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Expression of aromatase, estrogen receptor alpha and beta, androgen receptor, and cytochrome P-450scc in the human early prepubertal testis.
Pediatr Res. 2006 Dec; 60(6):740-4.PR

Abstract

The expression of aromatase, estrogen receptor alpha (ERalpha) and beta (ERbeta), androgen receptor (AR), and cytochrome P-450 side chain cleavage enzyme (cP450scc) was studied in prepubertal testis. Samples were divided in three age groups (GRs): GR1, newborns (1- to 21-d-old neonates, n = 5); GR2, postnatal activation stage (1- to 7-mo-old infants, n = 6); GR3, childhood (12- to 60-mo-old boys, n = 4). Absent or very poor detection of ERalpha by immunohistochemistry in all cells and by mRNA expression was observed. Leydig cells (LCs) of GR1 and GR2 showed strong immunostaining of aromatase and cP450scc but weak staining of ERbeta and AR. Interstitial cells (ICs) and Sertoli cells (SCs) expressed ERbeta, particularly in GR1 and GR2. Strong expression of AR was found in peritubular cells (PCs). For all markers, expression in GR3 was the weakest. In germ cells (GCs), i.e. gonocytes and spermatogonia, aromatase and ERbeta were immunoexpressed strongly whereas no expression of ERalpha, AR, or cP450scc was detected. It is proposed that in newborn and infantile testis, testosterone acting on PCs might modulate infant LC differentiation, whereas the absence of AR in SCs prevents development of spermatogenesis. The role of estrogen is less clear, but it could modulate the preservation of an adequate pool of precursor LCs and GCs.

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Authors+Show Affiliations

Berensztein EB
Research Laboratory, Hospital de Pediatria Garrahan, Buenos Aires C124 5AAM, Argentina.
Baquedano MS
No affiliation info available
Gonzalez CR
No affiliation info available
Saraco NI
No affiliation info available
Rodriguez J
No affiliation info available
Ponzio R
No affiliation info available
Rivarola MA
No affiliation info available
Belgorosky A
No affiliation info available

MeSH

AromataseCell DifferentiationChild, PreschoolCholesterol Side-Chain Cleavage EnzymeEstrogen Receptor alphaEstrogen Receptor betaGene Expression RegulationGene Expression Regulation, EnzymologicGerm CellsHumansInfantInfant, NewbornLeydig CellsMaleRNA, MessengerReceptors, AndrogenSertoli CellsTestis

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17065579

Citation

Berensztein, Esperanza B., et al. "Expression of Aromatase, Estrogen Receptor Alpha and Beta, Androgen Receptor, and Cytochrome P-450scc in the Human Early Prepubertal Testis." Pediatric Research, vol. 60, no. 6, 2006, pp. 740-4.
Berensztein EB, Baquedano MS, Gonzalez CR, et al. Expression of aromatase, estrogen receptor alpha and beta, androgen receptor, and cytochrome P-450scc in the human early prepubertal testis. Pediatr Res. 2006;60(6):740-4.
Berensztein, E. B., Baquedano, M. S., Gonzalez, C. R., Saraco, N. I., Rodriguez, J., Ponzio, R., Rivarola, M. A., & Belgorosky, A. (2006). Expression of aromatase, estrogen receptor alpha and beta, androgen receptor, and cytochrome P-450scc in the human early prepubertal testis. Pediatric Research, 60(6), 740-4.
Berensztein EB, et al. Expression of Aromatase, Estrogen Receptor Alpha and Beta, Androgen Receptor, and Cytochrome P-450scc in the Human Early Prepubertal Testis. Pediatr Res. 2006;60(6):740-4. PubMed PMID: 17065579.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of aromatase, estrogen receptor alpha and beta, androgen receptor, and cytochrome P-450scc in the human early prepubertal testis. AU - Berensztein,Esperanza B, AU - Baquedano,María Sonia, AU - Gonzalez,Candela R, AU - Saraco,Nora I, AU - Rodriguez,Jorge, AU - Ponzio,Roberto, AU - Rivarola,Marco A, AU - Belgorosky,Alicia, Y1 - 2006/10/25/ PY - 2006/10/27/pubmed PY - 2007/1/19/medline PY - 2006/10/27/entrez SP - 740 EP - 4 JF - Pediatric research JO - Pediatr Res VL - 60 IS - 6 N2 - The expression of aromatase, estrogen receptor alpha (ERalpha) and beta (ERbeta), androgen receptor (AR), and cytochrome P-450 side chain cleavage enzyme (cP450scc) was studied in prepubertal testis. Samples were divided in three age groups (GRs): GR1, newborns (1- to 21-d-old neonates, n = 5); GR2, postnatal activation stage (1- to 7-mo-old infants, n = 6); GR3, childhood (12- to 60-mo-old boys, n = 4). Absent or very poor detection of ERalpha by immunohistochemistry in all cells and by mRNA expression was observed. Leydig cells (LCs) of GR1 and GR2 showed strong immunostaining of aromatase and cP450scc but weak staining of ERbeta and AR. Interstitial cells (ICs) and Sertoli cells (SCs) expressed ERbeta, particularly in GR1 and GR2. Strong expression of AR was found in peritubular cells (PCs). For all markers, expression in GR3 was the weakest. In germ cells (GCs), i.e. gonocytes and spermatogonia, aromatase and ERbeta were immunoexpressed strongly whereas no expression of ERalpha, AR, or cP450scc was detected. It is proposed that in newborn and infantile testis, testosterone acting on PCs might modulate infant LC differentiation, whereas the absence of AR in SCs prevents development of spermatogenesis. The role of estrogen is less clear, but it could modulate the preservation of an adequate pool of precursor LCs and GCs. SN - 0031-3998 UR - https://www.unboundmedicine.com/medline/citation/17065579/Expression_of_aromatase_estrogen_receptor_alpha_and_beta_androgen_receptor_and_cytochrome_P_450scc_in_the_human_early_prepubertal_testis_ L2 - https://doi.org/10.1203/01.pdr.0000246072.04663.bb DB - PRIME DP - Unbound Medicine ER -
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