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Constitutive and IFNgamma-induced activation of MHC2TA promoter type III in human melanoma cell lines is governed by separate regulatory elements within the PIII upstream regulatory region.
Mol Immunol. 2007 Mar; 44(8):2036-46.MI

Abstract

Cell lines established from tumor tissue of cutaneous melanoma biopsies often display constitutive and IFNgamma-inducible expression of MHC class II molecules. The expression of MHC class II molecules in melanoma is associated with an overall poor prognosis and unfavorable clinical outcome. We have analyzed the DNA elements and interacting transcription factors that control the constitutive and IFNgamma-inducible expression of the class II transactivator (CIITA), a co-activator essential for transcription of all MHC class II genes. Our studies reveal the activation of multiple CIITA promoter regions (CIITA-PII, -PIII and -PIV) in melanoma cell lines for both the constitutive and IFNgamma-inducible expression of MHC class II molecules. Furthermore, we show that constitutive and IFNgamma-inducible expression of the CIITA-PIII isoform is governed by separate regulatory elements within the PIII upstream regulatory region (PURR). Similarly constitutive activation in melanoma of CIITA-PII, CIITA-PIII, and CIITA-PIV does not require components of the IFNgamma signaling pathway. However, these components are readily recruited to the PURR and CIITA-PIV after exposure of cells to IFNgamma and account for the IFNgamma-induced expression of CIITA. Together, our data reveal the contribution of distinct elements and factors in the constitutive and IFNgamma-inducible expression of CIITA in melanoma cell lines of the skin.

Authors+Show Affiliations

Division of Molecular Biology, Department of Immunohematology and Blood Transfusion, Building 1, E3-Q, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17067677

Citation

van der Stoep, Nienke, et al. "Constitutive and IFNgamma-induced Activation of MHC2TA Promoter Type III in Human Melanoma Cell Lines Is Governed By Separate Regulatory Elements Within the PIII Upstream Regulatory Region." Molecular Immunology, vol. 44, no. 8, 2007, pp. 2036-46.
van der Stoep N, Quinten E, Alblas G, et al. Constitutive and IFNgamma-induced activation of MHC2TA promoter type III in human melanoma cell lines is governed by separate regulatory elements within the PIII upstream regulatory region. Mol Immunol. 2007;44(8):2036-46.
van der Stoep, N., Quinten, E., Alblas, G., Plancke, A., van Eggermond, M. C., Holling, T. M., & van den Elsen, P. J. (2007). Constitutive and IFNgamma-induced activation of MHC2TA promoter type III in human melanoma cell lines is governed by separate regulatory elements within the PIII upstream regulatory region. Molecular Immunology, 44(8), 2036-46.
van der Stoep N, et al. Constitutive and IFNgamma-induced Activation of MHC2TA Promoter Type III in Human Melanoma Cell Lines Is Governed By Separate Regulatory Elements Within the PIII Upstream Regulatory Region. Mol Immunol. 2007;44(8):2036-46. PubMed PMID: 17067677.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Constitutive and IFNgamma-induced activation of MHC2TA promoter type III in human melanoma cell lines is governed by separate regulatory elements within the PIII upstream regulatory region. AU - van der Stoep,Nienke, AU - Quinten,Edwin, AU - Alblas,Gabrielle, AU - Plancke,Aurelie, AU - van Eggermond,Marja C J A, AU - Holling,Tjadine M, AU - van den Elsen,Peter J, Y1 - 2006/10/25/ PY - 2006/08/23/received PY - 2006/09/11/revised PY - 2006/09/12/accepted PY - 2006/10/28/pubmed PY - 2007/3/30/medline PY - 2006/10/28/entrez SP - 2036 EP - 46 JF - Molecular immunology JO - Mol Immunol VL - 44 IS - 8 N2 - Cell lines established from tumor tissue of cutaneous melanoma biopsies often display constitutive and IFNgamma-inducible expression of MHC class II molecules. The expression of MHC class II molecules in melanoma is associated with an overall poor prognosis and unfavorable clinical outcome. We have analyzed the DNA elements and interacting transcription factors that control the constitutive and IFNgamma-inducible expression of the class II transactivator (CIITA), a co-activator essential for transcription of all MHC class II genes. Our studies reveal the activation of multiple CIITA promoter regions (CIITA-PII, -PIII and -PIV) in melanoma cell lines for both the constitutive and IFNgamma-inducible expression of MHC class II molecules. Furthermore, we show that constitutive and IFNgamma-inducible expression of the CIITA-PIII isoform is governed by separate regulatory elements within the PIII upstream regulatory region (PURR). Similarly constitutive activation in melanoma of CIITA-PII, CIITA-PIII, and CIITA-PIV does not require components of the IFNgamma signaling pathway. However, these components are readily recruited to the PURR and CIITA-PIV after exposure of cells to IFNgamma and account for the IFNgamma-induced expression of CIITA. Together, our data reveal the contribution of distinct elements and factors in the constitutive and IFNgamma-inducible expression of CIITA in melanoma cell lines of the skin. SN - 0161-5890 UR - https://www.unboundmedicine.com/medline/citation/17067677/Constitutive_and_IFNgamma_induced_activation_of_MHC2TA_promoter_type_III_in_human_melanoma_cell_lines_is_governed_by_separate_regulatory_elements_within_the_PIII_upstream_regulatory_region_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0161-5890(06)00594-3 DB - PRIME DP - Unbound Medicine ER -