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[Qualitative fingerprint and quantitative determination of caffeic acid in compound dandelion enema].
Nan Fang Yi Ke Da Xue Xue Bao. 2006 Oct; 26(10):1503-5.NF

Abstract

OBJECTIVE

To establish a qualitative and quantitative reversed-phase high-performance liquid chromatography (RP-HPLC) with fingerprinting technique for quality control of compound dandelion enema.

METHODS

HPLC was utilized for quality assessment of 10 batches of samples. RP-HPLC analysis was performed on a Hypersil BDS C18 column (4.6 mm x 250 mm, 5 microm) with the mixture of acetonitrile (A) and potassium phosphate solution (B) (pH3.2) as the mobile phase in gradient mode. The concentrations of solvent A were 10%, 80% and 80% at 0, 38 and 40 min, respectively. The column temperature was set at 35 degrees C, the flow rate at 0.7 ml/min and the detection wavelength at 254 nm.

RESULTS

HPLC fingerprinting was established from the 10 batches, and the data showed 23 characteristic peaks in the compound dandelion enema for use as index peaks for qualitative identification. Comparison of the retention time and the on-line UV spectra of the samples with the chemical standards identified peaks 3, 4 and 8 as protocatechualdehyde, caffeic acid and ferulic acid, respectively. The contents of caffeic acid in the compound dandelion enema ranged between 63.7 and 136.8 microg/ml.

CONCLUSION

High specific chromatographic fingerprinting and quantitative measurement of caffeic acid allows rigorous quality control of compound dandelion enema.

Authors+Show Affiliations

Li YL
Department of Pharmacy, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Yan Y
No affiliation info available
Tan YF
No affiliation info available
Xu ZY
No affiliation info available
Chen ZL
No affiliation info available

MeSH

Caffeic AcidsChromatography, High Pressure LiquidDrugs, Chinese HerbalReference StandardsReproducibility of ResultsTaraxacum

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

17062364

Citation

Li, Yi-lei, et al. "[Qualitative Fingerprint and Quantitative Determination of Caffeic Acid in Compound Dandelion Enema]." Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, vol. 26, no. 10, 2006, pp. 1503-5.
Li YL, Yan Y, Tan YF, et al. [Qualitative fingerprint and quantitative determination of caffeic acid in compound dandelion enema]. Nan Fang Yi Ke Da Xue Xue Bao. 2006;26(10):1503-5.
Li, Y. L., Yan, Y., Tan, Y. F., Xu, Z. Y., & Chen, Z. L. (2006). [Qualitative fingerprint and quantitative determination of caffeic acid in compound dandelion enema]. Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, 26(10), 1503-5.
Li YL, et al. [Qualitative Fingerprint and Quantitative Determination of Caffeic Acid in Compound Dandelion Enema]. Nan Fang Yi Ke Da Xue Xue Bao. 2006;26(10):1503-5. PubMed PMID: 17062364.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Qualitative fingerprint and quantitative determination of caffeic acid in compound dandelion enema]. AU - Li,Yi-lei, AU - Yan,Yuan, AU - Tan,Ya-fei, AU - Xu,Zhong-yuan, AU - Chen,Zhi-liang, PY - 2006/10/26/pubmed PY - 2008/4/25/medline PY - 2006/10/26/entrez SP - 1503 EP - 5 JF - Nan fang yi ke da xue xue bao = Journal of Southern Medical University JO - Nan Fang Yi Ke Da Xue Xue Bao VL - 26 IS - 10 N2 - OBJECTIVE: To establish a qualitative and quantitative reversed-phase high-performance liquid chromatography (RP-HPLC) with fingerprinting technique for quality control of compound dandelion enema. METHODS: HPLC was utilized for quality assessment of 10 batches of samples. RP-HPLC analysis was performed on a Hypersil BDS C18 column (4.6 mm x 250 mm, 5 microm) with the mixture of acetonitrile (A) and potassium phosphate solution (B) (pH3.2) as the mobile phase in gradient mode. The concentrations of solvent A were 10%, 80% and 80% at 0, 38 and 40 min, respectively. The column temperature was set at 35 degrees C, the flow rate at 0.7 ml/min and the detection wavelength at 254 nm. RESULTS: HPLC fingerprinting was established from the 10 batches, and the data showed 23 characteristic peaks in the compound dandelion enema for use as index peaks for qualitative identification. Comparison of the retention time and the on-line UV spectra of the samples with the chemical standards identified peaks 3, 4 and 8 as protocatechualdehyde, caffeic acid and ferulic acid, respectively. The contents of caffeic acid in the compound dandelion enema ranged between 63.7 and 136.8 microg/ml. CONCLUSION: High specific chromatographic fingerprinting and quantitative measurement of caffeic acid allows rigorous quality control of compound dandelion enema. SN - 1673-4254 UR - https://www.unboundmedicine.com/medline/citation/17062364/[Qualitative_fingerprint_and_quantitative_determination_of_caffeic_acid_in_compound_dandelion_enema]_ DB - PRIME DP - Unbound Medicine ER -