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Dietary fatty acids make a rapid and substantial contribution to VLDL-triacylglycerol in the fed state.
Am J Physiol Endocrinol Metab. 2007 Mar; 292(3):E732-9.AJ

Abstract

Exaggerated postprandial lipemia is associated with coronary heart disease and type II diabetes, yet few studies have examined the effect of sequential meals on lipoprotein metabolism. We have used 13C-labeled fatty acids to trace the incorporation of fatty acid derived from a meal into apolipoprotein B-100 (apoB-100)-containing lipoproteins and plasma nonesterified fatty acids (NEFA) following two consecutive meals. Healthy volunteers (n=8) were given breakfast labeled with [1-(13)C]palmitic acid, eicosapentaenoic acid, and docosahexaenoic acid, followed 5 h later by lunch containing [1-(13)C]oleic acid. Blood samples were taken over a 9-h period. ApoB-100-containing lipoproteins were isolated by immunoaffinity chromatography. Chylomicron-triacylglycerol (TG) concentrations peaked at 195 min following breakfast but at 75 min following lunch (P<0.001). VLDL-TG concentrations, in contrast, rose to a broad peak after breakfast and then fell steadily after lunch. Breakfast markers followed chylomicron-TG concentrations and appeared in plasma NEFA with a similar profile, whereas [1-(13)C]oleic acid peaked 2 h after lunch in plasma TG and NEFA. Breakfast markers appeared steadily in VLDL, peaking 1-3 h after lunch, whereas [1-(13)C]oleic acid was still accumulating in VLDL at 9 h. Around 17% of VLDL-TG originated from recent dietary fat 5 h after breakfast, and around 40% at the end of the experiment. We conclude that there is rapid flux of fatty acids from the diet into endogenous pools. Further study of these processes may open up new targets for intervention to reduce VLDL-TG concentrations and postprandial lipemia.

Authors+Show Affiliations

Oxford Centre for Diabetes, Endocrinology and Metabolism, University of Oxford, Churchill Hospital, Oxford OX3 7LJ, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17090753

Citation

Heath, Richard B., et al. "Dietary Fatty Acids Make a Rapid and Substantial Contribution to VLDL-triacylglycerol in the Fed State." American Journal of Physiology. Endocrinology and Metabolism, vol. 292, no. 3, 2007, pp. E732-9.
Heath RB, Karpe F, Milne RW, et al. Dietary fatty acids make a rapid and substantial contribution to VLDL-triacylglycerol in the fed state. Am J Physiol Endocrinol Metab. 2007;292(3):E732-9.
Heath, R. B., Karpe, F., Milne, R. W., Burdge, G. C., Wootton, S. A., & Frayn, K. N. (2007). Dietary fatty acids make a rapid and substantial contribution to VLDL-triacylglycerol in the fed state. American Journal of Physiology. Endocrinology and Metabolism, 292(3), E732-9.
Heath RB, et al. Dietary Fatty Acids Make a Rapid and Substantial Contribution to VLDL-triacylglycerol in the Fed State. Am J Physiol Endocrinol Metab. 2007;292(3):E732-9. PubMed PMID: 17090753.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary fatty acids make a rapid and substantial contribution to VLDL-triacylglycerol in the fed state. AU - Heath,Richard B, AU - Karpe,Fredrik, AU - Milne,Ross W, AU - Burdge,Graham C, AU - Wootton,Stephen A, AU - Frayn,Keith N, Y1 - 2006/11/07/ PY - 2006/11/9/pubmed PY - 2007/4/17/medline PY - 2006/11/9/entrez SP - E732 EP - 9 JF - American journal of physiology. Endocrinology and metabolism JO - Am. J. Physiol. Endocrinol. Metab. VL - 292 IS - 3 N2 - Exaggerated postprandial lipemia is associated with coronary heart disease and type II diabetes, yet few studies have examined the effect of sequential meals on lipoprotein metabolism. We have used 13C-labeled fatty acids to trace the incorporation of fatty acid derived from a meal into apolipoprotein B-100 (apoB-100)-containing lipoproteins and plasma nonesterified fatty acids (NEFA) following two consecutive meals. Healthy volunteers (n=8) were given breakfast labeled with [1-(13)C]palmitic acid, eicosapentaenoic acid, and docosahexaenoic acid, followed 5 h later by lunch containing [1-(13)C]oleic acid. Blood samples were taken over a 9-h period. ApoB-100-containing lipoproteins were isolated by immunoaffinity chromatography. Chylomicron-triacylglycerol (TG) concentrations peaked at 195 min following breakfast but at 75 min following lunch (P<0.001). VLDL-TG concentrations, in contrast, rose to a broad peak after breakfast and then fell steadily after lunch. Breakfast markers followed chylomicron-TG concentrations and appeared in plasma NEFA with a similar profile, whereas [1-(13)C]oleic acid peaked 2 h after lunch in plasma TG and NEFA. Breakfast markers appeared steadily in VLDL, peaking 1-3 h after lunch, whereas [1-(13)C]oleic acid was still accumulating in VLDL at 9 h. Around 17% of VLDL-TG originated from recent dietary fat 5 h after breakfast, and around 40% at the end of the experiment. We conclude that there is rapid flux of fatty acids from the diet into endogenous pools. Further study of these processes may open up new targets for intervention to reduce VLDL-TG concentrations and postprandial lipemia. SN - 0193-1849 UR - https://www.unboundmedicine.com/medline/citation/17090753/Dietary_fatty_acids_make_a_rapid_and_substantial_contribution_to_VLDL_triacylglycerol_in_the_fed_state_ L2 - http://www.physiology.org/doi/full/10.1152/ajpendo.00409.2006?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -