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Evaluation of calcofluor white stain for detection of Pneumocystis carinii.
Diagn Microbiol Infect Dis. 1990 Jul-Aug; 13(4):307-10.DM

Abstract

A rapid calcofluor white (CFW) stain for detecting Pneumocystis carinii was evaluated prospectively. Eighty-nine bronchoalveolar lavage (BAL) specimens, 21 open-lung biopsy (OLB) tissues, 2 induced sputums, 1 expectorated sputum, 2 tracheal secretions, and 1 bronchial secretion from 102 patients were examined for P. carinii cysts by both the CFW stain and a modified methenamine silver (MS) stain. Twenty episodes of P. carinii pneumonia were detected: 19 of these episodes were detected by CFW stain, and 16 of those episodes were detected by MS stain. Discrepancies between the two staining methods were resolved by review of the clinical histories and, in one case, by testing an OLB specimen. Neither staining procedure gave false-positive results with any specimen. More cysts were detected in CFW-stained specimens than in MS-stained specimens (p = 0.05). CFW stain is a simple, rapid, and inexpensive method for detecting P. carinii in clinical specimens and is at least as sensitive as MS stain.

Authors+Show Affiliations

Department of Laboratory Medicine, Mayo Clinic, Rochester, Minnesota 55905.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

1706242

Citation

Kim, Y K., et al. "Evaluation of Calcofluor White Stain for Detection of Pneumocystis Carinii." Diagnostic Microbiology and Infectious Disease, vol. 13, no. 4, 1990, pp. 307-10.
Kim YK, Parulekar S, Yu PK, et al. Evaluation of calcofluor white stain for detection of Pneumocystis carinii. Diagn Microbiol Infect Dis. 1990;13(4):307-10.
Kim, Y. K., Parulekar, S., Yu, P. K., Pisani, R. J., Smith, T. F., & Anhalt, J. P. (1990). Evaluation of calcofluor white stain for detection of Pneumocystis carinii. Diagnostic Microbiology and Infectious Disease, 13(4), 307-10.
Kim YK, et al. Evaluation of Calcofluor White Stain for Detection of Pneumocystis Carinii. Diagn Microbiol Infect Dis. 1990 Jul-Aug;13(4):307-10. PubMed PMID: 1706242.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of calcofluor white stain for detection of Pneumocystis carinii. AU - Kim,Y K, AU - Parulekar,S, AU - Yu,P K, AU - Pisani,R J, AU - Smith,T F, AU - Anhalt,J P, PY - 1990/7/1/pubmed PY - 1990/7/1/medline PY - 1990/7/1/entrez SP - 307 EP - 10 JF - Diagnostic microbiology and infectious disease JO - Diagn Microbiol Infect Dis VL - 13 IS - 4 N2 - A rapid calcofluor white (CFW) stain for detecting Pneumocystis carinii was evaluated prospectively. Eighty-nine bronchoalveolar lavage (BAL) specimens, 21 open-lung biopsy (OLB) tissues, 2 induced sputums, 1 expectorated sputum, 2 tracheal secretions, and 1 bronchial secretion from 102 patients were examined for P. carinii cysts by both the CFW stain and a modified methenamine silver (MS) stain. Twenty episodes of P. carinii pneumonia were detected: 19 of these episodes were detected by CFW stain, and 16 of those episodes were detected by MS stain. Discrepancies between the two staining methods were resolved by review of the clinical histories and, in one case, by testing an OLB specimen. Neither staining procedure gave false-positive results with any specimen. More cysts were detected in CFW-stained specimens than in MS-stained specimens (p = 0.05). CFW stain is a simple, rapid, and inexpensive method for detecting P. carinii in clinical specimens and is at least as sensitive as MS stain. SN - 0732-8893 UR - https://www.unboundmedicine.com/medline/citation/1706242/Evaluation_of_calcofluor_white_stain_for_detection_of_Pneumocystis_carinii_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0732-8893(90)90022-N DB - PRIME DP - Unbound Medicine ER -