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Effect of genetic variants of CCR2 and CCL2 on the natural history of HIV-1 infection: CCL2-2518GG is overrepresented in a cohort of Spanish HIV-1-infected subjects.
J Acquir Immune Defic Syndr. 2007 Feb 01; 44(2):132-8.JA

Abstract

BACKGROUND AND OBJECTIVES

Polymorphisms in the genes that encode for the CCR2 chemokine receptor and its natural ligand CCL2 have been shown to influence the natural history of HIV-1 infection, although data are inconsistent. Our aim was to determine whether functionally active CCR2 and CCL2 genetic variants influence the risk of infection and disease progression in a cohort of white Spaniards.

PATIENTS AND METHODS

This was a multicenter genetic association case-control study. Two single nucleotide polymorphisms (SNPs), V64I (G > A) of the CCR2 gene and -2518 (A > G) of the CCL2 gene, were assessed in 318 individuals: 73 HIV-1-infected long-term nonprogressors (LTNPs) of >16 years duration, 109 HIV-1-infected usual progressors (UPs), 36 heavily exposed to HIV-1 but uninfected individuals (EUs), and 100 control subjects. The distribution of the CCR5Delta32 allele was also assessed. Genotyping was performed using polymerase chain reaction (PCR) restriction fragment length polymorphisms (RFLPs) or PCR and automatic sequencing analysis methods on white blood cell DNA. Genotype and allele frequencies were compared by the chi2 test and the Fisher exact test.

RESULTS

CCR2 genotype distribution and allele frequencies showed nonsignificant differences between groups. The distribution of CCL2 alleles showed no significant differences between groups. HIV-1-infected individuals had, however, a significantly higher prevalence of the variant homozygous CCL2 GG genotype compared with EUs (P = 0.02). This result persisted when we studied only individuals with wild-type CCR5. Genotype and allele distribution of CCL2 was similar in HIV-1-infected UPs and LTNPs.

CONCLUSIONS

In our cohort of white Spaniards, homozygosity for the variant CCL2-2518GG genotype is overrepresented in HIV-1-infected subjects.

Authors+Show Affiliations

Department of Internal Medicine, Infectious Diseases and AIDS Research unit, Hospital Universitari de Tarragona Joan XXIII and Universitat Rovira i Virgili, Tarragona, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Multicenter Study
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17091019

Citation

Viladés, Consuelo, et al. "Effect of Genetic Variants of CCR2 and CCL2 On the Natural History of HIV-1 Infection: CCL2-2518GG Is Overrepresented in a Cohort of Spanish HIV-1-infected Subjects." Journal of Acquired Immune Deficiency Syndromes (1999), vol. 44, no. 2, 2007, pp. 132-8.
Viladés C, Broch M, Plana M, et al. Effect of genetic variants of CCR2 and CCL2 on the natural history of HIV-1 infection: CCL2-2518GG is overrepresented in a cohort of Spanish HIV-1-infected subjects. J Acquir Immune Defic Syndr. 2007;44(2):132-8.
Viladés, C., Broch, M., Plana, M., Domingo, P., Alonso-Villaverde, C., Pedrol, E., Knobel, H., Dalmau, D., Peraire, J., Gutiérrez, C., López, A., Sambeat, M. A., Olona, M., Garcia, F., Richart, C., Gatell, J. M., & Vidal, F. (2007). Effect of genetic variants of CCR2 and CCL2 on the natural history of HIV-1 infection: CCL2-2518GG is overrepresented in a cohort of Spanish HIV-1-infected subjects. Journal of Acquired Immune Deficiency Syndromes (1999), 44(2), 132-8.
Viladés C, et al. Effect of Genetic Variants of CCR2 and CCL2 On the Natural History of HIV-1 Infection: CCL2-2518GG Is Overrepresented in a Cohort of Spanish HIV-1-infected Subjects. J Acquir Immune Defic Syndr. 2007 Feb 1;44(2):132-8. PubMed PMID: 17091019.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of genetic variants of CCR2 and CCL2 on the natural history of HIV-1 infection: CCL2-2518GG is overrepresented in a cohort of Spanish HIV-1-infected subjects. AU - Viladés,Consuelo, AU - Broch,Montserrat, AU - Plana,Montserrat, AU - Domingo,Pere, AU - Alonso-Villaverde,Carlos, AU - Pedrol,Enric, AU - Knobel,Hernando, AU - Dalmau,David, AU - Peraire,Joaquim, AU - Gutiérrez,Cristina, AU - López,Anna, AU - Sambeat,Maria Antònia, AU - Olona,Montserrat, AU - Garcia,Felipe, AU - Richart,Cristóbal, AU - Gatell,Josep Maria, AU - Vidal,Francesc, AU - ,, PY - 2006/11/9/pubmed PY - 2007/3/21/medline PY - 2006/11/9/entrez SP - 132 EP - 8 JF - Journal of acquired immune deficiency syndromes (1999) JO - J. Acquir. Immune Defic. Syndr. VL - 44 IS - 2 N2 - BACKGROUND AND OBJECTIVES: Polymorphisms in the genes that encode for the CCR2 chemokine receptor and its natural ligand CCL2 have been shown to influence the natural history of HIV-1 infection, although data are inconsistent. Our aim was to determine whether functionally active CCR2 and CCL2 genetic variants influence the risk of infection and disease progression in a cohort of white Spaniards. PATIENTS AND METHODS: This was a multicenter genetic association case-control study. Two single nucleotide polymorphisms (SNPs), V64I (G > A) of the CCR2 gene and -2518 (A > G) of the CCL2 gene, were assessed in 318 individuals: 73 HIV-1-infected long-term nonprogressors (LTNPs) of >16 years duration, 109 HIV-1-infected usual progressors (UPs), 36 heavily exposed to HIV-1 but uninfected individuals (EUs), and 100 control subjects. The distribution of the CCR5Delta32 allele was also assessed. Genotyping was performed using polymerase chain reaction (PCR) restriction fragment length polymorphisms (RFLPs) or PCR and automatic sequencing analysis methods on white blood cell DNA. Genotype and allele frequencies were compared by the chi2 test and the Fisher exact test. RESULTS: CCR2 genotype distribution and allele frequencies showed nonsignificant differences between groups. The distribution of CCL2 alleles showed no significant differences between groups. HIV-1-infected individuals had, however, a significantly higher prevalence of the variant homozygous CCL2 GG genotype compared with EUs (P = 0.02). This result persisted when we studied only individuals with wild-type CCR5. Genotype and allele distribution of CCL2 was similar in HIV-1-infected UPs and LTNPs. CONCLUSIONS: In our cohort of white Spaniards, homozygosity for the variant CCL2-2518GG genotype is overrepresented in HIV-1-infected subjects. SN - 1525-4135 UR - https://www.unboundmedicine.com/medline/citation/17091019/Effect_of_genetic_variants_of_CCR2_and_CCL2_on_the_natural_history_of_HIV_1_infection:_CCL2_2518GG_is_overrepresented_in_a_cohort_of_Spanish_HIV_1_infected_subjects_ L2 - http://dx.doi.org/10.1097/QAI.0b013e31802b3147 DB - PRIME DP - Unbound Medicine ER -