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Specific detection of anabasine, nicotine, and nicotine metabolites in urine by liquid chromatography-tandem mass spectrometry.
Am J Clin Pathol. 2006 Dec; 126(6):880-7.AJ

Abstract

The sensitive and specific detection of nicotine, its metabolites, and the tobacco alkaloid, anabasine, is useful in evaluating the success of smoking cessation treatments and detecting tobacco use, passive exposure, and nontobacco nicotine exposure in potential transplant recipients, insurance clients, and elective surgical patients. Rapid sample preparation and extended high-performance liquid chromatographic separation of tobacco alkaloids and metabolites was interfaced with tandem mass spectrometry. By using deuterated internal standards and appropriate confirmatory ion mass transitions, direct injection of centrifugally clarified urine was possible. The method had excellent precision, limit of quantitation, and linearity. The rigorous separation method revealed an interferent of nicotine that had coeluted with anabasine in more rapid chromatography and that may result in tobacco use misclassification. The method provides more specific detection of tobacco exposure and illustrates the potential of centrifugal clarification for sample preparation in the detection of multiple analytes in urine.

Authors+Show Affiliations

Department of Laboratory Medicine, University of Washington Medical Center, Seattle, WA 98195, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17074683

Citation

Hoofnagle, Andrew N., et al. "Specific Detection of Anabasine, Nicotine, and Nicotine Metabolites in Urine By Liquid Chromatography-tandem Mass Spectrometry." American Journal of Clinical Pathology, vol. 126, no. 6, 2006, pp. 880-7.
Hoofnagle AN, Laha TJ, Rainey PM, et al. Specific detection of anabasine, nicotine, and nicotine metabolites in urine by liquid chromatography-tandem mass spectrometry. Am J Clin Pathol. 2006;126(6):880-7.
Hoofnagle, A. N., Laha, T. J., Rainey, P. M., & Sadrzadeh, S. M. (2006). Specific detection of anabasine, nicotine, and nicotine metabolites in urine by liquid chromatography-tandem mass spectrometry. American Journal of Clinical Pathology, 126(6), 880-7.
Hoofnagle AN, et al. Specific Detection of Anabasine, Nicotine, and Nicotine Metabolites in Urine By Liquid Chromatography-tandem Mass Spectrometry. Am J Clin Pathol. 2006;126(6):880-7. PubMed PMID: 17074683.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Specific detection of anabasine, nicotine, and nicotine metabolites in urine by liquid chromatography-tandem mass spectrometry. AU - Hoofnagle,Andrew N, AU - Laha,Thomas J, AU - Rainey,Petrie M, AU - Sadrzadeh,Sayed M H, PY - 2006/11/1/pubmed PY - 2007/1/5/medline PY - 2006/11/1/entrez SP - 880 EP - 7 JF - American journal of clinical pathology JO - Am J Clin Pathol VL - 126 IS - 6 N2 - The sensitive and specific detection of nicotine, its metabolites, and the tobacco alkaloid, anabasine, is useful in evaluating the success of smoking cessation treatments and detecting tobacco use, passive exposure, and nontobacco nicotine exposure in potential transplant recipients, insurance clients, and elective surgical patients. Rapid sample preparation and extended high-performance liquid chromatographic separation of tobacco alkaloids and metabolites was interfaced with tandem mass spectrometry. By using deuterated internal standards and appropriate confirmatory ion mass transitions, direct injection of centrifugally clarified urine was possible. The method had excellent precision, limit of quantitation, and linearity. The rigorous separation method revealed an interferent of nicotine that had coeluted with anabasine in more rapid chromatography and that may result in tobacco use misclassification. The method provides more specific detection of tobacco exposure and illustrates the potential of centrifugal clarification for sample preparation in the detection of multiple analytes in urine. SN - 0002-9173 UR - https://www.unboundmedicine.com/medline/citation/17074683/Specific_detection_of_anabasine_nicotine_and_nicotine_metabolites_in_urine_by_liquid_chromatography_tandem_mass_spectrometry_ L2 - https://academic.oup.com/ajcp/article-lookup/doi/10.1309/LQ8U3UL956ET324X DB - PRIME DP - Unbound Medicine ER -