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Detection of feline herpesvirus 1 DNA in skin biopsy specimens from cats with or without dermatitis.
J Am Vet Med Assoc. 2006 Nov 01; 229(9):1442-6.JA

Abstract

OBJECTIVE

To compare detection rates of feline herpesvirus 1 (FHV-1) DNA in skin biopsy specimens from cats with herpetic dermatitis, cats with nonherpetic dermatitis, and cats without dermatitis.

DESIGN

Prevalence survey. Animals-5 cats (9 biopsy specimens) with herpetic ulcerative dermatitis, 14 cats (17 biopsy specimens) with nonherpetic ulcerative dermatitis, and 8 cats (21 biopsy specimens) without clinically apparent skin lesions.

PROCEDURES

A single-phase PCR assay was used to detect FHV-1 DNA in biopsy specimens. Assay results were compared with results of histologic examination.

RESULTS

FHV-1 DNA was detected in all 9 biopsy specimens from the 5 cats with herpetic dermatitis and in 1 of 17 biopsy specimens from the 14 cats with nonherpetic dermatitis, but was not detected in any of the 21 biopsy specimens from the 8 cats without dermatitis. When results of histologic examination were used as the gold standard, sensitivity and specificity of the PCR assay were 100% and 95%, respectively.

CONCLUSIONS AND CLINICAL RELEVANCE

Results confirmed that FHV-1 DNA can be detected in the skin of cats with herpetic dermatitis and suggest that the virus may play a causative role in the disease. In addition, the PCR assay may be useful in confirming a diagnosis of herpetic dermatitis.

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Publisher Full Text

Authors+Show Affiliations

Holland JL
Departments of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Outerbridge CA
No affiliation info available
Affolter VK
No affiliation info available
Maggs DJ
No affiliation info available

MeSH

AnimalsCat DiseasesCatsDNA, ViralDermatitisDiagnosis, DifferentialFemaleHerpesviridaeHerpesviridae InfectionsMalePolymerase Chain ReactionPrevalenceSkinUnited States

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17078806

Citation

Holland, Jessica L., et al. "Detection of Feline Herpesvirus 1 DNA in Skin Biopsy Specimens From Cats With or Without Dermatitis." Journal of the American Veterinary Medical Association, vol. 229, no. 9, 2006, pp. 1442-6.
Holland JL, Outerbridge CA, Affolter VK, et al. Detection of feline herpesvirus 1 DNA in skin biopsy specimens from cats with or without dermatitis. J Am Vet Med Assoc. 2006;229(9):1442-6.
Holland, J. L., Outerbridge, C. A., Affolter, V. K., & Maggs, D. J. (2006). Detection of feline herpesvirus 1 DNA in skin biopsy specimens from cats with or without dermatitis. Journal of the American Veterinary Medical Association, 229(9), 1442-6.
Holland JL, et al. Detection of Feline Herpesvirus 1 DNA in Skin Biopsy Specimens From Cats With or Without Dermatitis. J Am Vet Med Assoc. 2006 Nov 1;229(9):1442-6. PubMed PMID: 17078806.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of feline herpesvirus 1 DNA in skin biopsy specimens from cats with or without dermatitis. AU - Holland,Jessica L, AU - Outerbridge,Catherine A, AU - Affolter,Verena K, AU - Maggs,David J, PY - 2006/11/3/pubmed PY - 2006/12/21/medline PY - 2006/11/3/entrez SP - 1442 EP - 6 JF - Journal of the American Veterinary Medical Association JO - J Am Vet Med Assoc VL - 229 IS - 9 N2 - OBJECTIVE: To compare detection rates of feline herpesvirus 1 (FHV-1) DNA in skin biopsy specimens from cats with herpetic dermatitis, cats with nonherpetic dermatitis, and cats without dermatitis. DESIGN: Prevalence survey. Animals-5 cats (9 biopsy specimens) with herpetic ulcerative dermatitis, 14 cats (17 biopsy specimens) with nonherpetic ulcerative dermatitis, and 8 cats (21 biopsy specimens) without clinically apparent skin lesions. PROCEDURES: A single-phase PCR assay was used to detect FHV-1 DNA in biopsy specimens. Assay results were compared with results of histologic examination. RESULTS: FHV-1 DNA was detected in all 9 biopsy specimens from the 5 cats with herpetic dermatitis and in 1 of 17 biopsy specimens from the 14 cats with nonherpetic dermatitis, but was not detected in any of the 21 biopsy specimens from the 8 cats without dermatitis. When results of histologic examination were used as the gold standard, sensitivity and specificity of the PCR assay were 100% and 95%, respectively. CONCLUSIONS AND CLINICAL RELEVANCE: Results confirmed that FHV-1 DNA can be detected in the skin of cats with herpetic dermatitis and suggest that the virus may play a causative role in the disease. In addition, the PCR assay may be useful in confirming a diagnosis of herpetic dermatitis. SN - 0003-1488 UR - https://www.unboundmedicine.com/medline/citation/17078806/Detection_of_feline_herpesvirus_1_DNA_in_skin_biopsy_specimens_from_cats_with_or_without_dermatitis_ L2 - https://avmajournals.avma.org/doi/10.2460/javma.229.9.1442?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -

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