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Mutation in an adaptor protein PDZK1 affects transport activity of organic cation transporter OCTNs and oligopeptide transporter PEPT2.
Drug Metab Pharmacokinet. 2006 Oct; 21(5):375-83.DM

Abstract

Genetic polymorphisms in xenobiotic transporters have recently been clarified to be associated with change in drug distribution and disposition. To expand on recent identification of direct interaction and functional regulation of several transporters by a PDZ (PSD95, Dlg and ZO1) domain containing protein PDZK1, the effect of mutation in PDZK1 on transport activity and subcellular localization of organic cation/carnitine transporters OCTN1 and OCTN2, and oligopeptide transporter PEPT2 was examined in the present study. HEK293 cells stably expressing a mutant transcript PDZK1-E195K (HEK293/PDZK1-E195K) were constructed, followed by transient transfection of cDNA for each transporter. Uptake of tetraethylammonium by OCTN1 was much higher in HEK293/PDZK1 cells, compared with that in the parent HEK293 cells, the uptake in HEK293/PDZK1-E195K cells showing middle range between the two values. Such difference in transport activity was accounted for the difference in transport capacity, with minimal change in affinity of OCTN1 to the substrate or other compounds. The similar difference among HEK293/PDZK1, HEK293/PDZK1-E195K and HEK293 cells was also observed in transport property of OCTN2 and PEPT2, whereas the difference was not so remarkable in each transporter with the last four amino acids deleted, that has much lower interaction potential with PDZK1. Immunohistochemical analysis indicated that OCTN1 was colocalized with PDZK1 on cell-surface, whereas colocalization with PDZK1-E195K was partially observed in cytoplasmic region. These results suggest a novel hypothesis that mutation in PDZK1 potentially changes transport property of various types of xenobiotic transporters by affecting their subcellular localization, possibly leading to change in disposition of various types of substrate drugs.

Authors+Show Affiliations

Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17072090

Citation

Sugiura, Tomoko, et al. "Mutation in an Adaptor Protein PDZK1 Affects Transport Activity of Organic Cation Transporter OCTNs and Oligopeptide Transporter PEPT2." Drug Metabolism and Pharmacokinetics, vol. 21, no. 5, 2006, pp. 375-83.
Sugiura T, Kato Y, Kubo Y, et al. Mutation in an adaptor protein PDZK1 affects transport activity of organic cation transporter OCTNs and oligopeptide transporter PEPT2. Drug Metab Pharmacokinet. 2006;21(5):375-83.
Sugiura, T., Kato, Y., Kubo, Y., & Tsuji, A. (2006). Mutation in an adaptor protein PDZK1 affects transport activity of organic cation transporter OCTNs and oligopeptide transporter PEPT2. Drug Metabolism and Pharmacokinetics, 21(5), 375-83.
Sugiura T, et al. Mutation in an Adaptor Protein PDZK1 Affects Transport Activity of Organic Cation Transporter OCTNs and Oligopeptide Transporter PEPT2. Drug Metab Pharmacokinet. 2006;21(5):375-83. PubMed PMID: 17072090.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mutation in an adaptor protein PDZK1 affects transport activity of organic cation transporter OCTNs and oligopeptide transporter PEPT2. AU - Sugiura,Tomoko, AU - Kato,Yukio, AU - Kubo,Yoshiyuki, AU - Tsuji,Akira, PY - 2006/10/31/pubmed PY - 2007/1/17/medline PY - 2006/10/31/entrez SP - 375 EP - 83 JF - Drug metabolism and pharmacokinetics JO - Drug Metab Pharmacokinet VL - 21 IS - 5 N2 - Genetic polymorphisms in xenobiotic transporters have recently been clarified to be associated with change in drug distribution and disposition. To expand on recent identification of direct interaction and functional regulation of several transporters by a PDZ (PSD95, Dlg and ZO1) domain containing protein PDZK1, the effect of mutation in PDZK1 on transport activity and subcellular localization of organic cation/carnitine transporters OCTN1 and OCTN2, and oligopeptide transporter PEPT2 was examined in the present study. HEK293 cells stably expressing a mutant transcript PDZK1-E195K (HEK293/PDZK1-E195K) were constructed, followed by transient transfection of cDNA for each transporter. Uptake of tetraethylammonium by OCTN1 was much higher in HEK293/PDZK1 cells, compared with that in the parent HEK293 cells, the uptake in HEK293/PDZK1-E195K cells showing middle range between the two values. Such difference in transport activity was accounted for the difference in transport capacity, with minimal change in affinity of OCTN1 to the substrate or other compounds. The similar difference among HEK293/PDZK1, HEK293/PDZK1-E195K and HEK293 cells was also observed in transport property of OCTN2 and PEPT2, whereas the difference was not so remarkable in each transporter with the last four amino acids deleted, that has much lower interaction potential with PDZK1. Immunohistochemical analysis indicated that OCTN1 was colocalized with PDZK1 on cell-surface, whereas colocalization with PDZK1-E195K was partially observed in cytoplasmic region. These results suggest a novel hypothesis that mutation in PDZK1 potentially changes transport property of various types of xenobiotic transporters by affecting their subcellular localization, possibly leading to change in disposition of various types of substrate drugs. SN - 1347-4367 UR - https://www.unboundmedicine.com/medline/citation/17072090/Mutation_in_an_adaptor_protein_PDZK1_affects_transport_activity_of_organic_cation_transporter_OCTNs_and_oligopeptide_transporter_PEPT2_ L2 - http://joi.jlc.jst.go.jp/JST.JSTAGE/dmpk/21.375?from=PubMed DB - PRIME DP - Unbound Medicine ER -