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Reduced allergenicity of tomato fruits harvested from Lyc e 1-silenced transgenic tomato plants.
J Allergy Clin Immunol. 2006 Nov; 118(5):1176-83.JA

Abstract

BACKGROUND

Profilin is a small actin-binding protein that contributes to the allergenic potency of many fruits and vegetables, including tomato. Two highly similar genes encoding tomato profilin have been isolated and designated as allergen Lyc e 1.01 and Lyc e 1.02.

OBJECTIVE

The aim of the study was to generate profilin-reduced hypoallergenic tomato fruits by silencing of both genes in transgenic tomato plants by means of RNA interference (RNAi).

METHODS

The efficiency of gene silencing was documented by means of Northern blotting, immunoblotting, and skin prick testing.

RESULTS

Quantification of the remaining protein revealed that profilin accumulation in transgenic fruits was decreased 10-fold compared with that seen in untransformed controls. This decrease was sufficient to cause a reduced allergenic reactivity in patients with tomato allergy, as determined with skin prick tests. Because most patients with tomato allergy are not monosensitized to profilin, the IgE reactivity to the profilin-silenced tomato fruits in vivo varied widely between individuals tested.

CONCLUSION

We could demonstrate the efficient silencing of both profilin genes in transgenic tomato plants using RNAi. This resulted in Lyc e 1-diminished tomato fruits, providing proof of concept and demonstrating that RNAi can be used to design allergen-reduced food. However, simultaneous silencing of multiple allergens will be required to design hypoallergenic tomatoes.

CLINICAL IMPLICATIONS

Our findings demonstrate the feasibility of creating low-allergenic food by using RNAi. This concept constitutes a novel approach to allergen avoidance.

Authors+Show Affiliations

Department of Biochemistry, Friedrich-Alexander University Erlangen-Nuremberg, Erlangen, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17088146

Citation

Le, Lien Quynh, et al. "Reduced Allergenicity of Tomato Fruits Harvested From Lyc E 1-silenced Transgenic Tomato Plants." The Journal of Allergy and Clinical Immunology, vol. 118, no. 5, 2006, pp. 1176-83.
Le LQ, Mahler V, Lorenz Y, et al. Reduced allergenicity of tomato fruits harvested from Lyc e 1-silenced transgenic tomato plants. J Allergy Clin Immunol. 2006;118(5):1176-83.
Le, L. Q., Mahler, V., Lorenz, Y., Scheurer, S., Biemelt, S., Vieths, S., & Sonnewald, U. (2006). Reduced allergenicity of tomato fruits harvested from Lyc e 1-silenced transgenic tomato plants. The Journal of Allergy and Clinical Immunology, 118(5), 1176-83.
Le LQ, et al. Reduced Allergenicity of Tomato Fruits Harvested From Lyc E 1-silenced Transgenic Tomato Plants. J Allergy Clin Immunol. 2006;118(5):1176-83. PubMed PMID: 17088146.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Reduced allergenicity of tomato fruits harvested from Lyc e 1-silenced transgenic tomato plants. AU - Le,Lien Quynh, AU - Mahler,Vera, AU - Lorenz,Yvonne, AU - Scheurer,Stephan, AU - Biemelt,Sophia, AU - Vieths,Stefan, AU - Sonnewald,Uwe, Y1 - 2006/09/08/ PY - 2005/12/20/received PY - 2006/06/27/revised PY - 2006/06/27/accepted PY - 2006/11/8/pubmed PY - 2006/12/16/medline PY - 2006/11/8/entrez SP - 1176 EP - 83 JF - The Journal of allergy and clinical immunology JO - J Allergy Clin Immunol VL - 118 IS - 5 N2 - BACKGROUND: Profilin is a small actin-binding protein that contributes to the allergenic potency of many fruits and vegetables, including tomato. Two highly similar genes encoding tomato profilin have been isolated and designated as allergen Lyc e 1.01 and Lyc e 1.02. OBJECTIVE: The aim of the study was to generate profilin-reduced hypoallergenic tomato fruits by silencing of both genes in transgenic tomato plants by means of RNA interference (RNAi). METHODS: The efficiency of gene silencing was documented by means of Northern blotting, immunoblotting, and skin prick testing. RESULTS: Quantification of the remaining protein revealed that profilin accumulation in transgenic fruits was decreased 10-fold compared with that seen in untransformed controls. This decrease was sufficient to cause a reduced allergenic reactivity in patients with tomato allergy, as determined with skin prick tests. Because most patients with tomato allergy are not monosensitized to profilin, the IgE reactivity to the profilin-silenced tomato fruits in vivo varied widely between individuals tested. CONCLUSION: We could demonstrate the efficient silencing of both profilin genes in transgenic tomato plants using RNAi. This resulted in Lyc e 1-diminished tomato fruits, providing proof of concept and demonstrating that RNAi can be used to design allergen-reduced food. However, simultaneous silencing of multiple allergens will be required to design hypoallergenic tomatoes. CLINICAL IMPLICATIONS: Our findings demonstrate the feasibility of creating low-allergenic food by using RNAi. This concept constitutes a novel approach to allergen avoidance. SN - 0091-6749 UR - https://www.unboundmedicine.com/medline/citation/17088146/Reduced_allergenicity_of_tomato_fruits_harvested_from_Lyc_e_1_silenced_transgenic_tomato_plants_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0091-6749(06)01384-4 DB - PRIME DP - Unbound Medicine ER -