Tags

Type your tag names separated by a space and hit enter

Narrow-spectrum staining pattern of Pityrosporum.
J Cutan Pathol. 2006 Nov; 33(11):731-4.JC

Abstract

BACKGROUND

Broad-spectrum fungal stains are used to detect fungal organisms, but narrow-spectrum stains can assist in fungal differential diagnosis. These stains include mucicarmine and Fontana-Masson (FM) for Cryptococcus, Alcian Blue for Cryptococcus and Blastomyces, Congo Red for Blastomyces and Coccidioides, and Ziehl-Neelsen for some examples of Blastomyces and Histoplasma. Pityrosporum is increasingly being recognized as a pathogen capable of significant cutaneous and systemic infections, but the narrow-spectrum staining pattern of Pityrosporum has not yet been systematically studied and reported.

METHODS

Paraffin-embedded archival tissue from 10 skin biopsies containing Pityrosporum was stained with Alcian Blue, Congo Red, Ziehl-Neelsen acid-fast stain, and FM stain. In addition to the 60-min staining time, a modified FM stain with 15-, 30-, and 45-min staining times was also performed.

RESULTS

All cases stained positive with Alcian Blue, Congo Red, and FM stains and negative with the Ziehl-Neelsen stain. The modified FM stain with the 15-min staining time showed significant staining in more than 50% of the cases.

DISCUSSION

The narrow-spectrum staining pattern of Pityrosporum distinguishes it from Cryptococcus, Blastomyces, Candida, Histoplasma, and Coccidioides.

Links

Aggregator Full Text

Authors+Show Affiliations

Veerappan R
Department of Pathology, East Tennessee State University, Johnson City, TN, USA.
Miller LE
No affiliation info available
Sosinski C
No affiliation info available
Youngberg GA
No affiliation info available

MeSH

BiopsyBlastomycesBlastomycosisCandidaCandidiasis, CutaneousCoccidioidesCoccidioidomycosisColoring AgentsCryptococcosisCryptococcusDermatomycosesDiagnosis, DifferentialHistocytochemistryHistoplasmaHistoplasmosisHumansMalasseziaMycologySkin

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17083692

Citation

Veerappan, Ranjitha, et al. "Narrow-spectrum Staining Pattern of Pityrosporum." Journal of Cutaneous Pathology, vol. 33, no. 11, 2006, pp. 731-4.
Veerappan R, Miller LE, Sosinski C, et al. Narrow-spectrum staining pattern of Pityrosporum. J Cutan Pathol. 2006;33(11):731-4.
Veerappan, R., Miller, L. E., Sosinski, C., & Youngberg, G. A. (2006). Narrow-spectrum staining pattern of Pityrosporum. Journal of Cutaneous Pathology, 33(11), 731-4.
Veerappan R, et al. Narrow-spectrum Staining Pattern of Pityrosporum. J Cutan Pathol. 2006;33(11):731-4. PubMed PMID: 17083692.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Narrow-spectrum staining pattern of Pityrosporum. AU - Veerappan,Ranjitha, AU - Miller,Lou Ellen, AU - Sosinski,Christina, AU - Youngberg,George A, PY - 2006/11/7/pubmed PY - 2007/1/11/medline PY - 2006/11/7/entrez SP - 731 EP - 4 JF - Journal of cutaneous pathology JO - J Cutan Pathol VL - 33 IS - 11 N2 - BACKGROUND: Broad-spectrum fungal stains are used to detect fungal organisms, but narrow-spectrum stains can assist in fungal differential diagnosis. These stains include mucicarmine and Fontana-Masson (FM) for Cryptococcus, Alcian Blue for Cryptococcus and Blastomyces, Congo Red for Blastomyces and Coccidioides, and Ziehl-Neelsen for some examples of Blastomyces and Histoplasma. Pityrosporum is increasingly being recognized as a pathogen capable of significant cutaneous and systemic infections, but the narrow-spectrum staining pattern of Pityrosporum has not yet been systematically studied and reported. METHODS: Paraffin-embedded archival tissue from 10 skin biopsies containing Pityrosporum was stained with Alcian Blue, Congo Red, Ziehl-Neelsen acid-fast stain, and FM stain. In addition to the 60-min staining time, a modified FM stain with 15-, 30-, and 45-min staining times was also performed. RESULTS: All cases stained positive with Alcian Blue, Congo Red, and FM stains and negative with the Ziehl-Neelsen stain. The modified FM stain with the 15-min staining time showed significant staining in more than 50% of the cases. DISCUSSION: The narrow-spectrum staining pattern of Pityrosporum distinguishes it from Cryptococcus, Blastomyces, Candida, Histoplasma, and Coccidioides. SN - 0303-6987 UR - https://www.unboundmedicine.com/medline/citation/17083692/Narrow_spectrum_staining_pattern_of_Pityrosporum_ L2 - https://doi.org/10.1111/j.1600-0560.2006.00537.x DB - PRIME DP - Unbound Medicine ER -