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Multiple pathways for cationic amino acid transport in rat seminiferous tubule cells.
Biol Reprod. 2007 Feb; 76(2):241-9.BR

Abstract

Arginine and ornithine are known to be important for various biological processes in the testis, but the delivery of extracellular cationic amino acids to the seminiferous tubule cells remains poorly understood. We investigated the activity and expression of cationic amino acid transporters in isolated rat Sertoli cells, peritubular cells, pachytene spermatocytes, and early spermatids. We assessed the l-arginine uptake kinetics, Na(+) dependence of transport, profiles of cis inhibition of uptake by cationic and neutral amino acids, and sensitivity to trans stimulation of cationic amino acid transporters, and studied the expression of the genes encoding them by RT-PCR. Our data suggest that l-arginine is taken up by Sertoli cells and peritubular cells, principally via system y(+)L (SLC3A2/SLC7A6) and system y(+) (SLC7A1 and SLC7A2), with system B(0+) making a minor contribution. By contrast, system B(0+), associated with system y(+)L (SLC3A2/SLC7A7 and SLC7A6), made a major contribution to the transport of cationic amino acids in pachytene spermatocytes and early spermatids. Sertoli cells had higher rates of l-arginine transport than the other seminiferous tubule cells. This high efficiency of arginine transport in Sertoli cells and the properties of the y(+)L system predominating in these cells strongly suggest that Sertoli cells play a key role in supplying germ cells with l-arginine and other cationic amino acids. Furthermore, whereas cytokines induce nitric oxide (NO) production in peritubular and Sertoli cells, little or no upregulation of arginine transport by cytokines was observed in these cells. Thus, NO synthesis does not depend on the stimulation of arginine transport in these somatic tubular cells.

Authors+Show Affiliations

Inserm, U625, Rennes F-35042, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17065601

Citation

Cérec, Virginie, et al. "Multiple Pathways for Cationic Amino Acid Transport in Rat Seminiferous Tubule Cells." Biology of Reproduction, vol. 76, no. 2, 2007, pp. 241-9.
Cérec V, Piquet-Pellorce C, Aly HA, et al. Multiple pathways for cationic amino acid transport in rat seminiferous tubule cells. Biol Reprod. 2007;76(2):241-9.
Cérec, V., Piquet-Pellorce, C., Aly, H. A., Touzalin, A. M., Jégou, B., & Bauché, F. (2007). Multiple pathways for cationic amino acid transport in rat seminiferous tubule cells. Biology of Reproduction, 76(2), 241-9.
Cérec V, et al. Multiple Pathways for Cationic Amino Acid Transport in Rat Seminiferous Tubule Cells. Biol Reprod. 2007;76(2):241-9. PubMed PMID: 17065601.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multiple pathways for cationic amino acid transport in rat seminiferous tubule cells. AU - Cérec,Virginie, AU - Piquet-Pellorce,Claire, AU - Aly,Hamdy A A, AU - Touzalin,Anne-Marie, AU - Jégou,Bernard, AU - Bauché,Françoise, Y1 - 2006/10/25/ PY - 2006/10/27/pubmed PY - 2009/10/29/medline PY - 2006/10/27/entrez SP - 241 EP - 9 JF - Biology of reproduction JO - Biol Reprod VL - 76 IS - 2 N2 - Arginine and ornithine are known to be important for various biological processes in the testis, but the delivery of extracellular cationic amino acids to the seminiferous tubule cells remains poorly understood. We investigated the activity and expression of cationic amino acid transporters in isolated rat Sertoli cells, peritubular cells, pachytene spermatocytes, and early spermatids. We assessed the l-arginine uptake kinetics, Na(+) dependence of transport, profiles of cis inhibition of uptake by cationic and neutral amino acids, and sensitivity to trans stimulation of cationic amino acid transporters, and studied the expression of the genes encoding them by RT-PCR. Our data suggest that l-arginine is taken up by Sertoli cells and peritubular cells, principally via system y(+)L (SLC3A2/SLC7A6) and system y(+) (SLC7A1 and SLC7A2), with system B(0+) making a minor contribution. By contrast, system B(0+), associated with system y(+)L (SLC3A2/SLC7A7 and SLC7A6), made a major contribution to the transport of cationic amino acids in pachytene spermatocytes and early spermatids. Sertoli cells had higher rates of l-arginine transport than the other seminiferous tubule cells. This high efficiency of arginine transport in Sertoli cells and the properties of the y(+)L system predominating in these cells strongly suggest that Sertoli cells play a key role in supplying germ cells with l-arginine and other cationic amino acids. Furthermore, whereas cytokines induce nitric oxide (NO) production in peritubular and Sertoli cells, little or no upregulation of arginine transport by cytokines was observed in these cells. Thus, NO synthesis does not depend on the stimulation of arginine transport in these somatic tubular cells. SN - 1529-7268 UR - https://www.unboundmedicine.com/medline/citation/17065601/Multiple_pathways_for_cationic_amino_acid_transport_in_rat_seminiferous_tubule_cells_ DB - PRIME DP - Unbound Medicine ER -