TY - JOUR T1 - Optimization of medium and process parameters for the production of inulinase from a newly isolated Kluyveromyces marxianus YS-1. AU - Singh,R S, AU - Sooch,Balwinder Singh, AU - Puri,Munish, Y1 - 2006/10/27/ PY - 2006/07/06/received PY - 2006/08/31/revised PY - 2006/09/07/accepted PY - 2006/10/31/pubmed PY - 2007/8/1/medline PY - 2006/10/31/entrez SP - 2518 EP - 25 JF - Bioresource technology JO - Bioresour Technol VL - 98 IS - 13 N2 - A newly isolated strain of Kluyveromyces marxianus YS-1 was used for the production of extra cellular inulinase in a medium containing inulin, meat extract, CaCl2 and sodium dodecyl sulphate (SDS). Fermentation medium pH 6.5, cultivation temperature 30 degrees C and 5% (v/v) inoculum of 12 h-old culture were optimal for enzyme production (30.8 IU/ml) with a fermentation time of 72 h at shake flask level. Raw inulin (2%, w/v) extracted from dahlia tubers by processing at 15 kg/cm2 for 10 min was optimum for bioreactor studies. Maximum enzyme production (55.4 IU/ml) was obtained at an agitation rate of 200 rpm and aeration of 0.75 vvm in a stirred tank reactor with a fermentation time of 60 h. SN - 0960-8524 UR - https://www.unboundmedicine.com/medline/citation/17071081/Optimization_of_medium_and_process_parameters_for_the_production_of_inulinase_from_a_newly_isolated_Kluyveromyces_marxianus_YS_1_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0960-8524(06)00466-4 DB - PRIME DP - Unbound Medicine ER -