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Expression of HB-EGF by retinal pigment epithelial cells in vitreoretinal proliferative disease.
Curr Eye Res. 2006 Oct; 31(10):863-74.CE

Abstract

The heparin-binding epidermal growth factor-like growth factor (HB-EGF) has been implicated in wound-healing processes of various tissues. However, it is not known whether HB-EGF may represent a factor implicated in overstimulated wound-healing processes of the retina during proliferative retinopathies. Therefore, we investigated whether human retinal pigment epithelial (RPE) cells, which are crucially involved in proliferative retinopathies, express and respond to HB-EGF. RPE cells express mRNAs for various members of the EGF-related growth factor family, among them for HB-EGF, as well as for the EGF receptors ErbB1, -2, -3, and -4. The gene expression of HB-EGF is stimulated in the presence of transforming and basic fibroblast growth factors and by oxidative stress and is suppressed during chemical hypoxia. Exogenous HB-EGF stimulates proliferation and migration of RPE cells and the gene and protein expression of the vascular endothelial growth factor (VEGF). HB-EGF activates at least three signal transduction pathways in RPE cells including the extracellular signal-regulated kinases (involved in the proliferation-stimulating action of HB-EGF), p38 (mediates the effects on chemotaxis and secretion of VEGF), and the phosphatidylinositol-3 kinase (necessary for the stimulation of chemotaxis). In epiretinal membranes of patients with proliferative retinopathies, HB-EGF immunoreactivity was partially colocalized with the RPE cell marker, cytokeratins; this observation suggests that RPE cell-derived HB-EGF may represent one factor that drives the uncontrolled wound-healing process of the retina. The stimulating effect on the secretion of VEGF may suggest that HB-EGF is also implicated in the pathological angiogenesis of the retina.

Authors+Show Affiliations

Department of Ophthalmology and Eye Clinic, University of Leipzig, Leipzig, Germany. hollbm@medizin.uni-leipzig.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17050278

Citation

Hollborn, Margrit, et al. "Expression of HB-EGF By Retinal Pigment Epithelial Cells in Vitreoretinal Proliferative Disease." Current Eye Research, vol. 31, no. 10, 2006, pp. 863-74.
Hollborn M, Iandiev I, Seifert M, et al. Expression of HB-EGF by retinal pigment epithelial cells in vitreoretinal proliferative disease. Curr Eye Res. 2006;31(10):863-74.
Hollborn, M., Iandiev, I., Seifert, M., Schnurrbusch, U. E., Wolf, S., Wiedemann, P., Bringmann, A., & Kohen, L. (2006). Expression of HB-EGF by retinal pigment epithelial cells in vitreoretinal proliferative disease. Current Eye Research, 31(10), 863-74.
Hollborn M, et al. Expression of HB-EGF By Retinal Pigment Epithelial Cells in Vitreoretinal Proliferative Disease. Curr Eye Res. 2006;31(10):863-74. PubMed PMID: 17050278.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of HB-EGF by retinal pigment epithelial cells in vitreoretinal proliferative disease. AU - Hollborn,Margrit, AU - Iandiev,Ianors, AU - Seifert,Marlen, AU - Schnurrbusch,Ute E K, AU - Wolf,Sebastian, AU - Wiedemann,Peter, AU - Bringmann,Andreas, AU - Kohen,Leon, PY - 2006/10/20/pubmed PY - 2006/11/15/medline PY - 2006/10/20/entrez SP - 863 EP - 74 JF - Current eye research JO - Curr. Eye Res. VL - 31 IS - 10 N2 - The heparin-binding epidermal growth factor-like growth factor (HB-EGF) has been implicated in wound-healing processes of various tissues. However, it is not known whether HB-EGF may represent a factor implicated in overstimulated wound-healing processes of the retina during proliferative retinopathies. Therefore, we investigated whether human retinal pigment epithelial (RPE) cells, which are crucially involved in proliferative retinopathies, express and respond to HB-EGF. RPE cells express mRNAs for various members of the EGF-related growth factor family, among them for HB-EGF, as well as for the EGF receptors ErbB1, -2, -3, and -4. The gene expression of HB-EGF is stimulated in the presence of transforming and basic fibroblast growth factors and by oxidative stress and is suppressed during chemical hypoxia. Exogenous HB-EGF stimulates proliferation and migration of RPE cells and the gene and protein expression of the vascular endothelial growth factor (VEGF). HB-EGF activates at least three signal transduction pathways in RPE cells including the extracellular signal-regulated kinases (involved in the proliferation-stimulating action of HB-EGF), p38 (mediates the effects on chemotaxis and secretion of VEGF), and the phosphatidylinositol-3 kinase (necessary for the stimulation of chemotaxis). In epiretinal membranes of patients with proliferative retinopathies, HB-EGF immunoreactivity was partially colocalized with the RPE cell marker, cytokeratins; this observation suggests that RPE cell-derived HB-EGF may represent one factor that drives the uncontrolled wound-healing process of the retina. The stimulating effect on the secretion of VEGF may suggest that HB-EGF is also implicated in the pathological angiogenesis of the retina. SN - 0271-3683 UR - https://www.unboundmedicine.com/medline/citation/17050278/Expression_of_HB_EGF_by_retinal_pigment_epithelial_cells_in_vitreoretinal_proliferative_disease_ L2 - http://www.tandfonline.com/doi/full/10.1080/02713680600888807 DB - PRIME DP - Unbound Medicine ER -