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Comparison between micellar liquid chromatography and capillary zone electrophoresis for the determination of hydrophobic basic drugs in pharmaceutical preparations.
Biomed Chromatogr. 2007 Jan; 21(1):21-8.BC

Abstract

The determination of highly hydrophobic basic compounds by means of conventional reversed-phase liquid chromatographic methods has several drawbacks. Owing to the characteristics of micellar liquid chromatography (MLC) and capillary electrophoresis (CE), these techniques could be advantageous alternatives to reversed-phase chromatographic methods for the determination of these kinds of compounds. The objective of this study was to develop and compare MLC and CE methods for the determination of antipsychotic basic drugs (amitryptiline, haloperidol, perphenazine and thioridazine) in pharmaceutical preparations. The chromatographic determination of the analytes was performed on a Kromasil C(18) analytical column; the mobile phase was 0.04 m cetyltrimethylammonium bromide (CTAB), at pH 3, containing 5% 1-butanol, at a flow rate of 1 mL/min. The CE separation was performed in a fused-silica capillary with a 50 mm tris-(hydroxymethyl)-aminomethane buffer, pH 7, at an applied voltage of 20 kV, using barbital as internal stardard. The proposed methods are suitable for a reliable quantitation of these compounds in the commercial tablets and drops in terms of accuracy and precision and require a very simple pre-treatment of the samples. By comparing the performance characteristics and experimental details of the MLC and CE methods we conclude that CE seems to be slightly better than MLC in the determination of highly hydrophobic compounds in pharmaceuticals in terms of resolution and economy, taking into account that the limits of detection are not a handicap in pharmaceutical samples.

Authors+Show Affiliations

Torres-Cartas S
Departmento de Quimica Analita, Facultad de Farmacia, Universidad de Valencia, Valencia, Spain.
Martín-Biosca Y
No affiliation info available
Sagrado S
No affiliation info available
Villanueva-Camañas RM
No affiliation info available
Medina-Hernández MJ
No affiliation info available

MeSH

Antipsychotic AgentsCalibrationChromatography, LiquidElectrophoresis, CapillaryHydrogen-Ion ConcentrationHydrophobic and Hydrophilic InteractionsPharmaceutical PreparationsReference StandardsReproducibility of ResultsSensitivity and Specificity

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17080499

Citation

Torres-Cartas, S, et al. "Comparison Between Micellar Liquid Chromatography and Capillary Zone Electrophoresis for the Determination of Hydrophobic Basic Drugs in Pharmaceutical Preparations." Biomedical Chromatography : BMC, vol. 21, no. 1, 2007, pp. 21-8.
Torres-Cartas S, Martín-Biosca Y, Sagrado S, et al. Comparison between micellar liquid chromatography and capillary zone electrophoresis for the determination of hydrophobic basic drugs in pharmaceutical preparations. Biomed Chromatogr. 2007;21(1):21-8.
Torres-Cartas, S., Martín-Biosca, Y., Sagrado, S., Villanueva-Camañas, R. M., & Medina-Hernández, M. J. (2007). Comparison between micellar liquid chromatography and capillary zone electrophoresis for the determination of hydrophobic basic drugs in pharmaceutical preparations. Biomedical Chromatography : BMC, 21(1), 21-8.
Torres-Cartas S, et al. Comparison Between Micellar Liquid Chromatography and Capillary Zone Electrophoresis for the Determination of Hydrophobic Basic Drugs in Pharmaceutical Preparations. Biomed Chromatogr. 2007;21(1):21-8. PubMed PMID: 17080499.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison between micellar liquid chromatography and capillary zone electrophoresis for the determination of hydrophobic basic drugs in pharmaceutical preparations. AU - Torres-Cartas,S, AU - Martín-Biosca,Y, AU - Sagrado,S, AU - Villanueva-Camañas,R M, AU - Medina-Hernández,M J, PY - 2006/11/3/pubmed PY - 2007/5/5/medline PY - 2006/11/3/entrez SP - 21 EP - 8 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 21 IS - 1 N2 - The determination of highly hydrophobic basic compounds by means of conventional reversed-phase liquid chromatographic methods has several drawbacks. Owing to the characteristics of micellar liquid chromatography (MLC) and capillary electrophoresis (CE), these techniques could be advantageous alternatives to reversed-phase chromatographic methods for the determination of these kinds of compounds. The objective of this study was to develop and compare MLC and CE methods for the determination of antipsychotic basic drugs (amitryptiline, haloperidol, perphenazine and thioridazine) in pharmaceutical preparations. The chromatographic determination of the analytes was performed on a Kromasil C(18) analytical column; the mobile phase was 0.04 m cetyltrimethylammonium bromide (CTAB), at pH 3, containing 5% 1-butanol, at a flow rate of 1 mL/min. The CE separation was performed in a fused-silica capillary with a 50 mm tris-(hydroxymethyl)-aminomethane buffer, pH 7, at an applied voltage of 20 kV, using barbital as internal stardard. The proposed methods are suitable for a reliable quantitation of these compounds in the commercial tablets and drops in terms of accuracy and precision and require a very simple pre-treatment of the samples. By comparing the performance characteristics and experimental details of the MLC and CE methods we conclude that CE seems to be slightly better than MLC in the determination of highly hydrophobic compounds in pharmaceuticals in terms of resolution and economy, taking into account that the limits of detection are not a handicap in pharmaceutical samples. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/17080499/Comparison_between_micellar_liquid_chromatography_and_capillary_zone_electrophoresis_for_the_determination_of_hydrophobic_basic_drugs_in_pharmaceutical_preparations_ L2 - https://doi.org/10.1002/bmc.710 DB - PRIME DP - Unbound Medicine ER -