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Pituitary hormone mRNA expression in European sea bass Dicentrarchus labrax in seawater and following acclimation to fresh water.
J Endocrinol. 2006 Nov; 191(2):473-80.JE

Abstract

The mRNA expression of pituitary prolactin (prl), growth hormone (gh), somatolactin (sl), proopiomelanocortin (pomc), and gonadotropins (gthI and gthII) was quantified by real-time PCR, in sea bass, Dicentrarchus labrax, adapted for 1 month to seawater (SW) or freshwater (FW). In addition, IGF-I (igfI) mRNA expression in liver and branchial Na+/K+ -ATPase activity were determined. L17 ribosomal protein (rpL17) and elongation factor 1alpha (ef1alpha) were validated as reference genes in real-time PCR in the experimental context. The real-time PCR assays were validated for the different hormone genes considered. Expression of pituitary pomc, gthI, gthII, gh, and liver igfI was not significantly different between FW and SW fish. Pituitary prlwas 4.5-foldhigher in FWthan in SW, whereas pituitary sl was 1.8-fold higher in SW- compared with FW-adapted fish. Gill Na+/K+ -ATPase specific activity was 2.3-fold higher in FW sea bass compared with SW fish. Plasma cortisol levels were 6.5-fold lower in SW- than in FW-adapted specimens. The results are discussed in relation to the osmoregulatory strategy of this euryhaline SW species, which displays features that do not fit present models based on salmonids and FWeuryhaline teleosts.

Authors+Show Affiliations

Adaptation Ecophysiologique et Ontogenèse, UMR 5171, GPIA, Université Montpellier II, Place Eugène Bataillon, CC092, 34095 Montpellier Cedex 05, France. varsamos@univ-montp2.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

17088417

Citation

Varsamos, Stamatis, et al. "Pituitary Hormone mRNA Expression in European Sea Bass Dicentrarchus Labrax in Seawater and Following Acclimation to Fresh Water." The Journal of Endocrinology, vol. 191, no. 2, 2006, pp. 473-80.
Varsamos S, Xuereb B, Commes T, et al. Pituitary hormone mRNA expression in European sea bass Dicentrarchus labrax in seawater and following acclimation to fresh water. J Endocrinol. 2006;191(2):473-80.
Varsamos, S., Xuereb, B., Commes, T., Flik, G., & Spanings-Pierrot, C. (2006). Pituitary hormone mRNA expression in European sea bass Dicentrarchus labrax in seawater and following acclimation to fresh water. The Journal of Endocrinology, 191(2), 473-80.
Varsamos S, et al. Pituitary Hormone mRNA Expression in European Sea Bass Dicentrarchus Labrax in Seawater and Following Acclimation to Fresh Water. J Endocrinol. 2006;191(2):473-80. PubMed PMID: 17088417.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pituitary hormone mRNA expression in European sea bass Dicentrarchus labrax in seawater and following acclimation to fresh water. AU - Varsamos,Stamatis, AU - Xuereb,Benoît, AU - Commes,Thérèse, AU - Flik,Gert, AU - Spanings-Pierrot,Céline, PY - 2006/11/8/pubmed PY - 2007/3/6/medline PY - 2006/11/8/entrez SP - 473 EP - 80 JF - The Journal of endocrinology JO - J Endocrinol VL - 191 IS - 2 N2 - The mRNA expression of pituitary prolactin (prl), growth hormone (gh), somatolactin (sl), proopiomelanocortin (pomc), and gonadotropins (gthI and gthII) was quantified by real-time PCR, in sea bass, Dicentrarchus labrax, adapted for 1 month to seawater (SW) or freshwater (FW). In addition, IGF-I (igfI) mRNA expression in liver and branchial Na+/K+ -ATPase activity were determined. L17 ribosomal protein (rpL17) and elongation factor 1alpha (ef1alpha) were validated as reference genes in real-time PCR in the experimental context. The real-time PCR assays were validated for the different hormone genes considered. Expression of pituitary pomc, gthI, gthII, gh, and liver igfI was not significantly different between FW and SW fish. Pituitary prlwas 4.5-foldhigher in FWthan in SW, whereas pituitary sl was 1.8-fold higher in SW- compared with FW-adapted fish. Gill Na+/K+ -ATPase specific activity was 2.3-fold higher in FW sea bass compared with SW fish. Plasma cortisol levels were 6.5-fold lower in SW- than in FW-adapted specimens. The results are discussed in relation to the osmoregulatory strategy of this euryhaline SW species, which displays features that do not fit present models based on salmonids and FWeuryhaline teleosts. SN - 0022-0795 UR - https://www.unboundmedicine.com/medline/citation/17088417/Pituitary_hormone_mRNA_expression_in_European_sea_bass_Dicentrarchus_labrax_in_seawater_and_following_acclimation_to_fresh_water_ DB - PRIME DP - Unbound Medicine ER -