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RovM, a novel LysR-type regulator of the virulence activator gene rovA, controls cell invasion, virulence and motility of Yersinia pseudotuberculosis.
Mol Microbiol. 2006 Dec; 62(5):1469-83.MM

Abstract

RovA is a MarR-type transcriptional regulator that controls transcription of rovA, the expression of the primary invasive factor invasin and other virulence genes of Yersinia pseudotuberculosis in response to environmental signals. Using a genetic approach to identify regulatory components that negatively influence rovA expression, we identified a new LysR-type regulatory protein, designated RovM, which exhibits homology to the virulence regulator PecT/HexA of plant pathogenic Erwinia species. DNA-binding studies revealed that RovM interacts specifically with a short binding site between promoters P1 and P2 within the rovA regulatory region and negatively modulates rovA transcription in cooperation with the histone-like protein H-NS. The rovM gene itself is under positive autoregulatory control and is significantly induced during growth in minimal media as shown in regulation studies. Disruption of the rovM gene leads to a significant increase of RovA and invasin synthesis and enhances internalization of Y. pseudotuberculosis into host cells. Finally, we show that a Y. pseudotuberculosis rovM mutant is more virulent than wild type and higher numbers of the bacteria are detectable in gut-associated lymphatic tissues and organs in the mouse infection model system. In contrast, elevated levels of the RovM protein, which exert a positive effect on flagellar motility, severely attenuate the ability of Y. pseudotuberculosis to disseminate to deeper tissues. Together, our data show, that RovM is a key regulator implicated in the environmental control of virulence factors, which are crucial for the initiation of a Yersinia infection.

Authors+Show Affiliations

Institut für Mikrobiologie, Technische Universität Braunschweig, 38106 Braunschweig, Germany.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17074075

Citation

Heroven, Ann Kathrin, and Petra Dersch. "RovM, a Novel LysR-type Regulator of the Virulence Activator Gene rovA, Controls Cell Invasion, Virulence and Motility of Yersinia Pseudotuberculosis." Molecular Microbiology, vol. 62, no. 5, 2006, pp. 1469-83.
Heroven AK, Dersch P. RovM, a novel LysR-type regulator of the virulence activator gene rovA, controls cell invasion, virulence and motility of Yersinia pseudotuberculosis. Mol Microbiol. 2006;62(5):1469-83.
Heroven, A. K., & Dersch, P. (2006). RovM, a novel LysR-type regulator of the virulence activator gene rovA, controls cell invasion, virulence and motility of Yersinia pseudotuberculosis. Molecular Microbiology, 62(5), 1469-83.
Heroven AK, Dersch P. RovM, a Novel LysR-type Regulator of the Virulence Activator Gene rovA, Controls Cell Invasion, Virulence and Motility of Yersinia Pseudotuberculosis. Mol Microbiol. 2006;62(5):1469-83. PubMed PMID: 17074075.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - RovM, a novel LysR-type regulator of the virulence activator gene rovA, controls cell invasion, virulence and motility of Yersinia pseudotuberculosis. AU - Heroven,Ann Kathrin, AU - Dersch,Petra, Y1 - 2006/10/27/ PY - 2006/11/1/pubmed PY - 2007/3/3/medline PY - 2006/11/1/entrez SP - 1469 EP - 83 JF - Molecular microbiology JO - Mol Microbiol VL - 62 IS - 5 N2 - RovA is a MarR-type transcriptional regulator that controls transcription of rovA, the expression of the primary invasive factor invasin and other virulence genes of Yersinia pseudotuberculosis in response to environmental signals. Using a genetic approach to identify regulatory components that negatively influence rovA expression, we identified a new LysR-type regulatory protein, designated RovM, which exhibits homology to the virulence regulator PecT/HexA of plant pathogenic Erwinia species. DNA-binding studies revealed that RovM interacts specifically with a short binding site between promoters P1 and P2 within the rovA regulatory region and negatively modulates rovA transcription in cooperation with the histone-like protein H-NS. The rovM gene itself is under positive autoregulatory control and is significantly induced during growth in minimal media as shown in regulation studies. Disruption of the rovM gene leads to a significant increase of RovA and invasin synthesis and enhances internalization of Y. pseudotuberculosis into host cells. Finally, we show that a Y. pseudotuberculosis rovM mutant is more virulent than wild type and higher numbers of the bacteria are detectable in gut-associated lymphatic tissues and organs in the mouse infection model system. In contrast, elevated levels of the RovM protein, which exert a positive effect on flagellar motility, severely attenuate the ability of Y. pseudotuberculosis to disseminate to deeper tissues. Together, our data show, that RovM is a key regulator implicated in the environmental control of virulence factors, which are crucial for the initiation of a Yersinia infection. SN - 0950-382X UR - https://www.unboundmedicine.com/medline/citation/17074075/RovM_a_novel_LysR_type_regulator_of_the_virulence_activator_gene_rovA_controls_cell_invasion_virulence_and_motility_of_Yersinia_pseudotuberculosis_ L2 - https://doi.org/10.1111/j.1365-2958.2006.05458.x DB - PRIME DP - Unbound Medicine ER -