression in Vivo and in Vitro. Acta Pharmacol Sin. 2006;27(11):1459-66. PubMed PMID: 17049122.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diallyl disulfide suppresses growth of HL-60 cell through increasing histone acetylation and p21WAF1 expression in vivo and in vitro. AU - Zhao,Jie, AU - Huang,Wei-guo, AU - He,Jie, AU - Tan,Hui, AU - Liao,Qian-jin, AU - Su,Qi, PY - 2006/10/20/pubmed PY - 2007/12/12/medline PY - 2006/10/20/entrez SP - 1459 EP - 66 JF - Acta pharmacologica Sinica JO - Acta Pharmacol. Sin. VL - 27 IS - 11 N2 - AIM: To examine the differentiation induction and growth inhibition of HL-60 cells by diallyl disulfide (DADS), and its relationship with the alterations of histone acetylation and p21(WAF1) expression in vitro and in vivo. METHODS: Differentiation was studied by nitroblue tetrazolium (NBT) reduction of HL-60 cell in vitro. HL-60 cells 5x10(6) were injected into the right side of the peritoneal cavity of severe combined immunodeficiency (SCID) mice. When the peritoneal neoplasms were detected, the SCID mice were randomly divided into 3 groups and received an ip injection of vehicle alone (NS), DADS or sodium butyrate (SB). The growth inhibition of peritoneal neoplasms induced by DADS was observed by a growth curve. The cycle distribution of HL-60 cells in SCID mice was monitored by flow cytometry. The expression of acetylated histone H3, H4 and p21(WAF1) were measured by Western blot. RESULTS: After treatment with DADS for 0-72 h, the NBT reduction ability of HL-60 cells increased in a time-dependent manner, compared with no treatment of HL-60 cells. In the HL-60 cells treated with DADS for 24 h, the expression of acetylated histone H3, H4, and p21(WAF1) increased obviously. After treatment with DADS, tumor growth was markedly suppressed. HL-60 cells from mice treated with DADS were blocked in the G1 phase, from 25.4% to 63.4%. The tumors from the mice treated with DADS showed an increase of acetylated histone H3, H4, and p21(WAF1). CONCLUSION: DADS could induce differentiation and inhibit the growth of HL-60 cells through increasing the expression of acetylated histone H3, H4, and p21(WAF1) in vitro and in vivo. SN - 1671-4083 UR - https://www.unboundmedicine.com/medline/citation/17049122/Diallyl_disulfide_suppresses_growth_of_HL_60_cell_through_increasing_histone_acetylation_and_p21WAF1_expression_in_vivo_and_in_vitro_ L2 - http://dx.doi.org/10.1111/j.1745-7254.2006.00433.x DB - PRIME DP - Unbound Medicine ER -