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[Establishment of RP-HPLC fingerprint for quality assessment of Cortex Periplocae].
Zhong Yao Cai. 2006 Jul; 29(7):715-7.ZY

Abstract

OBJECTIVE

To establish the RP-HPLC fingerprint analysis for the quality control of Cortex Periplocae.

METHODS

HPLC fingerprint and analysis method of Cortex Periplocae were studied. Kromasil C18 column (250 mm x 4.6 mm, 5 microm) was used, with mixture of acetonitrile and 0.025% phosphate solution as mobile phase in a gradient mode. The flow rate was 1.0 ml/min. The wavelength of measurement was 220 nm. 15 Batches of Cortex Periplocae were determined.

RESULTS

The 15 samples were classified as 4 clusters by cluster analysis and the 6 superior in producing area samples were confirmed to establish the mutual model. The samples' quality was assessed by "Similarity Evaluation System for Chromatographic Fingerprint of TCM 2004".

CONCLUSION

The method is simple and reliable. It can be used to evaluate and control the quality of Cortex Periplocae conveniently.

Authors+Show Affiliations

Li DY
School of Pharmacy, Shenyang Pharmaceutical University, China.
Wang TT
No affiliation info available
Chen XH
No affiliation info available
Bi KS
No affiliation info available

MeSH

Chromatography, High Pressure LiquidCluster AnalysisDrug ContaminationDrugs, Chinese HerbalPeriplocaPlant BarkPlant RootsPlants, MedicinalQuality ControlReproducibility of Results

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

17059012

Citation

Li, Dan-yi, et al. "[Establishment of RP-HPLC Fingerprint for Quality Assessment of Cortex Periplocae]." Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, vol. 29, no. 7, 2006, pp. 715-7.
Li DY, Wang TT, Chen XH, et al. [Establishment of RP-HPLC fingerprint for quality assessment of Cortex Periplocae]. Zhong Yao Cai. 2006;29(7):715-7.
Li, D. Y., Wang, T. T., Chen, X. H., & Bi, K. S. (2006). [Establishment of RP-HPLC fingerprint for quality assessment of Cortex Periplocae]. Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, 29(7), 715-7.
Li DY, et al. [Establishment of RP-HPLC Fingerprint for Quality Assessment of Cortex Periplocae]. Zhong Yao Cai. 2006;29(7):715-7. PubMed PMID: 17059012.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Establishment of RP-HPLC fingerprint for quality assessment of Cortex Periplocae]. AU - Li,Dan-yi, AU - Wang,Ting-ting, AU - Chen,Xiao-hui, AU - Bi,Kai-shun, PY - 2006/10/25/pubmed PY - 2007/5/10/medline PY - 2006/10/25/entrez SP - 715 EP - 7 JF - Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials JO - Zhong Yao Cai VL - 29 IS - 7 N2 - OBJECTIVE: To establish the RP-HPLC fingerprint analysis for the quality control of Cortex Periplocae. METHODS: HPLC fingerprint and analysis method of Cortex Periplocae were studied. Kromasil C18 column (250 mm x 4.6 mm, 5 microm) was used, with mixture of acetonitrile and 0.025% phosphate solution as mobile phase in a gradient mode. The flow rate was 1.0 ml/min. The wavelength of measurement was 220 nm. 15 Batches of Cortex Periplocae were determined. RESULTS: The 15 samples were classified as 4 clusters by cluster analysis and the 6 superior in producing area samples were confirmed to establish the mutual model. The samples' quality was assessed by "Similarity Evaluation System for Chromatographic Fingerprint of TCM 2004". CONCLUSION: The method is simple and reliable. It can be used to evaluate and control the quality of Cortex Periplocae conveniently. SN - 1001-4454 UR - https://www.unboundmedicine.com/medline/citation/17059012/[Establishment_of_RP_HPLC_fingerprint_for_quality_assessment_of_Cortex_Periplocae]_ DB - PRIME DP - Unbound Medicine ER -