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Insulin-like growth factor-II (IGF-II), IGF-binding protein-3 (IGFBP-3), and IGFBP-4 in follicular fluid are associated with oocyte maturation and embryo development.
Fertil Steril 2006; 86(5):1392-401FS

Abstract

OBJECTIVE

The objective of this study was to investigate the association between follicular fluid (FF) levels of insulin-like growth factors (IGFs), IGF binding proteins (IGFBPs), and pregnancy-associated plasma protein-A (PAPP-A), which is a protease for IGFBP-4, and the quality of subsequent embryo development from in vitro fertilized oocytes aspirated from the same follicle.

DESIGN

Prospective study.

SETTING

University infertility clinic and academic research laboratory.

PATIENT(S)

One hundred sixty-two infertile women undergoing controlled ovarian hyperstimulation for IVF and embryo transfer were recruited in a university hospital.

INTERVENTION(S)

During oocyte retrieval, samples of 225 FFs and matched mature oocytes were collected and studied.

MAIN OUTCOME MEASURE(S)

Concentrations of FF IGF-I, IGF-II, IGFBP-1, IGFBP-3, IGFBP-4, and PAPP-A were determined using ELISA. Progesterone secretion by cultured granulosa cells (GC) was measured by RIA.

RESULT(S)

Levels of IGF-II, IGFBP-3, and IGFBP-4 in FF on the day of oocyte retrieval were significantly correlated with embryo scores on day 3 (72 hours after oocyte retrieval). Levels of IGF-II, IGFBP-3, and IGFBP-4 in FF from follicles in which oocytes developed into day 2 embryos (48 hours after oocyte retrieval; 20 >or= embryo score >or= 6) after fertilization were significantly higher than those from follicles in which oocytes were unable to be fertilized and were arrested in embryo development on day 2, whereas the levels of PAPP-A were significantly lower in the former than the latter group. Using multiple regression analysis, we found that high levels of IGFBP-3 and IGFBP-4 combined with low levels of PAPP-A in FF were significantly correlated with successful fertilization and early development into day 2 embryos. In contrast, high FF IGFBP-1 and IGFBP-4 in combination with low FF IGF-I were significantly correlated with a later (day 2-day 3) embryo development. A significant stimulation of P secretion in cultured GCs by the combination of recombinant IGF-II, IGFBP-3, and IGFBP-4 further strengthened these proteins' functional roles in promoting late follicular development.

CONCLUSION(S)

High IGF-II, IGFBP-3, IGFBP-4, and low PAPP-A levels in FF at the time of oocyte retrieval suggest better oocyte maturation and early embryo development (within 48 hours after oocyte retrieval), whereas high IGFBP-1, IGFBP-4, and low FF IGF-I levels may favor later embryo development (between 48 and 72 hours after oocyte retrieval).

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Lin-Kou Medical Center, Taoyuan, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17070193

