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SAGE analysis of mosquito salivary gland transcriptomes during Plasmodium invasion.
Cell Microbiol. 2007 Mar; 9(3):708-24.CM

Abstract

Invasion of the vector salivary glands by Plasmodium is a critical step for malaria transmission. To describe salivary gland cellular responses to sporozoite invasion, we have undertaken the analysis of Anopheles gambiae salivary gland transcriptome using Serial Analysis of Gene Expression (SAGE). Statistical analysis of the more than 160000 sequenced tags generated from four libraries, two from glands infected by Plasmodium berghei, two from glands of controls, revealed that at least 57 Anopheles genes are differentially expressed in infected salivary glands. Among the 37 immune-related genes identified by SAGE tags, four (Defensin1, GNBP, Serpin6 and Cecropin2) were found to be upregulated during salivary gland invasion, while five genes encoding small secreted proteins display induction patterns strongly reminiscent of that of Cecropin2. Invasion by Plasmodium has also an impact on the expression of genes involved in transport, lipid and energy metabolism, suggesting that the sporozoite may exploit the metabolism of its host. In contrast, protein composition of saliva is predicted to be only slightly modified after infection. This study, which is the first transcriptome analysis of the salivary gland response to Plasmodium infection, provides a basis for a better understanding of Plasmodium/Anopheles salivary gland interactions.

Authors+Show Affiliations

Unité de Biochimie et Biologie Moléculaire des Insectes, Institut Pasteur, 28 rue du Dr Roux, 75724, Paris, France. ichupin@pasteur.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17054438

Citation

Rosinski-Chupin, Isabelle, et al. "SAGE Analysis of Mosquito Salivary Gland Transcriptomes During Plasmodium Invasion." Cellular Microbiology, vol. 9, no. 3, 2007, pp. 708-24.
Rosinski-Chupin I, Briolay J, Brouilly P, et al. SAGE analysis of mosquito salivary gland transcriptomes during Plasmodium invasion. Cell Microbiol. 2007;9(3):708-24.
Rosinski-Chupin, I., Briolay, J., Brouilly, P., Perrot, S., Gomez, S. M., Chertemps, T., Roth, C. W., Keime, C., Gandrillon, O., Couble, P., & Brey, P. T. (2007). SAGE analysis of mosquito salivary gland transcriptomes during Plasmodium invasion. Cellular Microbiology, 9(3), 708-24.
Rosinski-Chupin I, et al. SAGE Analysis of Mosquito Salivary Gland Transcriptomes During Plasmodium Invasion. Cell Microbiol. 2007;9(3):708-24. PubMed PMID: 17054438.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SAGE analysis of mosquito salivary gland transcriptomes during Plasmodium invasion. AU - Rosinski-Chupin,Isabelle, AU - Briolay,Jérôme, AU - Brouilly,Patrick, AU - Perrot,Sylvie, AU - Gomez,Shawn M, AU - Chertemps,Thomas, AU - Roth,Charles W, AU - Keime,Céline, AU - Gandrillon,Olivier, AU - Couble,Pierre, AU - Brey,Paul T, Y1 - 2006/10/18/ PY - 2006/10/24/pubmed PY - 2007/6/27/medline PY - 2006/10/24/entrez SP - 708 EP - 24 JF - Cellular microbiology JO - Cell. Microbiol. VL - 9 IS - 3 N2 - Invasion of the vector salivary glands by Plasmodium is a critical step for malaria transmission. To describe salivary gland cellular responses to sporozoite invasion, we have undertaken the analysis of Anopheles gambiae salivary gland transcriptome using Serial Analysis of Gene Expression (SAGE). Statistical analysis of the more than 160000 sequenced tags generated from four libraries, two from glands infected by Plasmodium berghei, two from glands of controls, revealed that at least 57 Anopheles genes are differentially expressed in infected salivary glands. Among the 37 immune-related genes identified by SAGE tags, four (Defensin1, GNBP, Serpin6 and Cecropin2) were found to be upregulated during salivary gland invasion, while five genes encoding small secreted proteins display induction patterns strongly reminiscent of that of Cecropin2. Invasion by Plasmodium has also an impact on the expression of genes involved in transport, lipid and energy metabolism, suggesting that the sporozoite may exploit the metabolism of its host. In contrast, protein composition of saliva is predicted to be only slightly modified after infection. This study, which is the first transcriptome analysis of the salivary gland response to Plasmodium infection, provides a basis for a better understanding of Plasmodium/Anopheles salivary gland interactions. SN - 1462-5814 UR - https://www.unboundmedicine.com/medline/citation/17054438/SAGE_analysis_of_mosquito_salivary_gland_transcriptomes_during_Plasmodium_invasion_ L2 - https://doi.org/10.1111/j.1462-5822.2006.00822.x DB - PRIME DP - Unbound Medicine ER -