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Occurrence, prevalence and genetic environment of CTX-M beta-lactamases in Enterobacteriaceae from Indian hospitals.
J Antimicrob Chemother. 2006 Dec; 58(6):1260-3.JA

Abstract

OBJECTIVES

To determine occurrence, prevalence and CTX-M genotypes produced by Enterobacteriaceae from clinical samples from three geographically distant Indian hospitals and to detect linkage of IS26 with bla(CTX-M) and map its precise insertion position.

METHODS

A total of 130, non-duplicate Escherichia coli and Klebsiella pneumoniae resistant to a third-generation cephalosporin (3GC) from three Indian centres were screened for extended-spectrum beta-lactamase (ESBL) production using phenotypic detection methods. All isolates were screened for bla(CTX-M) using multiplex PCR. Precise CTX-M genotype was identified using reverse-line hybridization. All CTX-M-producing isolates were screened for linkage of IS26 with bla(CTX-M). DNA sequencing was used to map the exact insertion position of this mobile element.

RESULTS

Ninety-five of 130 3GC-resistant (73%) (73% of total E. coli, 72% of total K. pneumoniae) isolates were found to carry bla(CTX-M-15). No other CTX-M genotype was detected. IS26 linkage with bla(CTX-M-15) was detected in 31% of isolates carrying bla(CTX-M-15). DNA sequencing revealed variable insertion of this mobile element within tnpA of ISEcp1. RAPD-PCR typing demonstrated great diversity in isolates carrying bla(CTX-M-15); no predominant clone was identified.

CONCLUSIONS

In contrast with other studies where greater diversity exists, CTX-M-15 was the only CTX-M ESBL produced in this Indian collection of unrelated E. coli and K. pneumoniae. This is the first systematic survey report from India detecting CTX-M-type beta-lactamases This is also the first report indicating such high mobility/diversity of insertion of IS26 in close association with bla(CTX-M) in a single bacterial collection.

Authors+Show Affiliations

Antimicrobial Agents Research Group, Institute of Biomedical Research, University of Birmingham, Vincent Drive, Edgbaston, Birmingham B15 2TT, UK.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17071956

Citation

Ensor, V M., et al. "Occurrence, Prevalence and Genetic Environment of CTX-M Beta-lactamases in Enterobacteriaceae From Indian Hospitals." The Journal of Antimicrobial Chemotherapy, vol. 58, no. 6, 2006, pp. 1260-3.
Ensor VM, Shahid M, Evans JT, et al. Occurrence, prevalence and genetic environment of CTX-M beta-lactamases in Enterobacteriaceae from Indian hospitals. J Antimicrob Chemother. 2006;58(6):1260-3.
Ensor, V. M., Shahid, M., Evans, J. T., & Hawkey, P. M. (2006). Occurrence, prevalence and genetic environment of CTX-M beta-lactamases in Enterobacteriaceae from Indian hospitals. The Journal of Antimicrobial Chemotherapy, 58(6), 1260-3.
Ensor VM, et al. Occurrence, Prevalence and Genetic Environment of CTX-M Beta-lactamases in Enterobacteriaceae From Indian Hospitals. J Antimicrob Chemother. 2006;58(6):1260-3. PubMed PMID: 17071956.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Occurrence, prevalence and genetic environment of CTX-M beta-lactamases in Enterobacteriaceae from Indian hospitals. AU - Ensor,V M, AU - Shahid,M, AU - Evans,J T, AU - Hawkey,P M, Y1 - 2006/10/28/ PY - 2006/10/31/pubmed PY - 2007/3/23/medline PY - 2006/10/31/entrez SP - 1260 EP - 3 JF - The Journal of antimicrobial chemotherapy JO - J Antimicrob Chemother VL - 58 IS - 6 N2 - OBJECTIVES: To determine occurrence, prevalence and CTX-M genotypes produced by Enterobacteriaceae from clinical samples from three geographically distant Indian hospitals and to detect linkage of IS26 with bla(CTX-M) and map its precise insertion position. METHODS: A total of 130, non-duplicate Escherichia coli and Klebsiella pneumoniae resistant to a third-generation cephalosporin (3GC) from three Indian centres were screened for extended-spectrum beta-lactamase (ESBL) production using phenotypic detection methods. All isolates were screened for bla(CTX-M) using multiplex PCR. Precise CTX-M genotype was identified using reverse-line hybridization. All CTX-M-producing isolates were screened for linkage of IS26 with bla(CTX-M). DNA sequencing was used to map the exact insertion position of this mobile element. RESULTS: Ninety-five of 130 3GC-resistant (73%) (73% of total E. coli, 72% of total K. pneumoniae) isolates were found to carry bla(CTX-M-15). No other CTX-M genotype was detected. IS26 linkage with bla(CTX-M-15) was detected in 31% of isolates carrying bla(CTX-M-15). DNA sequencing revealed variable insertion of this mobile element within tnpA of ISEcp1. RAPD-PCR typing demonstrated great diversity in isolates carrying bla(CTX-M-15); no predominant clone was identified. CONCLUSIONS: In contrast with other studies where greater diversity exists, CTX-M-15 was the only CTX-M ESBL produced in this Indian collection of unrelated E. coli and K. pneumoniae. This is the first systematic survey report from India detecting CTX-M-type beta-lactamases This is also the first report indicating such high mobility/diversity of insertion of IS26 in close association with bla(CTX-M) in a single bacterial collection. SN - 0305-7453 UR - https://www.unboundmedicine.com/medline/citation/17071956/Occurrence_prevalence_and_genetic_environment_of_CTX_M_beta_lactamases_in_Enterobacteriaceae_from_Indian_hospitals_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dkl422 DB - PRIME DP - Unbound Medicine ER -