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Over-expression of OsAGAP, an ARF-GAP, interferes with auxin influx, vesicle trafficking and root development.
Plant J. 2006 Nov; 48(4):581-91.PJ

Abstract

Development and organogenesis in both dicot and monocot plants are highly dependent on polar auxin transport (PAT), which requires the proper asymmetric localization of both auxin influx and efflux carriers. In the model dicot plant Arabidopsis thaliana, the trafficking and localization of auxin efflux facilitators such as PIN-FORMED1 (PIN1) are mediated by GNOM, a guanine-nucleotide exchange factor (GEF) for the ADP-ribosylation factor (ARF) family of small GTPases, but molecular regulators of the auxin influx facilitators remain unknown. Here, we show that over-expression of OsAGAP, an ARF-GTPase-activating protein (ARF-GAP) in rice, impaired PAT and interfered with both primary and lateral root development. The lateral root phenotype could be rescued by the membrane-permeable auxin 1-naphthyl acetic acid, but not by indole 3-acetic acid (IAA) or by 2,4-dichloro-phenoxyacetic acid, which require influx facilitators to enter the cells. OsAGAP-over-expressing plants had alterations in vesicle trafficking and localization of the presumptive A. thaliana auxin-influx carrier AUX1, but not in the localization of the auxin efflux facilitators. Together, our data suggest that OsAGAP has a specific role in regulating vesicle trafficking pathways such as the auxin influx pathway, which in turn controls auxin-dependent root growth in plants.

Authors+Show Affiliations

Key Laboratory of Photosynthesis and Molecular Environmental Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17059407

Citation

Zhuang, Xiaolei, et al. "Over-expression of OsAGAP, an ARF-GAP, Interferes With Auxin Influx, Vesicle Trafficking and Root Development." The Plant Journal : for Cell and Molecular Biology, vol. 48, no. 4, 2006, pp. 581-91.
Zhuang X, Jiang J, Li J, et al. Over-expression of OsAGAP, an ARF-GAP, interferes with auxin influx, vesicle trafficking and root development. Plant J. 2006;48(4):581-91.
Zhuang, X., Jiang, J., Li, J., Ma, Q., Xu, Y., Xue, Y., Xu, Z., & Chong, K. (2006). Over-expression of OsAGAP, an ARF-GAP, interferes with auxin influx, vesicle trafficking and root development. The Plant Journal : for Cell and Molecular Biology, 48(4), 581-91.
Zhuang X, et al. Over-expression of OsAGAP, an ARF-GAP, Interferes With Auxin Influx, Vesicle Trafficking and Root Development. Plant J. 2006;48(4):581-91. PubMed PMID: 17059407.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Over-expression of OsAGAP, an ARF-GAP, interferes with auxin influx, vesicle trafficking and root development. AU - Zhuang,Xiaolei, AU - Jiang,Jiafu, AU - Li,Junhua, AU - Ma,Qibin, AU - Xu,Yunyuan, AU - Xue,Yongbiao, AU - Xu,Zhihong, AU - Chong,Kang, Y1 - 2006/10/19/ PY - 2006/10/25/pubmed PY - 2007/3/14/medline PY - 2006/10/25/entrez SP - 581 EP - 91 JF - The Plant journal : for cell and molecular biology JO - Plant J VL - 48 IS - 4 N2 - Development and organogenesis in both dicot and monocot plants are highly dependent on polar auxin transport (PAT), which requires the proper asymmetric localization of both auxin influx and efflux carriers. In the model dicot plant Arabidopsis thaliana, the trafficking and localization of auxin efflux facilitators such as PIN-FORMED1 (PIN1) are mediated by GNOM, a guanine-nucleotide exchange factor (GEF) for the ADP-ribosylation factor (ARF) family of small GTPases, but molecular regulators of the auxin influx facilitators remain unknown. Here, we show that over-expression of OsAGAP, an ARF-GTPase-activating protein (ARF-GAP) in rice, impaired PAT and interfered with both primary and lateral root development. The lateral root phenotype could be rescued by the membrane-permeable auxin 1-naphthyl acetic acid, but not by indole 3-acetic acid (IAA) or by 2,4-dichloro-phenoxyacetic acid, which require influx facilitators to enter the cells. OsAGAP-over-expressing plants had alterations in vesicle trafficking and localization of the presumptive A. thaliana auxin-influx carrier AUX1, but not in the localization of the auxin efflux facilitators. Together, our data suggest that OsAGAP has a specific role in regulating vesicle trafficking pathways such as the auxin influx pathway, which in turn controls auxin-dependent root growth in plants. SN - 0960-7412 UR - https://www.unboundmedicine.com/medline/citation/17059407/Over_expression_of_OsAGAP_an_ARF_GAP_interferes_with_auxin_influx_vesicle_trafficking_and_root_development_ DB - PRIME DP - Unbound Medicine ER -