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Identification and immunogenicity of an immunodominant mimotope of Avibacterium paragallinarum from a phage display peptide library.
Vet Microbiol 2007; 119(2-4):231-9VM

Abstract

Avibacterium paragallinarum is the causative agent of infectious coryza. The protective antigens of this important pathogen have not yet been clearly identified. In this paper, we applied phage display technique to screen the immunodominant mimotopes of a serovar A strain of A. paragallinarum by using a random 12-peptide library, and evaluated the immunogenicity in chickens of the selected mimotope. Polyclonal antibody directed against A. paragallinarum strain 0083 (serovar A) was used as the target antibody and phage clones binding to this target were screened from the 12-mer random peptide library. More than 50% of the phage clones selected in the third round carried the consensus peptide motif sequence A-DP(M)L. The phage clones containing the peptide motif reacted with the target antibody and this interaction could be blocked, in a dose-dependent manner, by A. paragallinarum. One of the peptide sequences, YGLLAVDPLFKP, was selected and the corresponding oligonucleotide sequence was synthesized and then inserted into the expression vector pFliTrx. The recombinant plasmid was transferred into an expression host Escherichia coli GI826 by electroporation, resulting in a recombinant E. coli expressing the peptide on the bacterial surface. Intramuscular injection of the epitope-expressing recombinant bacteria into chickens induced a specific serological response to serovar A. A. paragallinarum. The chickens given the recombinant E. coli showed significant protection against challenge with A. paragallinarum 0083. These results indicated a potential for the use of the mimotope in the development of molecular vaccines for infectious coryza.

Authors+Show Affiliations

Key Laboratory of Preventive Veterinary Medicine of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100094, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17049758

Citation

Wang, Hongjun, et al. "Identification and Immunogenicity of an Immunodominant Mimotope of Avibacterium Paragallinarum From a Phage Display Peptide Library." Veterinary Microbiology, vol. 119, no. 2-4, 2007, pp. 231-9.
Wang H, Gao Y, Gong Y, et al. Identification and immunogenicity of an immunodominant mimotope of Avibacterium paragallinarum from a phage display peptide library. Vet Microbiol. 2007;119(2-4):231-9.
Wang, H., Gao, Y., Gong, Y., Chen, X., Liu, C., Zhou, X., ... Yang, H. (2007). Identification and immunogenicity of an immunodominant mimotope of Avibacterium paragallinarum from a phage display peptide library. Veterinary Microbiology, 119(2-4), pp. 231-9.
Wang H, et al. Identification and Immunogenicity of an Immunodominant Mimotope of Avibacterium Paragallinarum From a Phage Display Peptide Library. Vet Microbiol. 2007 Jan 31;119(2-4):231-9. PubMed PMID: 17049758.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and immunogenicity of an immunodominant mimotope of Avibacterium paragallinarum from a phage display peptide library. AU - Wang,Hongjun, AU - Gao,Yaping, AU - Gong,Yumei, AU - Chen,Xiaoling, AU - Liu,Chuan, AU - Zhou,Xuemei, AU - Blackall,P J, AU - Zhang,Peijun, AU - Yang,Hanchun, Y1 - 2006/09/17/ PY - 2006/07/10/received PY - 2006/08/26/revised PY - 2006/08/31/accepted PY - 2006/10/20/pubmed PY - 2007/11/1/medline PY - 2006/10/20/entrez SP - 231 EP - 9 JF - Veterinary microbiology JO - Vet. Microbiol. VL - 119 IS - 2-4 N2 - Avibacterium paragallinarum is the causative agent of infectious coryza. The protective antigens of this important pathogen have not yet been clearly identified. In this paper, we applied phage display technique to screen the immunodominant mimotopes of a serovar A strain of A. paragallinarum by using a random 12-peptide library, and evaluated the immunogenicity in chickens of the selected mimotope. Polyclonal antibody directed against A. paragallinarum strain 0083 (serovar A) was used as the target antibody and phage clones binding to this target were screened from the 12-mer random peptide library. More than 50% of the phage clones selected in the third round carried the consensus peptide motif sequence A-DP(M)L. The phage clones containing the peptide motif reacted with the target antibody and this interaction could be blocked, in a dose-dependent manner, by A. paragallinarum. One of the peptide sequences, YGLLAVDPLFKP, was selected and the corresponding oligonucleotide sequence was synthesized and then inserted into the expression vector pFliTrx. The recombinant plasmid was transferred into an expression host Escherichia coli GI826 by electroporation, resulting in a recombinant E. coli expressing the peptide on the bacterial surface. Intramuscular injection of the epitope-expressing recombinant bacteria into chickens induced a specific serological response to serovar A. A. paragallinarum. The chickens given the recombinant E. coli showed significant protection against challenge with A. paragallinarum 0083. These results indicated a potential for the use of the mimotope in the development of molecular vaccines for infectious coryza. SN - 0378-1135 UR - https://www.unboundmedicine.com/medline/citation/17049758/Identification_and_immunogenicity_of_an_immunodominant_mimotope_of_Avibacterium_paragallinarum_from_a_phage_display_peptide_library_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(06)00351-8 DB - PRIME DP - Unbound Medicine ER -