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Expression of natural cytotoxicity receptors and a2V-ATPase on peripheral blood NK cell subsets in women with recurrent spontaneous abortions and implantation failures.
Am J Reprod Immunol. 2006 Nov-Dec; 56(5-6):312-20.AJ

Abstract

PROBLEM

Natural cytotoxicity receptors (NCRs) are unique markers, which regulate NK cell cytotoxicity and cytokine production. a2V-ATPase is expressed on subsets of PBMC and regulates the extracellular environment, which facilitates NK cytotoxicity or cytokine secretion. In this study, we aim to investigate the expression of NCRs and a2V-ATPase in peripheral blood NK cells of women with recurrent spontaneous abortions (RSA) or implantation failures.

METHOD OF STUDY

Peripheral blood NK cells (CD56(dim) and CD56(bright) were analyzed for the expression of NCRs (NKp46, NKp44 and NKp30) and a2V-ATPase using 3-color flow cytometry in women with RSA (n=24), implantation failures (n=19) or normal healthy women (n=13).

RESULTS

CD56+/NKp46+ cells were markedly decreased (P<0.05) and CD56(bright)/a2V-ATPase+ cells were significantly increased (P<0.05) in women with RSA as compared to those of normal controls. In women with RSA or implantation failures, expression of NKp46, NKp44, NKp30, and a2V-ATPase on CD56(bright) NK cells was significantly up-regulated as compared with those of CD56(dim) NK cells.

CONCLUSION

The differential expression of NCRs and a2V-ATPase in NK cell subsets may suggest dysregulation of NK cytotoxicity and cytokine production in women with RSA and implantation failures.

Authors+Show Affiliations

Department of Microbiology and Immunology, Rosalind Franklin University of Medicine and Science/The Chicago Medical School, North Chicago, IL 60064, USA. a.fukuipon@mac.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17076675

Citation

Fukui, Atsushi, et al. "Expression of Natural Cytotoxicity Receptors and a2V-ATPase On Peripheral Blood NK Cell Subsets in Women With Recurrent Spontaneous Abortions and Implantation Failures." American Journal of Reproductive Immunology (New York, N.Y. : 1989), vol. 56, no. 5-6, 2006, pp. 312-20.
Fukui A, Ntrivalas E, Gilman-Sachs A, et al. Expression of natural cytotoxicity receptors and a2V-ATPase on peripheral blood NK cell subsets in women with recurrent spontaneous abortions and implantation failures. Am J Reprod Immunol. 2006;56(5-6):312-20.
Fukui, A., Ntrivalas, E., Gilman-Sachs, A., Kwak-Kim, J., Lee, S. K., Levine, R., & Beaman, K. (2006). Expression of natural cytotoxicity receptors and a2V-ATPase on peripheral blood NK cell subsets in women with recurrent spontaneous abortions and implantation failures. American Journal of Reproductive Immunology (New York, N.Y. : 1989), 56(5-6), 312-20.
Fukui A, et al. Expression of Natural Cytotoxicity Receptors and a2V-ATPase On Peripheral Blood NK Cell Subsets in Women With Recurrent Spontaneous Abortions and Implantation Failures. Am J Reprod Immunol. 2006;56(5-6):312-20. PubMed PMID: 17076675.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of natural cytotoxicity receptors and a2V-ATPase on peripheral blood NK cell subsets in women with recurrent spontaneous abortions and implantation failures. AU - Fukui,Atsushi, AU - Ntrivalas,Evangelos, AU - Gilman-Sachs,Alice, AU - Kwak-Kim,Joanne, AU - Lee,Sung-Ki, AU - Levine,Rita, AU - Beaman,Kenneth, PY - 2006/11/2/pubmed PY - 2007/3/21/medline PY - 2006/11/2/entrez SP - 312 EP - 20 JF - American journal of reproductive immunology (New York, N.Y. : 1989) JO - Am. J. Reprod. Immunol. VL - 56 IS - 5-6 N2 - PROBLEM: Natural cytotoxicity receptors (NCRs) are unique markers, which regulate NK cell cytotoxicity and cytokine production. a2V-ATPase is expressed on subsets of PBMC and regulates the extracellular environment, which facilitates NK cytotoxicity or cytokine secretion. In this study, we aim to investigate the expression of NCRs and a2V-ATPase in peripheral blood NK cells of women with recurrent spontaneous abortions (RSA) or implantation failures. METHOD OF STUDY: Peripheral blood NK cells (CD56(dim) and CD56(bright) were analyzed for the expression of NCRs (NKp46, NKp44 and NKp30) and a2V-ATPase using 3-color flow cytometry in women with RSA (n=24), implantation failures (n=19) or normal healthy women (n=13). RESULTS: CD56+/NKp46+ cells were markedly decreased (P<0.05) and CD56(bright)/a2V-ATPase+ cells were significantly increased (P<0.05) in women with RSA as compared to those of normal controls. In women with RSA or implantation failures, expression of NKp46, NKp44, NKp30, and a2V-ATPase on CD56(bright) NK cells was significantly up-regulated as compared with those of CD56(dim) NK cells. CONCLUSION: The differential expression of NCRs and a2V-ATPase in NK cell subsets may suggest dysregulation of NK cytotoxicity and cytokine production in women with RSA and implantation failures. SN - 1046-7408 UR - https://www.unboundmedicine.com/medline/citation/17076675/Expression_of_natural_cytotoxicity_receptors_and_a2V_ATPase_on_peripheral_blood_NK_cell_subsets_in_women_with_recurrent_spontaneous_abortions_and_implantation_failures_ L2 - https://doi.org/10.1111/j.1600-0897.2006.00431.x DB - PRIME DP - Unbound Medicine ER -