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Effect of GnRH analogues on apoptosis and expression of Bcl-2, Bax, Fas and FasL proteins in endometrial epithelial cell cultures from patients with endometriosis and controls.
Hum Reprod. 2007 Mar; 22(3):644-53.HR

Abstract

BACKGROUND

Our purpose was to evaluate the effect of the GnRH agonist (GnRHa), leuprolide acetate (LA), and the GnRH antagonist (GnRHant), Antide, on apoptosis and expression of apoptosis-related proteins in endometrial epithelial cell (EEC) cultures from patients with endometriosis and controls (infertile women without endometriosis).

METHODS

Biopsy specimens of eutopic endometrium were obtained from 22 patients with endometriosis and from 14 women that served as controls. Apoptosis was examined in EEC after incubation with LA and Antide. Bax, Bcl-2, Fas and FasL expression was evaluated after exposure to LA, Antide or a combination of both. The percentage of apoptotic cells (%ApC) was assessed by the acridine orange-ethidium bromide technique, and protein expression was evaluated by western blot and immunocytochemistry.

RESULTS

LA 100 and 1000 ng/ml increased the %ApC in EEC from patients with endometriosis (both P < 0.05) and controls (p < 0.05 and P < 0.01, respectively). Antide 10(-5) M increased the %ApC in EEC from patients with endometriosis and controls (P < 0.01). In EEC from women with endometriosis, Bax expression increased after treatment with LA, Antide and LA + Antide (P < 0.05, P < 0.001 and P < 0.001), whereas Bcl-2 expression decreased after exposure to LA and Antide (P < 0.001 and P < 0.01). FasL expression increased after LA, Antide and LA + Antide treatments (P < 0.01, P < 0.001 and P < 0.01). No significant changes were observed on Fas expression.

CONCLUSIONS

GnRH analogues enhanced apoptosis in EEC, and this was accompanied by an increase in expression of the pro-apoptotic proteins Bax and FasL and a decrease in expression of the anti-apoptotic protein Bcl-2.

Authors+Show Affiliations

Instituto de Biología y Medicina Experimental, Hospital de Clínicas José de San Martín, Buenos Aires, Argentina. mabilotas@dna.uba.arNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17092985

Citation

Bilotas, M, et al. "Effect of GnRH Analogues On Apoptosis and Expression of Bcl-2, Bax, Fas and FasL Proteins in Endometrial Epithelial Cell Cultures From Patients With Endometriosis and Controls." Human Reproduction (Oxford, England), vol. 22, no. 3, 2007, pp. 644-53.
Bilotas M, Barañao RI, Buquet R, et al. Effect of GnRH analogues on apoptosis and expression of Bcl-2, Bax, Fas and FasL proteins in endometrial epithelial cell cultures from patients with endometriosis and controls. Hum Reprod. 2007;22(3):644-53.
Bilotas, M., Barañao, R. I., Buquet, R., Sueldo, C., Tesone, M., & Meresman, G. (2007). Effect of GnRH analogues on apoptosis and expression of Bcl-2, Bax, Fas and FasL proteins in endometrial epithelial cell cultures from patients with endometriosis and controls. Human Reproduction (Oxford, England), 22(3), 644-53.
Bilotas M, et al. Effect of GnRH Analogues On Apoptosis and Expression of Bcl-2, Bax, Fas and FasL Proteins in Endometrial Epithelial Cell Cultures From Patients With Endometriosis and Controls. Hum Reprod. 2007;22(3):644-53. PubMed PMID: 17092985.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of GnRH analogues on apoptosis and expression of Bcl-2, Bax, Fas and FasL proteins in endometrial epithelial cell cultures from patients with endometriosis and controls. AU - Bilotas,M, AU - Barañao,R I, AU - Buquet,R, AU - Sueldo,C, AU - Tesone,M, AU - Meresman,G, Y1 - 2006/11/08/ PY - 2006/11/10/pubmed PY - 2007/5/5/medline PY - 2006/11/10/entrez SP - 644 EP - 53 JF - Human reproduction (Oxford, England) JO - Hum. Reprod. VL - 22 IS - 3 N2 - BACKGROUND: Our purpose was to evaluate the effect of the GnRH agonist (GnRHa), leuprolide acetate (LA), and the GnRH antagonist (GnRHant), Antide, on apoptosis and expression of apoptosis-related proteins in endometrial epithelial cell (EEC) cultures from patients with endometriosis and controls (infertile women without endometriosis). METHODS: Biopsy specimens of eutopic endometrium were obtained from 22 patients with endometriosis and from 14 women that served as controls. Apoptosis was examined in EEC after incubation with LA and Antide. Bax, Bcl-2, Fas and FasL expression was evaluated after exposure to LA, Antide or a combination of both. The percentage of apoptotic cells (%ApC) was assessed by the acridine orange-ethidium bromide technique, and protein expression was evaluated by western blot and immunocytochemistry. RESULTS: LA 100 and 1000 ng/ml increased the %ApC in EEC from patients with endometriosis (both P < 0.05) and controls (p < 0.05 and P < 0.01, respectively). Antide 10(-5) M increased the %ApC in EEC from patients with endometriosis and controls (P < 0.01). In EEC from women with endometriosis, Bax expression increased after treatment with LA, Antide and LA + Antide (P < 0.05, P < 0.001 and P < 0.001), whereas Bcl-2 expression decreased after exposure to LA and Antide (P < 0.001 and P < 0.01). FasL expression increased after LA, Antide and LA + Antide treatments (P < 0.01, P < 0.001 and P < 0.01). No significant changes were observed on Fas expression. CONCLUSIONS: GnRH analogues enhanced apoptosis in EEC, and this was accompanied by an increase in expression of the pro-apoptotic proteins Bax and FasL and a decrease in expression of the anti-apoptotic protein Bcl-2. SN - 0268-1161 UR - https://www.unboundmedicine.com/medline/citation/17092985/Effect_of_GnRH_analogues_on_apoptosis_and_expression_of_Bcl_2_Bax_Fas_and_FasL_proteins_in_endometrial_epithelial_cell_cultures_from_patients_with_endometriosis_and_controls_ L2 - https://academic.oup.com/humrep/article-lookup/doi/10.1093/humrep/del423 DB - PRIME DP - Unbound Medicine ER -