Citation

Wang, Tzu-Hao, et al. "Insulin-like Growth factor-II (IGF-II), IGF-binding Protein-3 (IGFBP-3), and IGFBP-4 in Follicular Fluid Are Associated With Oocyte Maturation and Embryo Development." Fertility and Sterility, vol. 86, no. 5, 2006, pp. 1392-401.
Wang TH, Chang CL, Wu HM, et al. Insulin-like growth factor-II (IGF-II), IGF-binding protein-3 (IGFBP-3), and IGFBP-4 in follicular fluid are associated with oocyte maturation and embryo development. Fertil Steril. 2006;86(5):1392-401.
Wang, T. H., Chang, C. L., Wu, H. M., Chiu, Y. M., Chen, C. K., & Wang, H. S. (2006). Insulin-like growth factor-II (IGF-II), IGF-binding protein-3 (IGFBP-3), and IGFBP-4 in follicular fluid are associated with oocyte maturation and embryo development. Fertility and Sterility, 86(5), pp. 1392-401.
Wang TH, et al. Insulin-like Growth factor-II (IGF-II), IGF-binding Protein-3 (IGFBP-3), and IGFBP-4 in Follicular Fluid Are Associated With Oocyte Maturation and Embryo Development. Fertil Steril. 2006;86(5):1392-401. PubMed PMID: 17070193.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Insulin-like growth factor-II (IGF-II), IGF-binding protein-3 (IGFBP-3), and IGFBP-4 in follicular fluid are associated with oocyte maturation and embryo development. AU - Wang,Tzu-Hao, AU - Chang,Chia-Lin, AU - Wu,Hsien-Ming, AU - Chiu,Ya-Ming, AU - Chen,Chun-Kai, AU - Wang,Hsin-Shih, PY - 2005/11/25/received PY - 2006/03/29/revised PY - 2006/03/29/accepted PY - 2006/10/31/pubmed PY - 2006/12/9/medline PY - 2006/10/31/entrez SP - 1392 EP - 401 JF - Fertility and sterility JO - Fertil. Steril. VL - 86 IS - 5 N2 - OBJECTIVE: The objective of this study was to investigate the association between follicular fluid (FF) levels of insulin-like growth factors (IGFs), IGF binding proteins (IGFBPs), and pregnancy-associated plasma protein-A (PAPP-A), which is a protease for IGFBP-4, and the quality of subsequent embryo development from in vitro fertilized oocytes aspirated from the same follicle. DESIGN: Prospective study. SETTING: University infertility clinic and academic research laboratory. PATIENT(S): One hundred sixty-two infertile women undergoing controlled ovarian hyperstimulation for IVF and embryo transfer were recruited in a university hospital. INTERVENTION(S): During oocyte retrieval, samples of 225 FFs and matched mature oocytes were collected and studied. MAIN OUTCOME MEASURE(S): Concentrations of FF IGF-I, IGF-II, IGFBP-1, IGFBP-3, IGFBP-4, and PAPP-A were determined using ELISA. Progesterone secretion by cultured granulosa cells (GC) was measured by RIA. RESULT(S): Levels of IGF-II, IGFBP-3, and IGFBP-4 in FF on the day of oocyte retrieval were significantly correlated with embryo scores on day 3 (72 hours after oocyte retrieval). Levels of IGF-II, IGFBP-3, and IGFBP-4 in FF from follicles in which oocytes developed into day 2 embryos (48 hours after oocyte retrieval; 20 >or= embryo score >or= 6) after fertilization were significantly higher than those from follicles in which oocytes were unable to be fertilized and were arrested in embryo development on day 2, whereas the levels of PAPP-A were significantly lower in the former than the latter group. Using multiple regression analysis, we found that high levels of IGFBP-3 and IGFBP-4 combined with low levels of PAPP-A in FF were significantly correlated with successful fertilization and early development into day 2 embryos. In contrast, high FF IGFBP-1 and IGFBP-4 in combination with low FF IGF-I were significantly correlated with a later (day 2-day 3) embryo development. A significant stimulation of P secretion in cultured GCs by the combination of recombinant IGF-II, IGFBP-3, and IGFBP-4 further strengthened these proteins' functional roles in promoting late follicular development. CONCLUSION(S): High IGF-II, IGFBP-3, IGFBP-4, and low PAPP-A levels in FF at the time of oocyte retrieval suggest better oocyte maturation and early embryo development (within 48 hours after oocyte retrieval), whereas high IGFBP-1, IGFBP-4, and low FF IGF-I levels may favor later embryo development (between 48 and 72 hours after oocyte retrieval). SN - 1556-5653 UR - https://www.unboundmedicine.com/medline/citation/17070193/Insulin_like_growth_factor_II__IGF_II__IGF_binding_protein_3__IGFBP_3__and_IGFBP_4_in_follicular_fluid_are_associated_with_oocyte_maturation_and_embryo_development_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0015-0282(06)01516-0 DB - PRIME DP - Unbound Medicine ER -