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Diagnosis of American foulbrood in honey bees: a synthesis and proposed analytical protocols.
Lett Appl Microbiol. 2006 Dec; 43(6):583-90.LA

Abstract

Worldwide, American foulbrood (AFB) is the most devastating bacterial disease of the honey bee (Apis mellifera). Because the distinction between AFB and powdery scale disease is no longer considered valid, the pathogenic agent has recently been reclassified as one species Paenibacillus larvae, eliminating the subspecies designations Paenibacillus larvae subsp. larvae and Paenibacillus larvae subsp. pulvifaciens. The creamy or dark brown, glue-like larval remains of infected larvae continue to provide the most obvious clinical symptom of AFB, although it is not conclusive. Several sensitive and selective culture media are available for isolation of this spore-forming bacterium, with the type of samples that may be utilized for detection of the organism being further expanded. PCR methods for identification and genotyping of the pathogen have now been extensively developed. Nevertheless, biochemical profiling, bacteriophage sensitivity, immunotechniques and microscopy of suspect bacterial strains are entirely adequate for routine identification purposes.

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Authors+Show Affiliations

de Graaf DC
Laboratory of Zoophysiolgy, Ghent University, Ghent, Belgium. dirk.degraaf@ugent.be
Alippi AM
No affiliation info available
Brown M
No affiliation info available
Evans JD
No affiliation info available
Feldlaufer M
No affiliation info available
Gregorc A
No affiliation info available
Hornitzky M
No affiliation info available
Pernal SF
No affiliation info available
Schuch DM
No affiliation info available
Titera D
No affiliation info available
Tomkies V
No affiliation info available
Ritter W
No affiliation info available

MeSH

AnimalsBacillusBacterial Typing TechniquesBeesDNA, BacterialHoneyLarvaSpores, Bacterial

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

17083701

Citation

de Graaf, D C., et al. "Diagnosis of American Foulbrood in Honey Bees: a Synthesis and Proposed Analytical Protocols." Letters in Applied Microbiology, vol. 43, no. 6, 2006, pp. 583-90.
de Graaf DC, Alippi AM, Brown M, et al. Diagnosis of American foulbrood in honey bees: a synthesis and proposed analytical protocols. Lett Appl Microbiol. 2006;43(6):583-90.
de Graaf, D. C., Alippi, A. M., Brown, M., Evans, J. D., Feldlaufer, M., Gregorc, A., Hornitzky, M., Pernal, S. F., Schuch, D. M., Titera, D., Tomkies, V., & Ritter, W. (2006). Diagnosis of American foulbrood in honey bees: a synthesis and proposed analytical protocols. Letters in Applied Microbiology, 43(6), 583-90.
de Graaf DC, et al. Diagnosis of American Foulbrood in Honey Bees: a Synthesis and Proposed Analytical Protocols. Lett Appl Microbiol. 2006;43(6):583-90. PubMed PMID: 17083701.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diagnosis of American foulbrood in honey bees: a synthesis and proposed analytical protocols. AU - de Graaf,D C, AU - Alippi,A M, AU - Brown,M, AU - Evans,J D, AU - Feldlaufer,M, AU - Gregorc,A, AU - Hornitzky,M, AU - Pernal,S F, AU - Schuch,D M T, AU - Titera,D, AU - Tomkies,V, AU - Ritter,W, PY - 2006/11/7/pubmed PY - 2007/2/28/medline PY - 2006/11/7/entrez SP - 583 EP - 90 JF - Letters in applied microbiology JO - Lett Appl Microbiol VL - 43 IS - 6 N2 - Worldwide, American foulbrood (AFB) is the most devastating bacterial disease of the honey bee (Apis mellifera). Because the distinction between AFB and powdery scale disease is no longer considered valid, the pathogenic agent has recently been reclassified as one species Paenibacillus larvae, eliminating the subspecies designations Paenibacillus larvae subsp. larvae and Paenibacillus larvae subsp. pulvifaciens. The creamy or dark brown, glue-like larval remains of infected larvae continue to provide the most obvious clinical symptom of AFB, although it is not conclusive. Several sensitive and selective culture media are available for isolation of this spore-forming bacterium, with the type of samples that may be utilized for detection of the organism being further expanded. PCR methods for identification and genotyping of the pathogen have now been extensively developed. Nevertheless, biochemical profiling, bacteriophage sensitivity, immunotechniques and microscopy of suspect bacterial strains are entirely adequate for routine identification purposes. SN - 0266-8254 UR - https://www.unboundmedicine.com/medline/citation/17083701/Diagnosis_of_American_foulbrood_in_honey_bees:_a_synthesis_and_proposed_analytical_protocols_ L2 - https://doi.org/10.1111/j.1472-765X.2006.02057.x DB - PRIME DP - Unbound Medicine ER -