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Effects of chronic exposure to cadmium on prostate lipids and morphology.
Biometals. 2007 Oct; 20(5):727-41.B

Abstract

Cadmium is an environmental toxic metal implicated in human prostate carcinogenesis. The mechanism of its toxicity is not fully understood. Previously, we showed that cadmium exposure induces oxidative stress, especially lipid peroxidation. This study evaluates the effect of chronic exposure to 0.886 mM of cadmium (Cd) per liter in the drinking water on prostate lipid content and metabolism in Wistar rats. We determined the lipid profile and measured the expression of lipogenic enzymes: FAS, GPAT, LPL, DGAT-1, DGAT-2, ACO, CPT-1 and CT, and of certain factors involved in lipid regulation and fatty acid transporters: FAT/CD36, E-FABP, SREBP-2, PPAR-gamma and PPAR-alpha by RT-PCR. Ultrastructure was analyzed by electron microscopy and, as prostate is an androgen controlled gland, AR expression was measured by RT-PCR and Western blot. Cd altered the prostatic lipid profile. Triglycerides (TG) and esterified cholesterol (EC) decreased, free cholesterol (FC) and phospholipids (PL) increased and total cholesterol (TC) did not change. FAS, MDH and IDH activities did not vary but G6PDH decreased significantly in Cd group. Regarding TG synthesis, DGAT-1 decreased while GPAT increased and FAS, LPL and DGAT-2 remained unchanged. Regarding beta oxidation, CPT-1 increased while ACO expression decreased in Cd group. In the PL pathway, CT expression was increased. All these results would justify the decrease of TG in Cd group when compared to control. In the cholesterol metabolic pathway, HMGCoAR and SREBP-2 increased. PPAR-alpha increased but PPAR-gamma did not change. Regarding fatty acid transporters, FAT/CD36 decreased, while E-FABP increased. AR mRNA and protein expression decreased. Ultrastructural analysis showed a decrease in lipid droplets and signs of cellular damage in the Cd group. Cadmium exposure induces important changes in prostatic lipid profile and metabolism, confirmed by the morphology analyses, which also showed signs of cellular damage. These results could be important to further understanding the complex mechanism of cadmium toxicity in prostate and in the development of better treatments for people and animals exposed to the heavy metal.

Authors+Show Affiliations

Laboratorio de Bioquímica Molecular, Departamento de Bioquímica y Ciencias Biológicas, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Avenida Ejército de los Andes 950, 5700, San Luis, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17066326

Citation

Alvarez, Silvina Mónica, et al. "Effects of Chronic Exposure to Cadmium On Prostate Lipids and Morphology." Biometals : an International Journal On the Role of Metal Ions in Biology, Biochemistry, and Medicine, vol. 20, no. 5, 2007, pp. 727-41.
Alvarez SM, Gómez NN, Scardapane L, et al. Effects of chronic exposure to cadmium on prostate lipids and morphology. Biometals. 2007;20(5):727-41.
Alvarez, S. M., Gómez, N. N., Scardapane, L., Fornés, M. W., & Giménez, M. S. (2007). Effects of chronic exposure to cadmium on prostate lipids and morphology. Biometals : an International Journal On the Role of Metal Ions in Biology, Biochemistry, and Medicine, 20(5), 727-41.
Alvarez SM, et al. Effects of Chronic Exposure to Cadmium On Prostate Lipids and Morphology. Biometals. 2007;20(5):727-41. PubMed PMID: 17066326.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of chronic exposure to cadmium on prostate lipids and morphology. AU - Alvarez,Silvina Mónica, AU - Gómez,Nidia Noemí, AU - Scardapane,Luis, AU - Fornés,Miguel Walter, AU - Giménez,María Sofía, Y1 - 2006/10/26/ PY - 2006/06/26/received PY - 2006/08/23/accepted PY - 2006/10/27/pubmed PY - 2007/12/21/medline PY - 2006/10/27/entrez SP - 727 EP - 41 JF - Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine JO - Biometals VL - 20 IS - 5 N2 - Cadmium is an environmental toxic metal implicated in human prostate carcinogenesis. The mechanism of its toxicity is not fully understood. Previously, we showed that cadmium exposure induces oxidative stress, especially lipid peroxidation. This study evaluates the effect of chronic exposure to 0.886 mM of cadmium (Cd) per liter in the drinking water on prostate lipid content and metabolism in Wistar rats. We determined the lipid profile and measured the expression of lipogenic enzymes: FAS, GPAT, LPL, DGAT-1, DGAT-2, ACO, CPT-1 and CT, and of certain factors involved in lipid regulation and fatty acid transporters: FAT/CD36, E-FABP, SREBP-2, PPAR-gamma and PPAR-alpha by RT-PCR. Ultrastructure was analyzed by electron microscopy and, as prostate is an androgen controlled gland, AR expression was measured by RT-PCR and Western blot. Cd altered the prostatic lipid profile. Triglycerides (TG) and esterified cholesterol (EC) decreased, free cholesterol (FC) and phospholipids (PL) increased and total cholesterol (TC) did not change. FAS, MDH and IDH activities did not vary but G6PDH decreased significantly in Cd group. Regarding TG synthesis, DGAT-1 decreased while GPAT increased and FAS, LPL and DGAT-2 remained unchanged. Regarding beta oxidation, CPT-1 increased while ACO expression decreased in Cd group. In the PL pathway, CT expression was increased. All these results would justify the decrease of TG in Cd group when compared to control. In the cholesterol metabolic pathway, HMGCoAR and SREBP-2 increased. PPAR-alpha increased but PPAR-gamma did not change. Regarding fatty acid transporters, FAT/CD36 decreased, while E-FABP increased. AR mRNA and protein expression decreased. Ultrastructural analysis showed a decrease in lipid droplets and signs of cellular damage in the Cd group. Cadmium exposure induces important changes in prostatic lipid profile and metabolism, confirmed by the morphology analyses, which also showed signs of cellular damage. These results could be important to further understanding the complex mechanism of cadmium toxicity in prostate and in the development of better treatments for people and animals exposed to the heavy metal. SN - 0966-0844 UR - https://www.unboundmedicine.com/medline/citation/17066326/Effects_of_chronic_exposure_to_cadmium_on_prostate_lipids_and_morphology_ L2 - https://doi.org/10.1007/s10534-006-9036-9 DB - PRIME DP - Unbound Medicine ER -