Tags

Type your tag names separated by a space and hit enter

Fluidization of membrane lipids enhances the tolerance of Saccharomyces cerevisiae to freezing and salt stress.
Appl Environ Microbiol. 2007 Jan; 73(1):110-6.AE

Abstract

Unsaturated fatty acids play an essential role in the biophysical characteristics of cell membranes and determine the proper function of membrane-attached proteins. Thus, the ability of cells to alter the degree of unsaturation in their membranes is an important factor in cellular acclimatization to environmental conditions. Many eukaryotic organisms can synthesize dienoic fatty acids, but Saccharomyces cerevisiae can introduce only a single double bond at the Delta(9) position. We expressed two sunflower (Helianthus annuus) oleate Delta(12) desaturases encoded by FAD2-1 and FAD2-3 in yeast cells of the wild-type W303-1A strain (trp1) and analyzed their effects on growth and stress tolerance. Production of the heterologous desaturases increased the content of dienoic fatty acids, especially 18:2Delta(9,12), the unsaturation index, and the fluidity of the yeast membrane. The total fatty acid content remained constant, and the level of monounsaturated fatty acids decreased. Growth at 15 degrees C was reduced in the FAD2 strains, probably due to tryptophan auxotrophy, since the trp1 (TRP1) transformants that produced the sunflower desaturases grew as well as the control strain did. Our results suggest that changes in the fluidity of the lipid bilayer affect tryptophan uptake and/or the correct targeting of tryptophan transporters. The expression of the sunflower desaturases, in either Trp(+) or Trp(-) strains, increased NaCl tolerance. Production of dienoic fatty acids increased the tolerance to freezing of wild-type cells preincubated at 30 degrees C or 15 degrees C. Thus, membrane fluidity is an essential determinant of stress resistance in S. cerevisiae, and engineering of membrane lipids has the potential to be a useful tool of increasing the tolerance to freezing in industrial strains.

Authors+Show Affiliations

Department of Biotechnology, Instituto de Agroquímica y Tecnología de los Alimentos, Consejo Superior de Investigaciones Científicas, E-46100 Burjassot, Valencia, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17071783

Citation

Rodríguez-Vargas, Sonia, et al. "Fluidization of Membrane Lipids Enhances the Tolerance of Saccharomyces Cerevisiae to Freezing and Salt Stress." Applied and Environmental Microbiology, vol. 73, no. 1, 2007, pp. 110-6.
Rodríguez-Vargas S, Sánchez-García A, Martínez-Rivas JM, et al. Fluidization of membrane lipids enhances the tolerance of Saccharomyces cerevisiae to freezing and salt stress. Appl Environ Microbiol. 2007;73(1):110-6.
Rodríguez-Vargas, S., Sánchez-García, A., Martínez-Rivas, J. M., Prieto, J. A., & Randez-Gil, F. (2007). Fluidization of membrane lipids enhances the tolerance of Saccharomyces cerevisiae to freezing and salt stress. Applied and Environmental Microbiology, 73(1), 110-6.
Rodríguez-Vargas S, et al. Fluidization of Membrane Lipids Enhances the Tolerance of Saccharomyces Cerevisiae to Freezing and Salt Stress. Appl Environ Microbiol. 2007;73(1):110-6. PubMed PMID: 17071783.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fluidization of membrane lipids enhances the tolerance of Saccharomyces cerevisiae to freezing and salt stress. AU - Rodríguez-Vargas,Sonia, AU - Sánchez-García,Alicia, AU - Martínez-Rivas,Jose Manuel, AU - Prieto,Jose Antonio, AU - Randez-Gil,Francisca, Y1 - 2006/10/27/ PY - 2006/10/31/pubmed PY - 2007/5/26/medline PY - 2006/10/31/entrez SP - 110 EP - 6 JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 73 IS - 1 N2 - Unsaturated fatty acids play an essential role in the biophysical characteristics of cell membranes and determine the proper function of membrane-attached proteins. Thus, the ability of cells to alter the degree of unsaturation in their membranes is an important factor in cellular acclimatization to environmental conditions. Many eukaryotic organisms can synthesize dienoic fatty acids, but Saccharomyces cerevisiae can introduce only a single double bond at the Delta(9) position. We expressed two sunflower (Helianthus annuus) oleate Delta(12) desaturases encoded by FAD2-1 and FAD2-3 in yeast cells of the wild-type W303-1A strain (trp1) and analyzed their effects on growth and stress tolerance. Production of the heterologous desaturases increased the content of dienoic fatty acids, especially 18:2Delta(9,12), the unsaturation index, and the fluidity of the yeast membrane. The total fatty acid content remained constant, and the level of monounsaturated fatty acids decreased. Growth at 15 degrees C was reduced in the FAD2 strains, probably due to tryptophan auxotrophy, since the trp1 (TRP1) transformants that produced the sunflower desaturases grew as well as the control strain did. Our results suggest that changes in the fluidity of the lipid bilayer affect tryptophan uptake and/or the correct targeting of tryptophan transporters. The expression of the sunflower desaturases, in either Trp(+) or Trp(-) strains, increased NaCl tolerance. Production of dienoic fatty acids increased the tolerance to freezing of wild-type cells preincubated at 30 degrees C or 15 degrees C. Thus, membrane fluidity is an essential determinant of stress resistance in S. cerevisiae, and engineering of membrane lipids has the potential to be a useful tool of increasing the tolerance to freezing in industrial strains. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/17071783/Fluidization_of_membrane_lipids_enhances_the_tolerance_of_Saccharomyces_cerevisiae_to_freezing_and_salt_stress_ L2 - http://aem.asm.org/cgi/pmidlookup?view=long&pmid=17071783 DB - PRIME DP - Unbound Medicine ER -