Tags

Type your tag names separated by a space and hit enter

Miniaturized liquid core waveguide-based fluorimetric detection cell for capillary separation methods: application in CE of amino acids.
Electrophoresis. 2006 Dec; 27(23):4658-65.E

Abstract

A miniaturized post-column fluorimetric detection cell for capillary separation methods based on optical fibers and liquid core waveguides (LCWs) is described. The main part of the detection cell is a fused-silica capillary coated with Teflon AF serving as an LCW. The optical fibers are used both for coupling the excitation source with the detection domain in the LCW and for the axial fluorescence collection from the LCW end. The latter fiber is connected with a compact CCD spectrometer that serves for the rejection of the scattered excitation light and for the fluorescence signal detection. The proposed design offers a compact fluorescence detector for various microcolumn separation techniques without optical elements such as filters or objectives. Moreover, its construction and optical adjustment are very simple and the whole system is highly miniaturized. The function of the detection cell is demonstrated by CE of amino acids labelled by fluorescein-based tags. Separations of different standard amino acid mixtures and plasma samples are presented. The comparison of plasma amino acid levels of individuals being in good health with those of patients with inherited metabolic disorders is also shown.

Authors+Show Affiliations

Kostal V
Department of Analytical Chemistry, Faculty of Science, Palacky University, Olomouc, Czech Republic. kostal@iach.cz
Zeisbergerova M
No affiliation info available
Hrotekova Z
No affiliation info available
Slais K
No affiliation info available
Kahle V
No affiliation info available

MeSH

Amino AcidsElectrophoresis, CapillaryFiber Optic TechnologyHumansMiniaturizationOptical FibersSpectrometry, Fluorescence

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17080476

Citation

Kostal, Vratislav, et al. "Miniaturized Liquid Core Waveguide-based Fluorimetric Detection Cell for Capillary Separation Methods: Application in CE of Amino Acids." Electrophoresis, vol. 27, no. 23, 2006, pp. 4658-65.
Kostal V, Zeisbergerova M, Hrotekova Z, et al. Miniaturized liquid core waveguide-based fluorimetric detection cell for capillary separation methods: application in CE of amino acids. Electrophoresis. 2006;27(23):4658-65.
Kostal, V., Zeisbergerova, M., Hrotekova, Z., Slais, K., & Kahle, V. (2006). Miniaturized liquid core waveguide-based fluorimetric detection cell for capillary separation methods: application in CE of amino acids. Electrophoresis, 27(23), 4658-65.
Kostal V, et al. Miniaturized Liquid Core Waveguide-based Fluorimetric Detection Cell for Capillary Separation Methods: Application in CE of Amino Acids. Electrophoresis. 2006;27(23):4658-65. PubMed PMID: 17080476.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Miniaturized liquid core waveguide-based fluorimetric detection cell for capillary separation methods: application in CE of amino acids. AU - Kostal,Vratislav, AU - Zeisbergerova,Marta, AU - Hrotekova,Zuzana, AU - Slais,Karel, AU - Kahle,Vladislav, PY - 2006/11/3/pubmed PY - 2007/3/14/medline PY - 2006/11/3/entrez SP - 4658 EP - 65 JF - Electrophoresis JO - Electrophoresis VL - 27 IS - 23 N2 - A miniaturized post-column fluorimetric detection cell for capillary separation methods based on optical fibers and liquid core waveguides (LCWs) is described. The main part of the detection cell is a fused-silica capillary coated with Teflon AF serving as an LCW. The optical fibers are used both for coupling the excitation source with the detection domain in the LCW and for the axial fluorescence collection from the LCW end. The latter fiber is connected with a compact CCD spectrometer that serves for the rejection of the scattered excitation light and for the fluorescence signal detection. The proposed design offers a compact fluorescence detector for various microcolumn separation techniques without optical elements such as filters or objectives. Moreover, its construction and optical adjustment are very simple and the whole system is highly miniaturized. The function of the detection cell is demonstrated by CE of amino acids labelled by fluorescein-based tags. Separations of different standard amino acid mixtures and plasma samples are presented. The comparison of plasma amino acid levels of individuals being in good health with those of patients with inherited metabolic disorders is also shown. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/17080476/Miniaturized_liquid_core_waveguide_based_fluorimetric_detection_cell_for_capillary_separation_methods:_application_in_CE_of_amino_acids_ L2 - https://doi.org/10.1002/elps.200600222 DB - PRIME DP - Unbound Medicine ER -