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Cell wall involvement in the glycerol response to high osmolarity in the halotolerant yeast Debaryomyces hansenii.
Antonie Van Leeuwenhoek. 2007 Apr; 91(3):229-35.AV

Abstract

Osmotic stress was studied through the induction of the gene coding for glycerol 3-phosphate dehydrogenase (DhGPD1) in the halotolerant yeast Debaryomyces hansenii. This yeast responded to modifications in turgor pressure by stimulating the transcription of DhGPD1 when exposed to solutes that cause turgor stress (NaCl or sorbitol), but did not respond to water stress mediated by ethanol. In contrast to what has been documented to occur in Saccharomyces cerevisiae, D. hansenii protoplasts did not show induction in the transcription of DhGPD1 showing a limitation in their response to solute stress. The results presented indicate that the presence of the cell wall is of significance for the induction of DhGPD1 and hence for osmotic regulation in halotolerant D. hansenii. It appears that the main osmosensor that links high osmolarity with glycerol accumulation may be of a different nature in this yeast.

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Authors+Show Affiliations

Thomé PE
Unidad Académica Puerto Morelos, Instituto de Ciencias del Mar y Limnología, UNAM, Avenida Niños Héroes S/N, Puerto Morelos, Quintana Roo, C.P. 77580, México. thome@mar.icmyl.unam.mx

MeSH

AscomycotaCell WallGene Expression Regulation, FungalGlycerolGlycerol-3-Phosphate Dehydrogenase (NAD+)Osmolar ConcentrationRNA, FungalSodium ChlorideSorbitolTranscription, Genetic

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17072537

Citation

Thomé, Patricia E.. "Cell Wall Involvement in the Glycerol Response to High Osmolarity in the Halotolerant Yeast Debaryomyces Hansenii." Antonie Van Leeuwenhoek, vol. 91, no. 3, 2007, pp. 229-35.
Thomé PE. Cell wall involvement in the glycerol response to high osmolarity in the halotolerant yeast Debaryomyces hansenii. Antonie Van Leeuwenhoek. 2007;91(3):229-35.
Thomé, P. E. (2007). Cell wall involvement in the glycerol response to high osmolarity in the halotolerant yeast Debaryomyces hansenii. Antonie Van Leeuwenhoek, 91(3), 229-35.
Thomé PE. Cell Wall Involvement in the Glycerol Response to High Osmolarity in the Halotolerant Yeast Debaryomyces Hansenii. Antonie Van Leeuwenhoek. 2007;91(3):229-35. PubMed PMID: 17072537.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cell wall involvement in the glycerol response to high osmolarity in the halotolerant yeast Debaryomyces hansenii. A1 - Thomé,Patricia E, Y1 - 2006/10/28/ PY - 2005/06/08/received PY - 2006/08/07/accepted PY - 2006/10/31/pubmed PY - 2007/6/21/medline PY - 2006/10/31/entrez SP - 229 EP - 35 JF - Antonie van Leeuwenhoek JO - Antonie Van Leeuwenhoek VL - 91 IS - 3 N2 - Osmotic stress was studied through the induction of the gene coding for glycerol 3-phosphate dehydrogenase (DhGPD1) in the halotolerant yeast Debaryomyces hansenii. This yeast responded to modifications in turgor pressure by stimulating the transcription of DhGPD1 when exposed to solutes that cause turgor stress (NaCl or sorbitol), but did not respond to water stress mediated by ethanol. In contrast to what has been documented to occur in Saccharomyces cerevisiae, D. hansenii protoplasts did not show induction in the transcription of DhGPD1 showing a limitation in their response to solute stress. The results presented indicate that the presence of the cell wall is of significance for the induction of DhGPD1 and hence for osmotic regulation in halotolerant D. hansenii. It appears that the main osmosensor that links high osmolarity with glycerol accumulation may be of a different nature in this yeast. SN - 0003-6072 UR - https://www.unboundmedicine.com/medline/citation/17072537/Cell_wall_involvement_in_the_glycerol_response_to_high_osmolarity_in_the_halotolerant_yeast_Debaryomyces_hansenii_ L2 - https://doi.org/10.1007/s10482-006-9112-8 DB - PRIME DP - Unbound Medicine ER -