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Differential regulation of the zebrafish orthopedia 1 gene during fate determination of diencephalic neurons.
BMC Dev Biol. 2006 Oct 30; 6:50.BD

Abstract

BACKGROUND

The homeodomain transcription factor Orthopedia (Otp) is essential in restricting the fate of multiple classes of secreting neurons in the neuroendocrine hypothalamus of vertebrates. However, there is little information on the intercellular factors that regulate Otp expression during development.

RESULTS

Here, we identified two otp orthologues in zebrafish (otp1 and otp2) and explored otp1 in the context of the morphogenetic pathways that specify neuroectodermal regions. During forebrain development, otp1 is expressed in anterior groups of diencephalic cells, positioned in the preoptic area (PO) (anterior alar plate) and the posterior tuberculum (PT) (posterior basal plate). The latter structure is characterized by Tyrosine Hydroxylase (TH)-positive cells, suggesting a role for otp1 in the lineage restriction of catecholaminergic (CA) neurons. Disruptions of Hedgehog (HH) and Fibroblast Growth Factor (FGF) pathways point to the ability of SHH protein to trigger otp1 expression in PO presumptive neuroblasts, with the attenuating effect of Dzip1 and FGF8. In addition, our data disclose otp1 as a determinant of CA neurons in the PT, where otp1 activity is strictly dependent on Nodal signaling and it is not responsive to SHH and FGF.

CONCLUSION

In this study, we pinpoint the evolutionary importance of otp1 transcription factor in cell states of the diencephalon anlage and early neuronal progenitors. Furthermore, our data indicate that morphogenetic mechanisms differentially regulate otp1 expression in alar and basal plates.

Authors+Show Affiliations

Department of Biology, Università degli Studi di Milano, Via Celoria 26, Milano, 20133, Italy. luca.delgiacco@unimi.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17074092

Citation

Del Giacco, Luca, et al. "Differential Regulation of the Zebrafish Orthopedia 1 Gene During Fate Determination of Diencephalic Neurons." BMC Developmental Biology, vol. 6, 2006, p. 50.
Del Giacco L, Sordino P, Pistocchi A, et al. Differential regulation of the zebrafish orthopedia 1 gene during fate determination of diencephalic neurons. BMC Dev Biol. 2006;6:50.
Del Giacco, L., Sordino, P., Pistocchi, A., Andreakis, N., Tarallo, R., Di Benedetto, B., & Cotelli, F. (2006). Differential regulation of the zebrafish orthopedia 1 gene during fate determination of diencephalic neurons. BMC Developmental Biology, 6, 50.
Del Giacco L, et al. Differential Regulation of the Zebrafish Orthopedia 1 Gene During Fate Determination of Diencephalic Neurons. BMC Dev Biol. 2006 Oct 30;6:50. PubMed PMID: 17074092.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential regulation of the zebrafish orthopedia 1 gene during fate determination of diencephalic neurons. AU - Del Giacco,Luca, AU - Sordino,Paolo, AU - Pistocchi,Anna, AU - Andreakis,Nikos, AU - Tarallo,Raffaella, AU - Di Benedetto,Barbara, AU - Cotelli,Franco, Y1 - 2006/10/30/ PY - 2006/08/04/received PY - 2006/10/30/accepted PY - 2006/11/1/pubmed PY - 2007/1/9/medline PY - 2006/11/1/entrez SP - 50 EP - 50 JF - BMC developmental biology JO - BMC Dev. Biol. VL - 6 N2 - BACKGROUND: The homeodomain transcription factor Orthopedia (Otp) is essential in restricting the fate of multiple classes of secreting neurons in the neuroendocrine hypothalamus of vertebrates. However, there is little information on the intercellular factors that regulate Otp expression during development. RESULTS: Here, we identified two otp orthologues in zebrafish (otp1 and otp2) and explored otp1 in the context of the morphogenetic pathways that specify neuroectodermal regions. During forebrain development, otp1 is expressed in anterior groups of diencephalic cells, positioned in the preoptic area (PO) (anterior alar plate) and the posterior tuberculum (PT) (posterior basal plate). The latter structure is characterized by Tyrosine Hydroxylase (TH)-positive cells, suggesting a role for otp1 in the lineage restriction of catecholaminergic (CA) neurons. Disruptions of Hedgehog (HH) and Fibroblast Growth Factor (FGF) pathways point to the ability of SHH protein to trigger otp1 expression in PO presumptive neuroblasts, with the attenuating effect of Dzip1 and FGF8. In addition, our data disclose otp1 as a determinant of CA neurons in the PT, where otp1 activity is strictly dependent on Nodal signaling and it is not responsive to SHH and FGF. CONCLUSION: In this study, we pinpoint the evolutionary importance of otp1 transcription factor in cell states of the diencephalon anlage and early neuronal progenitors. Furthermore, our data indicate that morphogenetic mechanisms differentially regulate otp1 expression in alar and basal plates. SN - 1471-213X UR - https://www.unboundmedicine.com/medline/citation/17074092/Differential_regulation_of_the_zebrafish_orthopedia_1_gene_during_fate_determination_of_diencephalic_neurons_ L2 - https://bmcdevbiol.biomedcentral.com/articles/10.1186/1471-213X-6-50 DB - PRIME DP - Unbound Medicine ER -