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The Arabidopsis MAP kinase kinase MKK1 participates in defence responses to the bacterial elicitor flagellin.
Plant J. 2006 Nov; 48(4):485-98.PJ

Abstract

Plants sense pathogens through both pathogen-associated molecular patterns and recognition of race-specific virulence factors, which induce basal defence or an accelerated defence (often manifest in the form of local cell death), respectively. A mitogen-activated protein kinase (MAPK) module in Arabidopsis was previously proposed to signal from perception of the bacterial elicitor flagellin to the activation of basal defence-related genes. Here, we present evidence for a parallel MAPK-signalling pathway involved in the response to flg22, a peptide corresponding to the most conserved domain of flagellin. The endogenous Arabidopsis MAP kinase kinase MKK1 is activated in cells treated with flg22, phosphorylates the MAPK MPK4 in vitro, and activates it in vivo in protoplasts. In mkk1 mutant plants, the activation by flg22 of MPK4 and two other flg22-induced MAPKs (MPK3 and MPK6) is impaired. In the mkk1 mutant, a battery of both flg22-induced and flg22-repressed genes show altered expression, indicating that MKK1 negatively regulates the activity of flagellin-responsive genes. Intriguingly, in contrast to the mpk4 mutant, mkk1 shows no morphological anomalies and is compromised in resistance to both virulent and avirulent Pseudomonas syringae strains. Thus, the MKK1 signalling pathway modulates the expression of genes responding to elicitors and plays an important role in pathogen defence.

Authors+Show Affiliations

School of Biological Sciences, Royal Holloway University of London, Egham TW20 0EX, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17059410

Citation

Mészáros, Tamás, et al. "The Arabidopsis MAP Kinase Kinase MKK1 Participates in Defence Responses to the Bacterial Elicitor Flagellin." The Plant Journal : for Cell and Molecular Biology, vol. 48, no. 4, 2006, pp. 485-98.
Mészáros T, Helfer A, Hatzimasoura E, et al. The Arabidopsis MAP kinase kinase MKK1 participates in defence responses to the bacterial elicitor flagellin. Plant J. 2006;48(4):485-98.
Mészáros, T., Helfer, A., Hatzimasoura, E., Magyar, Z., Serazetdinova, L., Rios, G., Bardóczy, V., Teige, M., Koncz, C., Peck, S., & Bögre, L. (2006). The Arabidopsis MAP kinase kinase MKK1 participates in defence responses to the bacterial elicitor flagellin. The Plant Journal : for Cell and Molecular Biology, 48(4), 485-98.
Mészáros T, et al. The Arabidopsis MAP Kinase Kinase MKK1 Participates in Defence Responses to the Bacterial Elicitor Flagellin. Plant J. 2006;48(4):485-98. PubMed PMID: 17059410.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Arabidopsis MAP kinase kinase MKK1 participates in defence responses to the bacterial elicitor flagellin. AU - Mészáros,Tamás, AU - Helfer,Anne, AU - Hatzimasoura,Elizabeth, AU - Magyar,Zoltán, AU - Serazetdinova,Liliya, AU - Rios,Gabino, AU - Bardóczy,Viola, AU - Teige,Markus, AU - Koncz,Csaba, AU - Peck,Scott, AU - Bögre,László, Y1 - 2006/10/20/ PY - 2006/10/25/pubmed PY - 2007/3/14/medline PY - 2006/10/25/entrez SP - 485 EP - 98 JF - The Plant journal : for cell and molecular biology JO - Plant J. VL - 48 IS - 4 N2 - Plants sense pathogens through both pathogen-associated molecular patterns and recognition of race-specific virulence factors, which induce basal defence or an accelerated defence (often manifest in the form of local cell death), respectively. A mitogen-activated protein kinase (MAPK) module in Arabidopsis was previously proposed to signal from perception of the bacterial elicitor flagellin to the activation of basal defence-related genes. Here, we present evidence for a parallel MAPK-signalling pathway involved in the response to flg22, a peptide corresponding to the most conserved domain of flagellin. The endogenous Arabidopsis MAP kinase kinase MKK1 is activated in cells treated with flg22, phosphorylates the MAPK MPK4 in vitro, and activates it in vivo in protoplasts. In mkk1 mutant plants, the activation by flg22 of MPK4 and two other flg22-induced MAPKs (MPK3 and MPK6) is impaired. In the mkk1 mutant, a battery of both flg22-induced and flg22-repressed genes show altered expression, indicating that MKK1 negatively regulates the activity of flagellin-responsive genes. Intriguingly, in contrast to the mpk4 mutant, mkk1 shows no morphological anomalies and is compromised in resistance to both virulent and avirulent Pseudomonas syringae strains. Thus, the MKK1 signalling pathway modulates the expression of genes responding to elicitors and plays an important role in pathogen defence. SN - 0960-7412 UR - https://www.unboundmedicine.com/medline/citation/17059410/The_Arabidopsis_MAP_kinase_kinase_MKK1_participates_in_defence_responses_to_the_bacterial_elicitor_flagellin_ L2 - https://doi.org/10.1111/j.1365-313X.2006.02888.x DB - PRIME DP - Unbound Medicine ER -