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Human skeletal muscle fibre type variations correlate with PPAR alpha, PPAR delta and PGC-1 alpha mRNA.
Acta Physiol (Oxf). 2006 Nov-Dec; 188(3-4):207-16.AP

Abstract

AIMS

Studies from genetically modified animals have been instrumental in highlighting genes and their products involved in the regulation of muscle fibre type and oxidative phenotypes; however, evidence in humans is limited. Our aim was therefore to investigate expression of those genes implicated in the regulation of oxidative fibre phenotypes in humans.

METHODS

Using quantitative polymerase chain reaction we determined mRNA expression of selected genes in skeletal muscle from three different groups, displaying physiological and pathological variations in muscle fibre type, activity and skeletal muscle metabolism respectively: (i) elite athletes (cyclists), with an increased proportion of type I slow twitch, oxidative fibres, (ii) normally active subjects with an average fibre type distribution, and (iii) spinal cord-injured subjects with a low proportion of type I fibres.

RESULTS

Skeletal muscle mRNA expression of calcineurin Aalpha and Abeta, peroxisome proliferator-activated receptor (PPAR)-alpha and -delta, and PPAR gamma coactivator (PGC)-1alpha and -1beta was determined. Calcineurin Aalpha and calcineurin Abeta mRNA expression was similar between groups. In contrast, mRNA expression of PPARalpha, PPARdelta, PGC-1alpha and -1beta was increased in athletes, when compared with normally active subjects. Furthermore, mRNA expression of PPARalpha, PPARdelta, PGC-1alpha and -1beta was reduced in spinal cord-injured subjects. Additionally, PPARalpha, PPARdelta and PGC-1alpha correlated with oxidative fibre content.

CONCLUSION

Skeletal muscle mRNA expression of PPARalpha, PPARdelta, PGC-1alpha and -1beta reflects differences in type I muscle fibres associated with pathologically and physiologically induced skeletal muscle fibre type differences.

Authors+Show Affiliations

Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17054660

Citation

Krämer, D K., et al. "Human Skeletal Muscle Fibre Type Variations Correlate With PPAR Alpha, PPAR Delta and PGC-1 Alpha MRNA." Acta Physiologica (Oxford, England), vol. 188, no. 3-4, 2006, pp. 207-16.
Krämer DK, Ahlsén M, Norrbom J, et al. Human skeletal muscle fibre type variations correlate with PPAR alpha, PPAR delta and PGC-1 alpha mRNA. Acta Physiol (Oxf). 2006;188(3-4):207-16.
Krämer, D. K., Ahlsén, M., Norrbom, J., Jansson, E., Hjeltnes, N., Gustafsson, T., & Krook, A. (2006). Human skeletal muscle fibre type variations correlate with PPAR alpha, PPAR delta and PGC-1 alpha mRNA. Acta Physiologica (Oxford, England), 188(3-4), 207-16.
Krämer DK, et al. Human Skeletal Muscle Fibre Type Variations Correlate With PPAR Alpha, PPAR Delta and PGC-1 Alpha MRNA. Acta Physiol (Oxf). 2006;188(3-4):207-16. PubMed PMID: 17054660.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human skeletal muscle fibre type variations correlate with PPAR alpha, PPAR delta and PGC-1 alpha mRNA. AU - Krämer,D K, AU - Ahlsén,M, AU - Norrbom,J, AU - Jansson,E, AU - Hjeltnes,N, AU - Gustafsson,T, AU - Krook,A, PY - 2006/10/24/pubmed PY - 2007/2/13/medline PY - 2006/10/24/entrez SP - 207 EP - 16 JF - Acta physiologica (Oxford, England) JO - Acta Physiol (Oxf) VL - 188 IS - 3-4 N2 - AIMS: Studies from genetically modified animals have been instrumental in highlighting genes and their products involved in the regulation of muscle fibre type and oxidative phenotypes; however, evidence in humans is limited. Our aim was therefore to investigate expression of those genes implicated in the regulation of oxidative fibre phenotypes in humans. METHODS: Using quantitative polymerase chain reaction we determined mRNA expression of selected genes in skeletal muscle from three different groups, displaying physiological and pathological variations in muscle fibre type, activity and skeletal muscle metabolism respectively: (i) elite athletes (cyclists), with an increased proportion of type I slow twitch, oxidative fibres, (ii) normally active subjects with an average fibre type distribution, and (iii) spinal cord-injured subjects with a low proportion of type I fibres. RESULTS: Skeletal muscle mRNA expression of calcineurin Aalpha and Abeta, peroxisome proliferator-activated receptor (PPAR)-alpha and -delta, and PPAR gamma coactivator (PGC)-1alpha and -1beta was determined. Calcineurin Aalpha and calcineurin Abeta mRNA expression was similar between groups. In contrast, mRNA expression of PPARalpha, PPARdelta, PGC-1alpha and -1beta was increased in athletes, when compared with normally active subjects. Furthermore, mRNA expression of PPARalpha, PPARdelta, PGC-1alpha and -1beta was reduced in spinal cord-injured subjects. Additionally, PPARalpha, PPARdelta and PGC-1alpha correlated with oxidative fibre content. CONCLUSION: Skeletal muscle mRNA expression of PPARalpha, PPARdelta, PGC-1alpha and -1beta reflects differences in type I muscle fibres associated with pathologically and physiologically induced skeletal muscle fibre type differences. SN - 1748-1708 UR - https://www.unboundmedicine.com/medline/citation/17054660/Human_skeletal_muscle_fibre_type_variations_correlate_with_PPAR_alpha_PPAR_delta_and_PGC_1_alpha_mRNA_ L2 - https://doi.org/10.1111/j.1748-1716.2006.01620.x DB - PRIME DP - Unbound Medicine ER -