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Upregulated liver conversion of alpha-linolenic acid to docosahexaenoic acid in rats on a 15 week n-3 PUFA-deficient diet.
J Lipid Res. 2007 Jan; 48(1):152-64.JL

Abstract

We quantified incorporation rates of plasma-derived alpha-linolenic acid (alpha-LNA, 18:3n-3) into "stable" liver lipids and the conversion rate of alpha-LNA to docosahexaenoic acid (DHA, 22:6n-3) in male rats fed, after weaning, an n-3 PUFA-adequate diet (4.6% alpha-LNA, no DHA) or an n-3 PUFA-deficient diet (0.2% alpha-LNA, no DHA) for 15 weeks. Unanesthetized rats were infused intravenously with [1-14C]alpha-LNA, and arterial plasma was sampled until the liver was microwaved at 5 min. Unlabeled alpha-LNA and DHA concentrations in arterial plasma and liver were reduced >90% by deprivation, whereas unlabeled arachidonic acid (20:4n-6) and docosapentaenoic acid (22:5n-6) concentrations were increased. Deprivation did not change alpha-LNA incorporation coefficients into stable liver lipids but increased synthesis-incorporation coefficients of DHA from alpha-LNA by 6.6-, 8.4-, and 2.3-fold in triacylglycerol, phospholipid, and cholesteryl ester, respectively. Assuming that synthesized-incorporated DHA eventually would be secreted within lipoproteins, calculated liver DHA secretion rates equaled 2.19 and 0.82 micromol/day in the n-3 PUFA-adequate and -deprived rats, respectively. These rates exceed the published rates of brain DHA consumption by 6- and 10-fold, respectively, and should be sufficient to maintain normal and reduced brain DHA concentrations, respectively, in the two dietary conditions.

Authors+Show Affiliations

Brain Physiology and Metabolism Section, National Institute on Aging, National Institutes of Health, Bethesda, MD 20892, USA. mikii@mail.nih.govNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Intramural

Language

eng

PubMed ID

17050905

Citation

Igarashi, Miki, et al. "Upregulated Liver Conversion of Alpha-linolenic Acid to Docosahexaenoic Acid in Rats On a 15 Week N-3 PUFA-deficient Diet." Journal of Lipid Research, vol. 48, no. 1, 2007, pp. 152-64.
Igarashi M, DeMar JC, Ma K, et al. Upregulated liver conversion of alpha-linolenic acid to docosahexaenoic acid in rats on a 15 week n-3 PUFA-deficient diet. J Lipid Res. 2007;48(1):152-64.
Igarashi, M., DeMar, J. C., Ma, K., Chang, L., Bell, J. M., & Rapoport, S. I. (2007). Upregulated liver conversion of alpha-linolenic acid to docosahexaenoic acid in rats on a 15 week n-3 PUFA-deficient diet. Journal of Lipid Research, 48(1), 152-64.
Igarashi M, et al. Upregulated Liver Conversion of Alpha-linolenic Acid to Docosahexaenoic Acid in Rats On a 15 Week N-3 PUFA-deficient Diet. J Lipid Res. 2007;48(1):152-64. PubMed PMID: 17050905.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Upregulated liver conversion of alpha-linolenic acid to docosahexaenoic acid in rats on a 15 week n-3 PUFA-deficient diet. AU - Igarashi,Miki, AU - DeMar,James C,Jr AU - Ma,Kaizong, AU - Chang,Lisa, AU - Bell,Jane M, AU - Rapoport,Stanley I, Y1 - 2006/10/18/ PY - 2006/10/20/pubmed PY - 2007/7/17/medline PY - 2006/10/20/entrez SP - 152 EP - 64 JF - Journal of lipid research JO - J Lipid Res VL - 48 IS - 1 N2 - We quantified incorporation rates of plasma-derived alpha-linolenic acid (alpha-LNA, 18:3n-3) into "stable" liver lipids and the conversion rate of alpha-LNA to docosahexaenoic acid (DHA, 22:6n-3) in male rats fed, after weaning, an n-3 PUFA-adequate diet (4.6% alpha-LNA, no DHA) or an n-3 PUFA-deficient diet (0.2% alpha-LNA, no DHA) for 15 weeks. Unanesthetized rats were infused intravenously with [1-14C]alpha-LNA, and arterial plasma was sampled until the liver was microwaved at 5 min. Unlabeled alpha-LNA and DHA concentrations in arterial plasma and liver were reduced >90% by deprivation, whereas unlabeled arachidonic acid (20:4n-6) and docosapentaenoic acid (22:5n-6) concentrations were increased. Deprivation did not change alpha-LNA incorporation coefficients into stable liver lipids but increased synthesis-incorporation coefficients of DHA from alpha-LNA by 6.6-, 8.4-, and 2.3-fold in triacylglycerol, phospholipid, and cholesteryl ester, respectively. Assuming that synthesized-incorporated DHA eventually would be secreted within lipoproteins, calculated liver DHA secretion rates equaled 2.19 and 0.82 micromol/day in the n-3 PUFA-adequate and -deprived rats, respectively. These rates exceed the published rates of brain DHA consumption by 6- and 10-fold, respectively, and should be sufficient to maintain normal and reduced brain DHA concentrations, respectively, in the two dietary conditions. SN - 0022-2275 UR - https://www.unboundmedicine.com/medline/citation/17050905/Upregulated_liver_conversion_of_alpha_linolenic_acid_to_docosahexaenoic_acid_in_rats_on_a_15_week_n_3_PUFA_deficient_diet_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2275(20)43646-6 DB - PRIME DP - Unbound Medicine ER -