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Identification and immunogenicity of an immunodominant mimotope of Avibacterium paragallinarum from a phage display peptide library.

Abstract

Avibacterium paragallinarum is the causative agent of infectious coryza. The protective antigens of this important pathogen have not yet been clearly identified. In this paper, we applied phage display technique to screen the immunodominant mimotopes of a serovar A strain of A. paragallinarum by using a random 12-peptide library, and evaluated the immunogenicity in chickens of the selected mimotope. Polyclonal antibody directed against A. paragallinarum strain 0083 (serovar A) was used as the target antibody and phage clones binding to this target were screened from the 12-mer random peptide library. More than 50% of the phage clones selected in the third round carried the consensus peptide motif sequence A-DP(M)L. The phage clones containing the peptide motif reacted with the target antibody and this interaction could be blocked, in a dose-dependent manner, by A. paragallinarum. One of the peptide sequences, YGLLAVDPLFKP, was selected and the corresponding oligonucleotide sequence was synthesized and then inserted into the expression vector pFliTrx. The recombinant plasmid was transferred into an expression host Escherichia coli GI826 by electroporation, resulting in a recombinant E. coli expressing the peptide on the bacterial surface. Intramuscular injection of the epitope-expressing recombinant bacteria into chickens induced a specific serological response to serovar A. A. paragallinarum. The chickens given the recombinant E. coli showed significant protection against challenge with A. paragallinarum 0083. These results indicated a potential for the use of the mimotope in the development of molecular vaccines for infectious coryza.

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  • Authors+Show Affiliations

    ,

    Key Laboratory of Preventive Veterinary Medicine of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100094, China.

    , , , , , , ,

    Source

    Veterinary microbiology 119:2-4 2007 Jan 31 pg 231-9

    MeSH

    Amino Acid Sequence
    Animals
    Antibodies, Bacterial
    Antigens, Bacterial
    Bacteriophages
    Base Sequence
    Chickens
    DNA, Bacterial
    Dose-Response Relationship, Immunologic
    Enzyme-Linked Immunosorbent Assay
    Female
    Haemophilus Infections
    Haemophilus paragallinarum
    Immunodominant Epitopes
    Molecular Sequence Data
    Peptide Library
    Peptides
    Poultry Diseases
    Respiratory Tract Infections
    Specific Pathogen-Free Organisms

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    17049758

    Citation

    Wang, Hongjun, et al. "Identification and Immunogenicity of an Immunodominant Mimotope of Avibacterium Paragallinarum From a Phage Display Peptide Library." Veterinary Microbiology, vol. 119, no. 2-4, 2007, pp. 231-9.
    Wang H, Gao Y, Gong Y, et al. Identification and immunogenicity of an immunodominant mimotope of Avibacterium paragallinarum from a phage display peptide library. Vet Microbiol. 2007;119(2-4):231-9.
    Wang, H., Gao, Y., Gong, Y., Chen, X., Liu, C., Zhou, X., ... Yang, H. (2007). Identification and immunogenicity of an immunodominant mimotope of Avibacterium paragallinarum from a phage display peptide library. Veterinary Microbiology, 119(2-4), pp. 231-9.
    Wang H, et al. Identification and Immunogenicity of an Immunodominant Mimotope of Avibacterium Paragallinarum From a Phage Display Peptide Library. Vet Microbiol. 2007 Jan 31;119(2-4):231-9. PubMed PMID: 17049758.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Identification and immunogenicity of an immunodominant mimotope of Avibacterium paragallinarum from a phage display peptide library. AU - Wang,Hongjun, AU - Gao,Yaping, AU - Gong,Yumei, AU - Chen,Xiaoling, AU - Liu,Chuan, AU - Zhou,Xuemei, AU - Blackall,P J, AU - Zhang,Peijun, AU - Yang,Hanchun, Y1 - 2006/09/17/ PY - 2006/07/10/received PY - 2006/08/26/revised PY - 2006/08/31/accepted PY - 2006/10/20/pubmed PY - 2007/11/1/medline PY - 2006/10/20/entrez SP - 231 EP - 9 JF - Veterinary microbiology JO - Vet. Microbiol. VL - 119 IS - 2-4 N2 - Avibacterium paragallinarum is the causative agent of infectious coryza. The protective antigens of this important pathogen have not yet been clearly identified. In this paper, we applied phage display technique to screen the immunodominant mimotopes of a serovar A strain of A. paragallinarum by using a random 12-peptide library, and evaluated the immunogenicity in chickens of the selected mimotope. Polyclonal antibody directed against A. paragallinarum strain 0083 (serovar A) was used as the target antibody and phage clones binding to this target were screened from the 12-mer random peptide library. More than 50% of the phage clones selected in the third round carried the consensus peptide motif sequence A-DP(M)L. The phage clones containing the peptide motif reacted with the target antibody and this interaction could be blocked, in a dose-dependent manner, by A. paragallinarum. One of the peptide sequences, YGLLAVDPLFKP, was selected and the corresponding oligonucleotide sequence was synthesized and then inserted into the expression vector pFliTrx. The recombinant plasmid was transferred into an expression host Escherichia coli GI826 by electroporation, resulting in a recombinant E. coli expressing the peptide on the bacterial surface. Intramuscular injection of the epitope-expressing recombinant bacteria into chickens induced a specific serological response to serovar A. A. paragallinarum. The chickens given the recombinant E. coli showed significant protection against challenge with A. paragallinarum 0083. These results indicated a potential for the use of the mimotope in the development of molecular vaccines for infectious coryza. SN - 0378-1135 UR - https://www.unboundmedicine.com/medline/citation/17049758/Identification_and_immunogenicity_of_an_immunodominant_mimotope_of_Avibacterium_paragallinarum_from_a_phage_display_peptide_library_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(06)00351-8 DB - PRIME DP - Unbound Medicine ER -