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Antioxidant effect of diphenyl diselenide against sodium nitroprusside (SNP) induced lipid peroxidation in human platelets and erythrocyte membranes: an in vitro evaluation.
Chem Biol Interact. 2006 Dec 01; 164(1-2):126-35.CB

Abstract

An in vitro evaluation on the antioxidant effect of diphenyl diselenide (PhSe)(2), an organochalcogenide, against sodium nitroprusside (SNP)-induced lipid peroxidation (LPO) was conduced. Human platelets and erythrocyte membranes (ghosts), as well as rat brain homogenates (S(1)), were pre-incubated with different concentrations of SNP (0-10 microM). All SNP concentrations tested significantly increased LPO in human platelets and S(1). Platelets were more sensitive to SNP-induced peroxidative damage when compared to S(1). SNP 10 microM decreased glutathione peroxidase (GPx) activity and did not affect glutathione reductase (GR) and catalase (CAT) activities in human platelets. However, ghosts were insensitive to SNP-induced LPO and no changes on GPx, GR and CAT activities were observed. Diphenyl diselenide significantly protected human platelets against SNP-induced LPO and recovered GPx inactivation. This effect was more evident at (PhSe)(2) concentrations above 2 microM. The presented results indicate that (PhSe)(2) exerts protective effects on SNP-induced oxidative damage in human blood components and in rat brain. These phenomena seem to be related to its thiol peroxidase-like activity and to a possible direct interaction with SNP and derivatives. Based on our results and on literature, diphenyl diselenide can be pointed as a promising antioxidant molecule.

Authors+Show Affiliations

Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, 88040-900 Florianópolis, SC, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17049506

Citation

Posser, Thaís, et al. "Antioxidant Effect of Diphenyl Diselenide Against Sodium Nitroprusside (SNP) Induced Lipid Peroxidation in Human Platelets and Erythrocyte Membranes: an in Vitro Evaluation." Chemico-biological Interactions, vol. 164, no. 1-2, 2006, pp. 126-35.
Posser T, Moretto MB, Dafre AL, et al. Antioxidant effect of diphenyl diselenide against sodium nitroprusside (SNP) induced lipid peroxidation in human platelets and erythrocyte membranes: an in vitro evaluation. Chem Biol Interact. 2006;164(1-2):126-35.
Posser, T., Moretto, M. B., Dafre, A. L., Farina, M., da Rocha, J. B., Nogueira, C. W., Zeni, G., Ferreira, J. d. o. s. . S., Leal, R. B., & Franco, J. L. (2006). Antioxidant effect of diphenyl diselenide against sodium nitroprusside (SNP) induced lipid peroxidation in human platelets and erythrocyte membranes: an in vitro evaluation. Chemico-biological Interactions, 164(1-2), 126-35.
Posser T, et al. Antioxidant Effect of Diphenyl Diselenide Against Sodium Nitroprusside (SNP) Induced Lipid Peroxidation in Human Platelets and Erythrocyte Membranes: an in Vitro Evaluation. Chem Biol Interact. 2006 Dec 1;164(1-2):126-35. PubMed PMID: 17049506.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant effect of diphenyl diselenide against sodium nitroprusside (SNP) induced lipid peroxidation in human platelets and erythrocyte membranes: an in vitro evaluation. AU - Posser,Thaís, AU - Moretto,Maria Beatriz, AU - Dafre,Alcir Luiz, AU - Farina,Marcelo, AU - da Rocha,João Batista Teixeira, AU - Nogueira,Cristina Wayne, AU - Zeni,Gilson, AU - Ferreira,Jovino Dos Santos, AU - Leal,Rodrigo Bainy, AU - Franco,Jeferson Luis, Y1 - 2006/09/12/ PY - 2006/03/21/received PY - 2006/09/04/revised PY - 2006/09/05/accepted PY - 2006/10/20/pubmed PY - 2007/1/19/medline PY - 2006/10/20/entrez SP - 126 EP - 35 JF - Chemico-biological interactions JO - Chem Biol Interact VL - 164 IS - 1-2 N2 - An in vitro evaluation on the antioxidant effect of diphenyl diselenide (PhSe)(2), an organochalcogenide, against sodium nitroprusside (SNP)-induced lipid peroxidation (LPO) was conduced. Human platelets and erythrocyte membranes (ghosts), as well as rat brain homogenates (S(1)), were pre-incubated with different concentrations of SNP (0-10 microM). All SNP concentrations tested significantly increased LPO in human platelets and S(1). Platelets were more sensitive to SNP-induced peroxidative damage when compared to S(1). SNP 10 microM decreased glutathione peroxidase (GPx) activity and did not affect glutathione reductase (GR) and catalase (CAT) activities in human platelets. However, ghosts were insensitive to SNP-induced LPO and no changes on GPx, GR and CAT activities were observed. Diphenyl diselenide significantly protected human platelets against SNP-induced LPO and recovered GPx inactivation. This effect was more evident at (PhSe)(2) concentrations above 2 microM. The presented results indicate that (PhSe)(2) exerts protective effects on SNP-induced oxidative damage in human blood components and in rat brain. These phenomena seem to be related to its thiol peroxidase-like activity and to a possible direct interaction with SNP and derivatives. Based on our results and on literature, diphenyl diselenide can be pointed as a promising antioxidant molecule. SN - 0009-2797 UR - https://www.unboundmedicine.com/medline/citation/17049506/Antioxidant_effect_of_diphenyl_diselenide_against_sodium_nitroprusside__SNP__induced_lipid_peroxidation_in_human_platelets_and_erythrocyte_membranes:_an_in_vitro_evaluation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0009-2797(06)00249-3 DB - PRIME DP - Unbound Medicine ER -