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Uptake and metabolism of hydroxycinnamic acids (chlorogenic, caffeic, and ferulic acids) by HepG2 cells as a model of the human liver.
J Agric Food Chem. 2006 Nov 15; 54(23):8724-32.JA

Abstract

Hydroxycinnamic acids are antioxidant polyphenols common in the human diet, although their potential health benefits depend on their bioavailability. To study the hepatic uptake and metabolism, human hepatoma HepG2 cells were incubated for 2 and 18 h with caffeic, ferulic, and chlorogenic acids. Moderate uptake of caffeic and ferulic acids was observed versus a low absorption of chlorogenic acid, where esterification of the caffeic acid moiety markedly reduced its absorption. Methylation was the preferential pathway for caffeic acid metabolism, along with glucuronidation and sulfation, while ferulic acid generated glucuronides as the only metabolites. Ferulic acid appeared to be more slowly taken up and metabolized by HepG2 cells than caffeic acid, with 73% and 64% of the free, nonmetabolized molecules detected in the culture medium after 18 h, respectively. In conclusion, hydroxycinnamic acids can be metabolized by the liver as suggested by the results obtained using HepG2 cells as a hepatic model system.

Authors+Show Affiliations

Department of Metabolism and Nutrition, Instituto del Frío (CSIC), C/José Antonio Novais, 10 Ciudad Universitaria, E-28040 Madrid, Spain.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17090113

Citation

Mateos, Raquel, et al. "Uptake and Metabolism of Hydroxycinnamic Acids (chlorogenic, Caffeic, and Ferulic Acids) By HepG2 Cells as a Model of the Human Liver." Journal of Agricultural and Food Chemistry, vol. 54, no. 23, 2006, pp. 8724-32.
Mateos R, Goya L, Bravo L. Uptake and metabolism of hydroxycinnamic acids (chlorogenic, caffeic, and ferulic acids) by HepG2 cells as a model of the human liver. J Agric Food Chem. 2006;54(23):8724-32.
Mateos, R., Goya, L., & Bravo, L. (2006). Uptake and metabolism of hydroxycinnamic acids (chlorogenic, caffeic, and ferulic acids) by HepG2 cells as a model of the human liver. Journal of Agricultural and Food Chemistry, 54(23), 8724-32.
Mateos R, Goya L, Bravo L. Uptake and Metabolism of Hydroxycinnamic Acids (chlorogenic, Caffeic, and Ferulic Acids) By HepG2 Cells as a Model of the Human Liver. J Agric Food Chem. 2006 Nov 15;54(23):8724-32. PubMed PMID: 17090113.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Uptake and metabolism of hydroxycinnamic acids (chlorogenic, caffeic, and ferulic acids) by HepG2 cells as a model of the human liver. AU - Mateos,Raquel, AU - Goya,Luis, AU - Bravo,Laura, PY - 2006/11/9/pubmed PY - 2007/1/11/medline PY - 2006/11/9/entrez SP - 8724 EP - 32 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 54 IS - 23 N2 - Hydroxycinnamic acids are antioxidant polyphenols common in the human diet, although their potential health benefits depend on their bioavailability. To study the hepatic uptake and metabolism, human hepatoma HepG2 cells were incubated for 2 and 18 h with caffeic, ferulic, and chlorogenic acids. Moderate uptake of caffeic and ferulic acids was observed versus a low absorption of chlorogenic acid, where esterification of the caffeic acid moiety markedly reduced its absorption. Methylation was the preferential pathway for caffeic acid metabolism, along with glucuronidation and sulfation, while ferulic acid generated glucuronides as the only metabolites. Ferulic acid appeared to be more slowly taken up and metabolized by HepG2 cells than caffeic acid, with 73% and 64% of the free, nonmetabolized molecules detected in the culture medium after 18 h, respectively. In conclusion, hydroxycinnamic acids can be metabolized by the liver as suggested by the results obtained using HepG2 cells as a hepatic model system. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/17090113/Uptake_and_metabolism_of_hydroxycinnamic_acids__chlorogenic_caffeic_and_ferulic_acids__by_HepG2_cells_as_a_model_of_the_human_liver_ L2 - https://doi.org/10.1021/jf061664g DB - PRIME DP - Unbound Medicine ER -