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Characterization of an influenza A H5N2 reassortant as a candidate for live-attenuated and inactivated vaccines against highly pathogenic H5N1 viruses with pandemic potential.
Vaccine. 2006 Nov 17; 24(47-48):6859-66.V

Abstract

We generated a high-growth 7:1 reassortant (Len17/H5) that contained the hemagglutinin (HA) gene from non-pathogenic A/Duck/Potsdam/1402-6/86 (H5N2) virus and other genes from the cold-adapted (ca) attenuated A/Leningrad/134/17/57 (H2H2) strain. Len17/H5 demonstrated an attenuated phenotype in mice and did not infect chickens. Mice administered Len17/H5 either as a live-attenuated intranasal vaccine or as an inactivated intramuscular vaccine were substantially protected from lethal challenge with highly pathogenic A/Hong Kong/483/97 (H5N1) virus and were protected from pulmonary infection with antigenically distinct A/Hong Kong/213/2003 (H5N1) virus. The cross-protective effect correlated with the levels of virus-specific mucosal IgA and/or serum IgG antibodies. Our results suggest a new strategy of using classical genetic reassortment between a high-growth ca H2N2 strain and antigenically related non-pathogenic avian viruses to prepare live-attenuated and inactivated vaccines for influenza pandemic.

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Authors+Show Affiliations

Desheva JA
Department of Virology, Institute of Experimental Medicine, RAMS, St Petersburg, Russia.
Lu XH
No affiliation info available
Rekstin AR
No affiliation info available
Rudenko LG
No affiliation info available
Swayne DE
No affiliation info available
Cox NJ
No affiliation info available
Katz JM
No affiliation info available
Klimov AI
No affiliation info available

MeSH

AnimalsAntibodies, ViralChickensCross ReactionsDisease OutbreaksFemaleHumansImmunizationInfluenza A Virus, H5N1 SubtypeInfluenza A Virus, H5N2 SubtypeInfluenza VaccinesInfluenza in BirdsInfluenza, HumanMiceMice, Inbred BALB CNeutralization TestsOrthomyxoviridae InfectionsVaccines, AttenuatedVaccines, InactivatedVirus Replication

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17050041

Citation

Desheva, J A., et al. "Characterization of an Influenza a H5N2 Reassortant as a Candidate for Live-attenuated and Inactivated Vaccines Against Highly Pathogenic H5N1 Viruses With Pandemic Potential." Vaccine, vol. 24, no. 47-48, 2006, pp. 6859-66.
Desheva JA, Lu XH, Rekstin AR, et al. Characterization of an influenza A H5N2 reassortant as a candidate for live-attenuated and inactivated vaccines against highly pathogenic H5N1 viruses with pandemic potential. Vaccine. 2006;24(47-48):6859-66.
Desheva, J. A., Lu, X. H., Rekstin, A. R., Rudenko, L. G., Swayne, D. E., Cox, N. J., Katz, J. M., & Klimov, A. I. (2006). Characterization of an influenza A H5N2 reassortant as a candidate for live-attenuated and inactivated vaccines against highly pathogenic H5N1 viruses with pandemic potential. Vaccine, 24(47-48), 6859-66.
Desheva JA, et al. Characterization of an Influenza a H5N2 Reassortant as a Candidate for Live-attenuated and Inactivated Vaccines Against Highly Pathogenic H5N1 Viruses With Pandemic Potential. Vaccine. 2006 Nov 17;24(47-48):6859-66. PubMed PMID: 17050041.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of an influenza A H5N2 reassortant as a candidate for live-attenuated and inactivated vaccines against highly pathogenic H5N1 viruses with pandemic potential. AU - Desheva,J A, AU - Lu,X H, AU - Rekstin,A R, AU - Rudenko,L G, AU - Swayne,D E, AU - Cox,N J, AU - Katz,J M, AU - Klimov,A I, Y1 - 2006/06/28/ PY - 2006/04/02/received PY - 2006/05/31/revised PY - 2006/06/14/accepted PY - 2006/10/20/pubmed PY - 2007/1/5/medline PY - 2006/10/20/entrez SP - 6859 EP - 66 JF - Vaccine JO - Vaccine VL - 24 IS - 47-48 N2 - We generated a high-growth 7:1 reassortant (Len17/H5) that contained the hemagglutinin (HA) gene from non-pathogenic A/Duck/Potsdam/1402-6/86 (H5N2) virus and other genes from the cold-adapted (ca) attenuated A/Leningrad/134/17/57 (H2H2) strain. Len17/H5 demonstrated an attenuated phenotype in mice and did not infect chickens. Mice administered Len17/H5 either as a live-attenuated intranasal vaccine or as an inactivated intramuscular vaccine were substantially protected from lethal challenge with highly pathogenic A/Hong Kong/483/97 (H5N1) virus and were protected from pulmonary infection with antigenically distinct A/Hong Kong/213/2003 (H5N1) virus. The cross-protective effect correlated with the levels of virus-specific mucosal IgA and/or serum IgG antibodies. Our results suggest a new strategy of using classical genetic reassortment between a high-growth ca H2N2 strain and antigenically related non-pathogenic avian viruses to prepare live-attenuated and inactivated vaccines for influenza pandemic. SN - 0264-410X UR - https://www.unboundmedicine.com/medline/citation/17050041/Characterization_of_an_influenza_A_H5N2_reassortant_as_a_candidate_for_live_attenuated_and_inactivated_vaccines_against_highly_pathogenic_H5N1_viruses_with_pandemic_potential_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0264-410X(06)00742-0 DB - PRIME DP - Unbound Medicine ER -