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Micellar electrokinetic capillary chromatography for fast separation and sensitive determination of melatonin and related indoleamines using end-column amperometric detection.
Electrophoresis. 2006 Nov; 27(21):4230-9.E

Abstract

MEKC was used in conjunction with end-column amperometric detection (AD) at a carbon disc electrode (0.3 mm diameter) for the selective and sensitive determination of melatonin and its five related indoleamines including its precursors and metabolites in the pineal gland. The introduction of a sample stacking technique in injection and the buffer additive SDS in the buffer solution system provided the rapid and sensitive analysis. Optimal buffer conditions (10 mmol/L phosphate containing 20 mmol/L SDS, pH 7.2), detection potential (+1.0 V vs. Ag/AgCl), and electrokinetic injection 10 s with the separation voltage of 24 kV were employed to achieve the baseline separation of six pineal hormones within 15 min. The peak currents and the analyte concentrations have a good linear relationship over the range of 6.0 x 10(-8) 6.0 x 10(-5)mol/L. The detection limits for six pineal hormones by AD are 9.7 to 41.8 nmol/L (equal to 2.0 to 9.7 ng/mL) (S/N = 3), respectively. It is proved to provide about 30- to 250-fold improvement over UV, and be comparable with the sensitive fluorescence detection, which needs pre-column derivatization. The proposed method has been applied for analysis of melatonin and related indoleamines in rat pineal glands. A very simple sample pretreatment procedure, merely involving the homogenization step in perchloric acid, was enough to achieve recoveries in the range of 71 to 127% for all the analytes in the pineal gland.

Authors+Show Affiliations

Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety, Fuzhou University, Department of Chemistry, Fuzhou, P R China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

17075947

Citation

Wu, Xiaoping, et al. "Micellar Electrokinetic Capillary Chromatography for Fast Separation and Sensitive Determination of Melatonin and Related Indoleamines Using End-column Amperometric Detection." Electrophoresis, vol. 27, no. 21, 2006, pp. 4230-9.
Wu X, Wu W, Zhang L, et al. Micellar electrokinetic capillary chromatography for fast separation and sensitive determination of melatonin and related indoleamines using end-column amperometric detection. Electrophoresis. 2006;27(21):4230-9.
Wu, X., Wu, W., Zhang, L., Xie, Z., Qiu, B., & Chen, G. (2006). Micellar electrokinetic capillary chromatography for fast separation and sensitive determination of melatonin and related indoleamines using end-column amperometric detection. Electrophoresis, 27(21), 4230-9.
Wu X, et al. Micellar Electrokinetic Capillary Chromatography for Fast Separation and Sensitive Determination of Melatonin and Related Indoleamines Using End-column Amperometric Detection. Electrophoresis. 2006;27(21):4230-9. PubMed PMID: 17075947.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Micellar electrokinetic capillary chromatography for fast separation and sensitive determination of melatonin and related indoleamines using end-column amperometric detection. AU - Wu,Xiaoping, AU - Wu,Weimin, AU - Zhang,Lan, AU - Xie,Zenghong, AU - Qiu,Bin, AU - Chen,Guonan, PY - 2006/11/1/pubmed PY - 2007/1/31/medline PY - 2006/11/1/entrez SP - 4230 EP - 9 JF - Electrophoresis JO - Electrophoresis VL - 27 IS - 21 N2 - MEKC was used in conjunction with end-column amperometric detection (AD) at a carbon disc electrode (0.3 mm diameter) for the selective and sensitive determination of melatonin and its five related indoleamines including its precursors and metabolites in the pineal gland. The introduction of a sample stacking technique in injection and the buffer additive SDS in the buffer solution system provided the rapid and sensitive analysis. Optimal buffer conditions (10 mmol/L phosphate containing 20 mmol/L SDS, pH 7.2), detection potential (+1.0 V vs. Ag/AgCl), and electrokinetic injection 10 s with the separation voltage of 24 kV were employed to achieve the baseline separation of six pineal hormones within 15 min. The peak currents and the analyte concentrations have a good linear relationship over the range of 6.0 x 10(-8) 6.0 x 10(-5)mol/L. The detection limits for six pineal hormones by AD are 9.7 to 41.8 nmol/L (equal to 2.0 to 9.7 ng/mL) (S/N = 3), respectively. It is proved to provide about 30- to 250-fold improvement over UV, and be comparable with the sensitive fluorescence detection, which needs pre-column derivatization. The proposed method has been applied for analysis of melatonin and related indoleamines in rat pineal glands. A very simple sample pretreatment procedure, merely involving the homogenization step in perchloric acid, was enough to achieve recoveries in the range of 71 to 127% for all the analytes in the pineal gland. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/17075947/Micellar_electrokinetic_capillary_chromatography_for_fast_separation_and_sensitive_determination_of_melatonin_and_related_indoleamines_using_end_column_amperometric_detection_ L2 - https://doi.org/10.1002/elps.200600248 DB - PRIME DP - Unbound Medicine ER -